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Thanks! There was a mistake in the contrasts indeed.

Best

Il giorno lun 11 feb 2019 alle ore 18:59 Greve, Douglas N.,Ph.D. <DGREVE@mgh.harvard.edu> ha scritto:

If mri_diff says that there is no difference, then there is something
wrong. Double check that your contrasts are correct.


On 2/10/19 9:47 AM, Francesca Strappini wrote:
>
>         External Email - Use Caution
>
> Thanks a lot for the reply!
> If I run mri_diff order/z.nii.gz disorder/z.nii.gz I indeed get zero.
> However, if I perform the contrast "order minus disorder" I get 0.1,
> so I should see some difference although very small, right?
> These are the outputs:
>
> francesca@francesca-desktop:/opt/freesurfer/fsfast/OrderDisorder/AB/bold/AB_OrderDisorder_lh$
> mri_segstats --i order/z.nii.gz --slabel AB lh
> /opt/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label --sfavg
> lh.V2dorsal_AB_order_avg --snr --sum lh.V2dorsal_AB_order_sum
>
> $Id: mri_segstats.c,v 1.121 2016/05/31 17:27:11 greve Exp $
> cwd
> cmdline mri_segstats --i order/z.nii.gz --slabel AB lh
> /opt/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label --sfavg
> lh.V2dorsal_AB_order_avg --snr --sum lh.V2dorsal_AB_order_sum
> sysname  Linux
> hostname francesca-desktop
> machine  x86_64
> user     francesca
> UseRobust  0
> Constructing seg from label
> Loading order/z.nii.gz
> Vertex Area is 0.65781 mm^3
> Generating list of segmentation ids
> Found   2 segmentations
> Computing statistics for each segmentation
>
> Reporting on   2 segmentations
> Using PrintSegStat
> Computing spatial average of each frame
>   0  1
> Writing to lh.V2dorsal_AB_order_avg
> 0 -0.950279
> 1 1.31525
> mri_segstats done
>
> francesca@francesca-desktop:/opt/freesurfer/fsfast/OrderDisorder/AB/bold/AB_OrderDisorder_lh$
> mri_segstats --i disorder/z.nii.gz --slabel AB lh
> /opt/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label --sfavg
> lh.V2dorsal_AB_disorder_avg --snr --sum lh.V2dorsal_AB_disorder_sum
>
> $Id: mri_segstats.c,v 1.121 2016/05/31 17:27:11 greve Exp $
> cwd
> cmdline mri_segstats --i disorder/z.nii.gz --slabel AB lh
> /opt/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label --sfavg
> lh.V2dorsal_AB_disorder_avg --snr --sum lh.V2dorsal_AB_disorder_sum
> sysname  Linux
> hostname francesca-desktop
> machine  x86_64
> user     francesca
> UseRobust  0
> Constructing seg from label
> Loading disorder/z.nii.gz
> Vertex Area is 0.65781 mm^3
> Generating list of segmentation ids
> Found   2 segmentations
> Computing statistics for each segmentation
>
> Reporting on   2 segmentations
> Using PrintSegStat
> Computing spatial average of each frame
>   0  1
> Writing to lh.V2dorsal_AB_disorder_avg
> 0 -0.950279
> 1 1.31525
> mri_segstats done
>
> mri_segstats --i order-vs-disorder/z.nii.gz --slabel AB lh
> /opt/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label --sfavg
> lh.V2dorsal_AB_orderVsdisorder_avg --snr --sum
> lh.V2dorsal_AB_orderVsdisorder_sum
>
> $Id: mri_segstats.c,v 1.121 2016/05/31 17:27:11 greve Exp $
> cwd
> cmdline mri_segstats --i order-vs-disorder/z.nii.gz --slabel AB lh
> /opt/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label --sfavg
> lh.V2dorsal_AB_orderVsdisorder_avg --snr --sum
> lh.V2dorsal_AB_orderVsdisorder_sum
> sysname  Linux
> hostname francesca-desktop
> machine  x86_64
> user     francesca
> UseRobust  0
> Constructing seg from label
> Loading order-vs-disorder/z.nii.gz
> Vertex Area is 0.65781 mm^3
> Generating list of segmentation ids
> Found   2 segmentations
> Computing statistics for each segmentation
>
> Reporting on   2 segmentations
> Using PrintSegStat
> Computing spatial average of each frame
>   0  1
> Writing to lh.V2dorsal_AB_orderVsdisorder_avg
> 0 0.119339
> 1 -1.93788
> mri_segstats done
>
> Thanks again for the help!
> Best
> Francesca
>
>
>
> Il giorno mar 22 gen 2019 alle ore 20:55 Greve, Douglas N.,Ph.D.
