Hi again,

the registration looks fine in tkregister2, but is apparently wrong in fslview (and also mricron). See the attached files (as link from http://vedlegg.uib.no/?id=399ad2cce1a3138f8eb4f6a06f4acfc4) showing screenshots of fslview and tkregister2. How can this be explained, and how can we visualize the correct orientation in fslview?
What does this misalignment influence any further processing with fnirt and concatenation of the affine transformation fields?

This is our command we run for bbregister:

bbregister --s subj_505 --mov vol0001.nii.gz --reg register-test.dat  --init-fsl  --t2 --o test.nii.gz

where vol0001 is the first volume in the fmri sequence and is flipped to align with LAS (as the highres).

Thank you for your kind help,

Erlend Hodneland

ean that it is in the wrong orientation? It should be in 
the same orientation as the anatomical. How does the reg look in 
tkregister2? Note that fslview can display volumes in odd orientations 
even when the orientation is correct.
doug

On 04/13/2012 03:31 AM, Erlend Hodneland wrote:
> Hi,
>
> we are trying to register fmri FEAT data (example_func.nii) to the 
> anatomical scan  (processed in Freesurfer) using bbregister to opt. 
> the affine registration before running FNIRT.
>
> However, the orientation of the registered image is flipped around and 
> is incorrect after applying bbregister.
>
> We have tried:
>
> 1)  bbregister --s subj_501 --mov example_func.nii.gz --reg 
> register.dat  --init-header --bold --o example_func2highres-fs.nii
> 2)  bbregister --s subj_501 --mov example_func.nii.gz --reg 
> register.dat  --init-fsl --bold --o example_func2highres-fs.nii
> 3)  bbregister --s subj_501  --o example_func2highres-fs.nii --feat 
> "myfeatdirectory"
> 4) tkregister2 --mov example_func.nii --regheader --reg tkreg.dat 
> (this one looks correct)
> followed by
> bbregister --s subj_501 --mov example_func.nii.gz --reg register.dat  
> --init-reg tkreg.dat --bold --o example_func2highres-fs.nii
>
> 5) reorienting to match the highres:
>
>     fslorient -swaporient example_func
>      fslswapdim example_func x -y z example_func_swap (correct 
> orientation)
>
> followed by
> bbregister --s subj_501 --mov example_func_swap.nii.gz --reg 
> register.dat  --init-fsl --bold --o example_func_swap2highres-fs.nii
>
> with the output being flipped in wrong orientation for all options.
>
> We would greatly appreciate if you have any suggestions for how to 
> overcome this problem!
>
> Cheers/Sincerly/All best
>
> Erlend Hodneland