I used the http://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/Visualization?highlight=%28ROI%29 to defining a region of interest, but when I make a series of left clicks on the surface£¬ then click the "Make Closed Fill Boundary" icon in the tools window. howevre, there has no connect the points my clicked, and drawing a yellow or red line between them. I hope that have experts to help me. Thanks!