Hi Mojmir,
ASEG typically gives a larger estimate of the volume, though highly correlated with that from the subfield package. We have found the measurements from the subfield package to be a bit more reliable, though.
Cheers,
Eugenio

Juan Eugenio Iglesias
Translational Imaging Group
University College London
http://www.jeiglesias.com
http://cmictig.cs.ucl.ac.uk/


On 13 Jul 2016, at 16:07, Mojmír Vinkler <mojmir.vinkler@gmail.com> wrote:

Hi experts,

I'm using Freesurfer 6.0 dev version (Mac) to perform hippocampal subfields segmentation. I only need to measure total hippocampus volume, but measurements from basic `recon-all` were too volatile and I was hoping that using subfields with T2 modality would reduce variance. However, volume measured by hippocampal subfields is significantly different from volume in aseg.stats.

Here are volumes for right hippocampus from various methods:
aseg.stats - 3312.8
rh.hippoSfVolumes-T1.v10.txt (Mode A - only T1 used) - 2883.8
rh.hippoSfVolumes-T2.v10.txt (Mode B - using only additional scan) - 2503.97
rh.hippoSfVolumes-T1-T1T2.v10.txt (Mode B - Multispectral segmentation) - 2596.09

I checked labeling visually and there were no obvious problems. Is such a large difference common? Am I missing something? What number should be most trusted?

Thanks for any hints!
Mojmir
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