> <DGREVE@mgh.harvard.edu <mailto:DGREVE@mgh.harvard.edu>> ha scritto:
>
>     I don't know what is happening. If the two z-maps are really
>     different,
>     then it should have given different values. Run this
>     mri_diff order/z.nii.gz disorder/z.nii.gz
>     Does it indicate that there is a difference? If so, can you run
>     your two
>     mri_segment commands and send me the terminal output of each?
>     As for the two mean intensity values, one (Seg0001) is from inside
>     your
>     ROI and the other (Seg0000) is outside. You can have it exclude
>     Seg0000
>     with --excludeid 0
>
>
>     On 1/20/19 10:00 AM, Francesca Strappini wrote:
>     >
>     >         External Email - Use Caution
>     >
>     > Dear Freesurfer's experts,
>     >
>     > I'm trying to run a ROIs mean intensity analysis using mri_segstat.
>     > I'm using as an input contrast maps created with mkcontrast-sess
>     and
>     > selxavg3-sess (block-design experiment) and as masks, some labels
>     > created on the surface with a retinotopic analysis.
>     >
>     > I noticed that all my outputs with the same label have the same
>     > values, regardless of the contrast map that I'm giving as an input.
>     > However, if I plot the contrast maps on the surface I can
>     clearly see
>     > that there are differences, so also the mean signal should be
>     different.
>     >
>     > Maybe I'm not running the command correctly:
>     >
>     > mri_segstats --i z.nii.gz --slabel sub1 lh lh.V2dorsal_sub1.label
>     > --sfavg lh.V2dorsal_sub1_condition1_avg --snr --sum
>     > lh.V2dorsal_sub1_condition1_sum
>     >
>     > These are two outputs from the same regions but with two
>     different z
>     > maps as inputs.
>     > I can also see that are indicated two different values as mean
>     > intensity, -0.95028  and 1.31525, what's the difference between
>     them?
>     >
>     > # Title Segmentation Statistics
>     > #
>     > # generating_program mri_segstats
>     > # cvs_version $Id: mri_segstats.c,v 1.75.2.9 2013/02/16 00:09:33
>     greve
>     > Exp $
>     > # cmdline mri_segstats --i disorder/z.nii.gz --slabel AB lh
>     >
>     /home/labs/malach/frastra/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label
>
>     > --sfavg lh.V2dorsal_AB_disorder_avg --snr --sum
>     > lh.V2dorsal_AB_disorder_sum
>     > # sysname  Linux
>     > # hostname access4.wexac.weizmann.ac.il
>     <http://access4.wexac.weizmann.ac.il>
>     > <http://access4.wexac.weizmann.ac.il>
>     > # machine  x86_64
>     > # user     frastra
>     > # anatomy_type surface
>     > #
>     > # SUBJECTS_DIR /home/labs/malach/frastra/freesurfer/subjects
>     > # subjectname AB
>     > # Label AB lh
>     >
>     /home/labs/malach/frastra/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label
>     > # InVolFile  disorder/z.nii.gz
>     > # InVolFileTimeStamp  2018/12/08 20:12:18
>     > # InVolFrame 0
>     > # ExcludeSegId
>     > # Only reporting non-empty segmentations
>     > # VertexArea_mm2 0.657815
>     > # TableCol  1 ColHeader Index
>     > # TableCol  1 FieldName Index
>     > # TableCol  1 Units     NA
>     > # TableCol  2 ColHeader SegId
>     > # TableCol  2 FieldName Segmentation Id
>     > # TableCol  2 Units     NA
>     > # TableCol  3 ColHeader NVertices
>     > # TableCol  3 FieldName Number of Vertices
>     > # TableCol  3 Units     unitless
>     > # TableCol  4 ColHeader Area_mm2
>     > # TableCol  4 FieldName Area
>     > # TableCol  4 Units     mm^2
>     > # TableCol  5 ColHeader Mean
>     > # TableCol  5 FieldName Intensity Mean
>     > # TableCol  5 Units     unknown
>     > # TableCol  6 ColHeader StdDev
>     > # TableCol  6 FieldName Itensity StdDev
>     > # TableCol  6 Units     unknown
>     > # TableCol  7 ColHeader Min
>     > # TableCol  7 FieldName Intensity Min
>     > # TableCol  7 Units     unknown
>     > # TableCol  8 ColHeader Max
>     > # TableCol  8 FieldName Intensity Max
>     > # TableCol  8 Units     unknown
>     > # TableCol  9 ColHeader Range
>     > # TableCol  9 FieldName Intensity Range
>     > # TableCol  9 Units     unknown
