Dear Freesurfer developers,
I have been trying to segment (with
Freesurfer v6.0.0, on Ubuntu 14.04) the hippocampal subfields of two 7T
MR images in NIFTI format: an isotropic (0.8*0.8*0.8 mm3) whole-brain
T1-weighted source volume with an anisotropic (0.3*1.2*0.3 mm3)
T2-weighted volume covering the brain partially and centred on a region
comprising both hippocampi.
I have followed the instructions in https://surfer.nmr.mgh.harvard.edu/fswiki/HighFieldRecon
to correct the bias in the two 7T volumes ('T1_biasCorrected.png',
'T2_biasCorrected.png'). I have run 'recon-all -hires [...] -expert
expert.txt' ('expert.txt') on my bias-corrected T1 volume, which
produced an accurate segmentation ('aseg.png', 'recon-all.log').
Following this, I have run 'recon-all -hippocampal-subfields-T2' on the
bias-corrected T2 volume ('hippocampal-subfields-T2.log').
Looking
at the resulting segmentation, I am unsure the molecular layer has been
properly delineated in the body of the hippocampus
('right_hippocampus_body.png', 'right_hippocampus_body_seg.png').
In addition, it appears the convolutions in the hippocampal head have
not been correctly segmented ('right_hippocampus_head.png',
'right_hippocampus_head_seg.png').
I am not certain this is
the expected behaviour when running the hippocampal subfields module on
high-res data. Is it possible the recon-all output is not accurate
enough (should I adjust the mres_inflate value prior to segmenting the
brain)? Could the high anisotropy of the T2 volume hinder the
segmentation process?
Any thoughts on this? Screenshots/attachments are available on request, as my previous message was rejected for being too big.
Thanks for your help,
Alexis