Hello,<br><br>First of all, apologies for asking for help three times today! THings will hopefully become clearer once I get used to FS a bit; I really do appreciate the amazing support provided by the team behind FreeSurfer!<br>


<br>I ran the following command on one of my structurals:<br><br><div style="margin-left:40px"><i>recon-all -subject M01 -i M01.nii.gz -all</i><br></div><br>After a few minutes, recon-all ended with the following message:<br>


<br><div style="margin-left:40px"><i>Assertion failed at line 827 in file templates/CachedArray.cc</i><br><i>nu_estimate_np_and_em: crashed while running volume_stats (termination status=256)</i><br><i>nu_correct: crashed while running nu_estimate_np_and_em (termination status=256)</i><br>


<i>ERROR: nu_correct</i><br><i>Linux FreeSurfer 2.6.28-11-generic #42-Ubuntu SMP Fri Apr 17 01:57:59 UTC 2009 i686 GNU/Linux</i><br></div><br>I apologise for reporting an error that&#39;s already been reported in the 
past, but unfortunately the solutions suggested in those past threads 
did not work in my case. To address a few of them:<br><ul><li>my PATH (listed below) does not seem to include two MNI installations<br><i>/home/virtualuser/freesurfer/bin:/home/virtualuser/freesurfer/fsfast/bin:/home/virtualuser/freesurfer/tktools:/home/virtualuser/freesurfer/mni/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games</i></li>


<li>the version of FreeSurfer that I installed is the latest disk image available on the website (freesurfer-Virtualbox-linux-x86-stable-pub-v5.1.0-full) - I did not see any dev version that would presumably include any missing libraries presumably implied by the above error <br>


</li><li>I tried updating the Xubuntu inside my VirtualBox, however it reports an insufficient space problem that from I can see does not have a trivial fix</li></ul>Advance thanks for any help!<br>Tudor