>     > # NRows 2
>     > # NTableCols 9
>     > # ColHeaders  Index SegId NVertices Area_mm2 StructName Mean StdDev
>     > Min Max Range  SNR
>     >   1   0    161272   106116.2  Seg0000 -0.9503     2.4005  -13.2314
>     > 11.4302 24.6617    -0.3959
>     >   2   1      1369      871.1  Seg0001  1.3153     3.8989   -8.2296
>     > 10.3666 18.5962     0.3373
>     >
>     >
>     >
>     > # Title Segmentation Statistics
>     > #
>     > # generating_program mri_segstats
>     > # cvs_version $Id: mri_segstats.c,v 1.75.2.9 2013/02/16 00:09:33
>     greve
>     > Exp $
>     > # cmdline mri_segstats --i order/z.nii.gz --slabel AB lh
>     >
>     /home/labs/malach/frastra/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label
>
>     > --sfavg lh.V2dorsal_AB_order_avg --snr --sum
>     lh.V2dorsal_AB_order_sum
>     > # sysname  Linux
>     > # hostname access4.wexac.weizmann.ac.il
>     <http://access4.wexac.weizmann.ac.il>
>     > <http://access4.wexac.weizmann.ac.il>
>     > # machine  x86_64
>     > # user     frastra
>     > # anatomy_type surface
>     > #
>     > # SUBJECTS_DIR /home/labs/malach/frastra/freesurfer/subjects
>     > # subjectname AB
>     > # Label AB lh
>     >
>     /home/labs/malach/frastra/freesurfer/subjects/AB/label/lh.V2dorsal_AB.label
>     > # InVolFile  order/z.nii.gz
>     > # InVolFileTimeStamp  2018/12/08 20:12:49
>     > # InVolFrame 0
>     > # ExcludeSegId
>     > # Only reporting non-empty segmentations
>     > # VertexArea_mm2 0.657815
>     > # TableCol  1 ColHeader Index
>     > # TableCol  1 FieldName Index
>     > # TableCol  1 Units     NA
>     > # TableCol  2 ColHeader SegId
>     > # TableCol  2 FieldName Segmentation Id
>     > # TableCol  2 Units     NA
>     > # TableCol  3 ColHeader NVertices
>     > # TableCol  3 FieldName Number of Vertices
>     > # TableCol  3 Units     unitless
>     > # TableCol  4 ColHeader Area_mm2
>     > # TableCol  4 FieldName Area
>     > # TableCol  4 Units     mm^2
>     > # TableCol  5 ColHeader Mean
>     > # TableCol  5 FieldName Intensity Mean
>     > # TableCol  5 Units     unknown
>     > # TableCol  6 ColHeader StdDev
>     > # TableCol  6 FieldName Itensity StdDev
>     > # TableCol  6 Units     unknown
>     > # TableCol  7 ColHeader Min
>     > # TableCol  7 FieldName Intensity Min
>     > # TableCol  7 Units     unknown
>     > # TableCol  8 ColHeader Max
>     > # TableCol  8 FieldName Intensity Max
>     > # TableCol  8 Units     unknown
>     > # TableCol  9 ColHeader Range
>     > # TableCol  9 FieldName Intensity Range
>     > # TableCol  9 Units     unknown
>     > # NRows 2
>     > # NTableCols 9
>     > # ColHeaders  Index SegId NVertices Area_mm2 StructName Mean StdDev
>     > Min Max Range  SNR
>     >   1   0    161272   106116.2  Seg0000 -0.9503     2.4005  -13.2314
>     > 11.4302 24.6617    -0.3959
>     >   2   1      1369      871.1  Seg0001  1.3153     3.8989   -8.2296
>     > 10.3666 18.5962     0.3373
>     >
>     >
>     >
>     > Thanks a lot for the help
>     > Best
>     > Francesca
>     > --
>     > Francesca Strappini, Ph.D.
>     > Neurobiology Department
>     > Weizmann Institute of Science
>     > 234 Herzl Street, Rehovot 7610001 Israel
>     > Tel.: +972 58 444 2584
>     > E-mail: francesca.strappini@weizmann.ac.il
>     <mailto:francesca.strappini@weizmann.ac.il>
>     > <mailto:francesca.strappini@weizmann.ac.il
>     <mailto:francesca.strappini@weizmann.ac.il>>
>     >
>     > _______________________________________________
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>
>
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>
>
>
> --
> Francesca Strappini, Ph.D.
> Neurobiology Department
> Weizmann Institute of Science
> 234 Herzl Street, Rehovot 7610001 Israel
> Tel.: +972 58 444 2584
> E-mail: francesca.strappini@weizmann.ac.il
> <mailto:francesca.strappini@weizmann.ac.il>
>
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--
Francesca Strappini, Ph.D.
Neurobiology Department
Weizmann Institute of Science
234 Herzl Street, Rehovot 7610001 Israel
Tel.: +972 58 444 2584
E-mail: francesca.strappini@weizmann.ac.il