It is from an individual. I checked the registration, the green surface outline seems well within the movable EPI image. I can scale it down manually and than the zstat, with the scaled down register.dat, fits within the anatomical. Is this the only solution?

On Wed, Aug 31, 2011 at 2:34 PM, Douglas N Greve <greve@nmr.mgh.harvard.edu> wrote:
Is this a zmap from the individual run or the gfeat? If individual, double check the registration with
 tkregister2 --mov example_func.nii.gz --reg anat2exf.register.dat --surfs
If from a gfeat, then you should use anat2std.register.dat, like

tkmedit bert orig.mgz -overlay zstat1.nii.gz -overlay-reg anat2exf.register.dat -fthresh 2.3 -fmax 4.3 -aux brain.mgz -seg aparc+aseg.mgz

doug

noam Schneck wrote:
Hi,
Here is the command line for viewing the zstat from a single subject single run, in which I ran into the same problem. I've also attached a screenshot of the tkmedit image:  tkmedit bert bert/mri/orig.mgz -overlay zstat1.nii.gz -overlay-reg anat2exf.register.dat -fthresh 2.3 -fmax 4.3 -aux bert/mri/brain.mgz -seg bert/mri/aparc+aseg.mgz
Thank you so much for your help,
Noam
On Wed, Aug 31, 2011 at 12:04 PM, Douglas N Greve <greve@nmr.mgh.harvard.edu <mailto:greve@nmr.mgh.harvard.edu>> wrote:



   noam Schneck wrote:

       Hi Doug

       Here are more detailed versions of my first two questions.
       1) I used tkmedit to look at the mean_func produced by a gfeat
       analysis of one subject's three functional runs overlayed onto
       the anatomical image produced by recon-all on that same
       subject(bert/mri/orig.mgz). The registration matrix I used was
       the anat2std.dat that was produced by running reg-feat2anat on
       all three of the feat analyses from the individual runs. When
       I did this I saw that the mean_func image was outside the
       boundaries of the anatomical image. Correspondingly, when I
       overlayed the zstat.nii.gz from the gfeat analysis onto the
       anatomical image I had activation outside of the anatomical
       image brain.

   What was the command line?


       2)  Originally, I ran BBR on a subject that I had run
       Recon-all for.  I used BBR to register the example_func.nii.gz
       to the bert/mri/orig.mgz. I had BBR output an fsl.mat.  I then
       used ApplyXFM in fsl to apply BBR's fsl.mat to register the
       example_func.nii.gz onto a T1 image that had been created by
       converting bert/mri/brainmask.mgz into niftii with
       mri_convert. I tried it again, this time converting the
       orig.mgz to niftii. In both cases the example_func in T1 space
       image produced by ApplyXFM is rotated about 90 degrees and
       also inverted.  This is viewed in fslview, I have attached a
       screenshot. The corresponding T1 images created by mri_convert
       are not.  What can I do to allow me to use BBR's fsl.mat
       within ApplyXFM?

       Here is the header info for the T2*_T1 image attached to this
       email. sizeof_hdr     348
       data_type      FLOAT32
       dim0           3
       dim1           256
       dim2           256
       dim3           256
       dim4           1
       dim5           1
       dim6           1
       dim7           1
       vox_units      mm
       time_units     s
       datatype       16
       nbyper         4
       bitpix         32
       pixdim0        0.0000000000
       pixdim1        1.0000000000
       pixdim2        1.0000000000
       pixdim3        1.0000000000
       pixdim4        1.5000000000
       pixdim5        1.0000000000
       pixdim6        1.0000000000
       pixdim7        1.0000000000
       vox_offset     352
       cal_max        0.0000
       cal_min        0.0000
       scl_slope      0.000000
       scl_inter      0.000000
       phase_dim      0
       freq_dim       0
       slice_dim      0
       slice_name     Unknown
       slice_code     0
       slice_start    0
       slice_end      0
       slice_duration 0.000000
       time_offset    0.000000
       intent         Unknown
       intent_code    0
       intent_name    intent_p1      0.000000
       intent_p2      0.000000
       intent_p3      0.000000
       qform_name     Scanner Anat
       qform_code     1
       qto_xyz:1      -1.000000  -0.000000  -0.000000  128.678986
       qto_xyz:2      -0.000000  0.000000  1.000000  -100.598366
       qto_xyz:3      0.000000  -1.000000  0.000000  143.524200
       qto_xyz:4      0.000000  0.000000  0.000000  1.000000
       qform_xorient  Right-to-Left
       qform_yorient  Superior-to-Inferior
       qform_zorient  Posterior-to-Anterior
       sform_name     Scanner Anat
       sform_code     1
       sto_xyz:1      -1.000000  -0.000000  0.000000  128.678986
       sto_xyz:2      -0.000000  -0.000000  1.000000  -100.598366
       sto_xyz:3      -0.000000  -1.000000  0.000000  143.524200
       sto_xyz:4      0.000000  0.000000  0.000000  1.000000
       sform_xorient  Right-to-Left
       sform_yorient  Superior-to-Inferior
       sform_zorient  Posterior-to-Anterior
       file_type      NIFTI-1+
       file_code      1
       descrip        FreeSurfer Jun 18 2008
       aux_file      
       Thank you,
       Noam

       On Mon, Aug 29, 2011 at 1:16 PM, Douglas N Greve
       <greve@nmr.mgh.harvard.edu <mailto:greve@nmr.mgh.harvard.edu>
       <mailto:greve@nmr.mgh.harvard.edu
       <mailto:greve@nmr.mgh.harvard.edu>>> wrote:

          Hi Noam,


          noam Schneck wrote:

              Hi,

              I am trying to use reg-feat2anat to register my Feat
       analyses
              to anatomical images and running into a number of problems.
              1) When I check the registration of the mean_func to the
              anatomical in tkregister2 it looks fine. But when I overlay
              the fsl statmaps onto the anatomical brain they extend
       outside
              of boundaries of the anatomical brain.

          Need more detail. What command are you using to visualize?


              2) Also, before using reg-feat2anat. I was trying to do the
              EPI_2_structural registration in FLIRT with the fsl.mat
              produced by BBR. When I try this the registered image is
              rotated 90 degrees from the original image.  Why does
       that happen?

          More detail needed. What program did you run? What
       visualization
          tool? This will align the anatomical in the conformed space
       to the
          EPI, so make sure you're using the right anatomical.


              3) I also noticed that if I look at the header
       information for
              an image in fslhd and mri_info the orientations are
       flipped.
              If the fslhd orientation is RAS it will be LPI in mri_info.
              Could this be related to what is happening in question (2)?

          Need more detail. What images are you looking at?

          In general, you have to tell us specifically what you are
       doing.
          It's going to be very hard to help with vague descriptions.
          doug


              Best,
              Noam
                     ------------------------------------------------------------------------

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