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Hello FreeSurfer experts,

In my case-control study, I have a dataset of 5 healthy controls (HCs) and one patient (PAT). I was wondering if there is any way to perform GLM for PAT > HCs on brain morphometry data.

When I tried to follow instructions from here: MailScanner has detected a possible fraud attempt from "secure-web.cisco.com" claiming to be https://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/GroupAnalysis, the mri_glm fit command gives me the following error:

Class Size and Means of each Continuous Variable

1 HC  5  24.2000   0.6000 

2 Tumor  1  50.0000   1.0000 

ERROR: Class Tumor has 1 members. With 2 variables and using DODS, you need at least 3 members

It seems at-least three subjects are required in the Tumor category - because I have age and sex covariates in my fsgd file.

I was wondering if there is any way to still perform the above GLM analysis or any other alternatives available to compare HCs vs one PAT.

Thanks!

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