Dear Freesurfers,
I am using seeds based on Freesurfer masks to run a PPI analysis and am wondering if I am using reg-feat2anat at the right fsl feat registration step.

First, I ran an fsl analysis to generate a feat directory.
Then I ran reg-feat2anat using that feat directory.
Next I ran aseg2feat and fslmaths to generate binary masks
Next I extracted the timeseries by using the filtered func of the feat and the binary masks from aseg2feat.

So now I want to do another feat analysis, but this time using the extracted timeseries
But this timeseries is based on the first feat and associated masks from the reg-feat2anat registration.

1) Is it still valid to use this timeseries in this new feat since FSL will be running another registration?
2) Do I have to run reg-feat2anat again?

Thanks.
Michelle