Dear Experts,

We hope to acquire some multishell dwi and structural data to investigate tracts passing through the Locus Coeruleus (LC) and we want to apply the brainstem segmentation to our structural data to obtain subject specific LC masks. 

Is it possible to feed multispectral data into the brainstem segmentation ?  

Is it possible to acquire a high res volume capturing the LC in greater detail and co-register that to the whole brain T1 (T2 or FLAIR ) to further assist in the delineation of the LC roi and quantification of the LC volume? 

If it's possible to use an additional small volume like this, do I need to pre-process it using recon-all and what is the optimal way to co-register the small volume to the whole brain T1 to assist in the mask generation? 

Any guidance is very much appreciated.

Best regards and thanks,

Erik