If you upload the subject and send us the voxel coords we will have a look
Bruce

On Aug 12, 2014, at 10:55 PM, "Walton, Matt" <mt.walton@alumni.uleth.ca> wrote:

Hey,

Bruce, yes WM is already classified as 110 in the regions, but grey matter is still being excluded. 

Matt


On Tue, Aug 12, 2014 at 2:08 PM, Bruce Fischl <fischl@nmr.mgh.harvard.edu> wrote:
Hi Matt
Not really. Is the white matter in those regions already 110?
Bruce

On Aug 12, 2014, at 8:21 PM, "Walton, Matt" <mt.walton@alumni.uleth.ca> wrote:

Hey Bruce,

Is there anyway I can make edits to the grey matter to help with the segmentation errors (i.e reclassify grey matter in the brainmask with voxel edit so they are included in the pial layer).  Even with the addition of control points and wm edits I'm still not getting a proper segmentation, especially in areas like the temporal lobes and central grey matter where signal is lower.

Thanks,

Matt


On Thu, Aug 7, 2014 at 1:20 PM, Bruce Fischl <fischl@nmr.mgh.harvard.edu> wrote:
Hi Matt

the young age is probably why you are having to do extra work - incomplete myelination.

cheers
Bruce

On Thu, 7 Aug 2014, Walton, Matt wrote:

Thanks for your help. It is most appreciated.
I thought I would mention that these subjects are  quite young (3-4 years
old). I've read a few posts that free surfer is not ideal for analyzing
young subjects, so I am wondering if this might be causing some errors.
Maybe there is a way to register to a paediatric template, or something?

As well the raw T1w images always have an intensity bias in the occipital
lobe. I'm not sure if this is from the coil itself (32 channel phased
array), or just from the young subject age. Do you think ramping up the
normalization procedures in freesurfer would result in an improvement?

Thought I would just let you know this, incase it helps you.

Matt


On Thu, Aug 7, 2014 at 10:12 AM, Tirrell, Lee <LTIRRELL@mgh.harvard.edu>
wrote:
      Hi Matt,

      Overall, I've made the following changes that have led to some
      improvements:
      - Remove excess dura from the brainmask that hasn't been
      stripped away
      - Erasing voxels from wm in areas where white matter should be,
      even if the voxels don't show up as what on the wm mask. (If you
      view the wm volume with the heat color map, and lower the mid
      parameter to zero, areas that you have deleted show up in red) 
      - Control points to push out the pial surface

      Specifically for the case I sent you, I added control points
      near 115, 89, 82 (+/- a few slices in the sagittal view) to move
      the pial surface out further.  The wm intensity is decreasing as
      it moves out along that strand, so adding control points in
      these areas will increase the values to help Freesurfer
      recognize it as part of the white matter surface.

      Also, near 141, 116, 45 (sagittal view) there is a problem with
      the wm surface. I tried deleting wm voxels as mentioned above,
      but I don't think I was aggressive enough on the first attempt
      so it didn't help much.  I will try this again to see if it
      leads to further improvements.

      Best,
      Lee

      On 08/06/2014 11:54 AM, Walton, Matt wrote:
      Hey Bruce,
Thanks so much. If it would help, I could upload another
subject, perhaps one with worse segmentation in the occipital
lobe. I could also upload a raw T1 image sequence (Nifiti)

Matt


On Wed, Aug 6, 2014 at 9:48 AM, Tirrell, Lee
<LTIRRELL@mgh.harvard.edu> wrote:
      Hi Matt,

      I've taken a look at the subject that you uploaded. 
      I initially deleted portions of the skull from the
      brainmask that weren't removed from coronal slices
      101-109 (see screenshot), which led to some
      improvement.  I also edited the wm volume by
      deleting areas that were obviously not white matter,
      even if it didn't effect the white matter surface. 
      However, this didn't lead to much improvement.  The
      segmentation isn't as bad in this subject as in the
      screenshot that you sent originally, so this
      strategy may help in worse cases. 

      I am going to take another look at it now to try to
      improve the segmentation further.

      Best,
      Lee
           
      On 07/30/2014 12:21 PM, Walton, Matt wrote:
      Hey Bruce,
I uploaded a subject that you requested a few days
ago (problems with segmentation in the occipital
lobe). I just wanted to make sure the file
transferred successfully, and if you have had a
change to analyze.

Thanks,

Matt


On Mon, Jul 28, 2014 at 5:48 PM, Walton, Matt
<mt.walton@alumni.uleth.ca> wrote:
      Hey Bruce.
 I've sent one of my subjects in which I
noticed the segmentation problems in the
occipital lobe. Sagittal slice 115 provides a
good example. I've also noticed that with my
subjects I frequently have problems with
segmentation of the temporal lobes, although
adding control points usually fixes this quite
well.

Thanks for your help

Matt



On Mon, Jul 28, 2014 at 3:22 PM, Bruce Fischl
<fischl@nmr.mgh.harvard.edu> wrote:
      Hi Matt

      there are instructions on our
      wiki. You can either ftp or use
      the filedrop, although ftp is
      probably best if you are uploading
      an entire subject

      cheers
      Bruce
      On Mon, 28 Jul 2014, Walton, Matt
      wrote:

            Hey Bruce.
            Thanks for the
            response. How do I go
            about uploading the
            subject?

            On Monday, July 28,
            2014, Bruce Fischl
            <fischl@nmr.mgh.harvard.edu>
            wrote:
                  Hi Matt

                  certainly
            something is wrong,
            but it's hard to tell
            why from the
                  single slice.
            You have big
            topological defects
            that are being
                  fixed
            incorrectly. Can you
            upload the subject and
            we will take a
                  look?
                  cheers
                  Bruce
                  On Mon, 28 Jul
            2014, Walton, Matt
            wrote:

                        Hello
            Freesurfer team,
                        I've used
            freesurfer to segment
            (recon-all)
                       
             approximately 30
            different
                        subjects
            from a study. I've
            noticed that a large
                        number of
            these subjects
                        have a
            misclassification of
            the white and pial
                        boundaries
            in the occipital
                        lobe (such
            that grey matter is
            being classified as
                        white
            matter). I believe
                        this
            problem is originating
            from my scanner and
                        original
            data. I'm acquiring
                        a T1W
            BRAVO sequence on a GE
            Discovery 750w 3T
                        scanner,
            using a 32 channel
                        phased
            array head coil. For
            many subjects, this
                        acquistion
            gives intensity
                       
            inhomogeneity in the
            occipital lobe.  I've
            begun
                        using a GE
            based correction
                        technique
            (PURE) on the images,
            and it appears to
                        improve
            the intensity in
                        the
            occipital lobe, but
            Freesurfer is still
                        frequently
            misclassifying. 

                        I'm
            wondering what would
            be the best way to
            edit the
                        data. I've
            often tried
                        to edit
            the wm.vol, but this
            doesn't seem to fix
                        anything.
            For the most
                        part, the
            wm.vol is correct to
            begin with, but the
                        wm cutting
            line isn't
                        segmenting
            with the wm.vol. I've
            also tried to add
                        control
            points but this
                        doesn't
            seem to improve
            anything either.

                        I've
            attached an image
            which I hope will
            further
                        explain my
            problems. 

                        Thanks for
            any help



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