Dear All,

What should be my contrast vector if I want to do a direct comparison between groups (patients vs. controls) after eliminating gender and age? Should this be [1 -1]?

Should I change my FSGD in order to be able to compare between patients and controls accounting for age (I want to make sure that the age does not confound my results). Please see attached my FSGD file.

Also, my question is how the clusters are created: the clusters formation is due to a  similar value within cortical thickness(cortical thickness with similar values from patients and controls are grouped together in a cluster (e.g. cluster no.1) while other cortical thickness values are forming different clusters) or the clusters are formed based on age -age grouping (each subject belong to an age cluster)?

Thank you,

AK