oh, sorry, the --thfile should not be the table file. The sig.mgh has the same info as the table file, so you do not need to pass the table file.

On 1/4/2022 4:44 PM, Laura Willers de Souza wrote:

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Sorry, let me explain better!

When mri_glmfit finishes it generates the sig.table.dat file which has the p values of the ROIs (first image below).

Then, when mri_fdr finishes, that same sig.table.dat file no longer contains the p-values of the ROIs, but the FDR value (second image). In this way, the p-values of the ROIs are lost.
What I would like is to end mri_fdr with a file with the value of fdr + the original sig.table.dat file with the p values of the ROIs.

Is there a way to do this?

On this link you sent there is the following:

Was it supposed to be like this?

Best,

Laura Willers de Souza

Master Student in Biochemistry

Zimmer Lab - Federal University of Rio Grande do Sul

- Porto Alegre/RS - Brazil

De: Douglas N. Greve <dgreve@mgh.harvard.edu>
Enviado: terça-feira, 4 de janeiro de 2022 17:21
Para: Laura Willers de Souza <laura.w.desouza@hotmail.com>
Cc: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Assunto: Re: [Freesurfer] Doubts about analysis of hippocampal subfields and nuclei of the amygdala
On 1/4/2022 2:42 PM, Laura Willers de Souza wrote:

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The command worked fine! But, in the end, the file that contains the thresholds for each of the ROI (first image below) ends up being replaced by the FDR value (second image below), which is not very good, because this information ends up getting lost.

I don't understand what you mean. The value in the file is the threshold that you would use to divide your ROIs into significant vs nonsig groups in a way that meets the FDR criteria; in your case, it looks all but subiculum will pass. Or are you looking for an FDR-corrected p-value for each ROI?

Is there any way to save the FDR value in another file and not in the same one where the p-values of the ROI are?

And is it possible to visualize the ROIs with the significance scale in freeview?

I tried to overlay the sig.mgh file over the inflated surface and also the mri_fdr output.mgh file, but it didn't work.

That definitely will not work.

What should I do to make this work?

You have to convert the table into a map. You can get a tool to do this from here MailScanner has detected a possible fraud attempt from "secure-web.cisco.com" claiming to be https://gate.nmr.mgh.harvard.edu/safelinks/greve/table2map
username 'guest'
password 'collab'
I'm not totally sure it will work as a stand-alone. You might have to download the development version of FS to get it. Specify the subfields segmenation as the --seg option.
Thank you so much again,

Laura Willers de Souza

Master Student in Biochemistry

Zimmer Lab - Federal University of Rio Grande do Sul

- Porto Alegre/RS - Brazil

De: Douglas N. Greve <dgreve@mgh.harvard.edu>
Enviado: terça-feira, 4 de janeiro de 2022 15:21
Para: Laura Willers de Souza <laura.w.desouza@hotmail.com>
Cc: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Assunto: Re: [Freesurfer] Doubts about analysis of hippocampal subfields and nuclei of the amygdala
that looks right; I don't think you need a 2nd --thfile (but does not hurt it).

On 1/3/2022 3:27 PM, Laura Willers de Souza wrote:
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Dear Douglas,

Once again, thanks so much for the reply!

About the mask, I just don't put anything or put "nomask"?

With your suggestions, the command looks like this:

mri_fdr --fdr .05 --pos --thfile $SUBJECTS_DIR/hipposubfields.lh.T1.v21.CU_CI.glmdir/sig.table.dat --i $SUBJECTS_DIR/hipposubfields.lh.T1.v21.CU_CI.glmdir/group.diff.CU_CI/sig.mgh nomask hipposubfields.lh.T1.v21.CU_CI.mgh
--thfile $SUBJECTS_DIR/hipposubfields.rh.T1.v21.CU_CI.glmdir/sig.table.dat --i $SUBJECTS_DIR/hipposubfields.rh.T1.v21.CU_CI.glmdir/group.diff.CU_CI/sig.mgh nomask hipposubfields.rh.T1.v21.CU_CI.mgh

Do you think this is correct?

As it was before (without the input to --thfile) it was giving the following error:

I believe that with these fixes it should work fine, right?

Thank you so much again!

Best,

Laura Willers de Souza

Master Student in Biochemistry

Zimmer Lab - Federal University of Rio Grande do Sul

- Porto Alegre/RS - Brazil
---------------------------------------------------------------------------------------------------

De: Douglas N. Greve <dgreve@mgh.harvard.edu>
Enviado: domingo, 02 de dezembro de 2022
Para: Laura Willers de Souza <laura.w.desouza@hotmail.com>
Cc: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Assunto: Re: [Freesurfer] Doubts about analysis of hippocampal subfields and nuclei of the amygdala
Dear Douglas,

Thanks so much for your answer.
Could you check if the following commands to perform ROI analysis with GLM are correct?
*1.* Create the table with the volumes of the hippocampal segmentation (1 command for each hemisphere - only replace lh with rh):
asegstats2table --statsfile hipposubfields.lh.T1.v21.stats
--fsgd CU_CI.fsgd\
--meas volume \
--all-segs \
--tablefile hipposubfields.lh.T1.v21.CU_CI.dat
*2.* GLM Analysis – DODS (1 command for each hemisphere - only replace lh with rh):
mri_glmfit \
--table hipposubfields.lh.T1.v21.CU_CI.dat \
--fsgd CU_CI.fsgddods \
--C group.diff.CU_CI.mtx \
--glmdir hipposubfields.lh.T1.v21.CU_CI.glmdir

*3.* Correction for multiple comparisons – FDR:
mri_fdr --fdr .05 --pos --thfile --i contrast/lh.sig.mgh lh.mask.mghhipposubfields.lh.T1.v21.CU_CI.mgh --no-log10p --i contrast/rh.sig.mgh rh.mask.mghhipposubfields.rh.T1.v21.CU_CI.mgh --no-log10p
For this one, you don't need a mask. Also, don't use --no-log10p (the output of mri_glmfit is in log10p). --thfile needs an argument (eg, threshold.dat)
Are these commands correct?
Another question, in this type of analysis is image smoothing common? Like was done here MailScanner has detected a possible fraud attempt from "secure-web.cisco.com" claiming to be https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg52203.html <https://secure-web.cisco.com/1we0tWxEcmAWpL06CsQ9vqTCkjuPHErSGyrotLh2MGxv3lQ9oLaGS5mPuSAbSeU01BLaT_kHew9Lf3DdE0p8vh0IaIEDCGwKQF1Ulcz8LxixmB8KkLj-1Vi6H7ciW9Rl7qpnwB3OaaAKyp1s2kuKMToWAMnsnCFVDfVBIu4wUzjLINbCXQaahGk7iCPSUCIKvCK-BA8dJGmeP8tow2PcpEF-f9eZm8laYLJJ7x8fL9lIqe_bHBt2kwhAII8fd2wBoAtwHL0Jx0YNP2NdY7WNZFwGGKG7LnbRBFoqHmeVCKsZZ1_M2y-58G2Xw3B7YsD0l/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg52203.html>
No, it would make no sense here because this is an ROI analysis
Thank you so much again!


Best regards,
*Laura Willers de Souza *
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