Hi, you need to run recon-all on the T1, not on the diffusion scan. This will produce an anatomical segmentation of each subject based on the T1s, which TRACULA will then use to extract anatomical priors on white matter tracts, to aid tractography. When you run trac-all to do the actual tractography, then you run it on the diffusion scan.
From: freesurfer-bounces@nmr.mgh.harvard.edu <freesurfer-bounces@nmr.mgh.harvard.edu> on behalf of Renew Andrade <andraderenew@yahoo.com>
Sent: Friday, October 18, 2019 5:02:35 PM
To: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] Learning dti processing tutorial
 

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I am using a database of NITRC repository https://www.nitrc.org/projects/parktdi/ . This images have a nifti file for 1000s/mm^2 and 2500s/mm^2. I don’t know if it is MPRAGE or not. Can you help me?


What is the input file that you are supplying? Often this happens when 
the input it a multi-echo MPRAGE. If so, you can run
mri_concat multi-echo-mprage.mgz --rms --o mprage.mgz
then use mprage.mgz as the input to recon-all
On 10/12/2019 4:48 PM, Renew Andrade wrote:
>          External Email - Use Caution
>
> Dear freesurfer experts:
> I am trying to learn dti processing. For using Tracula it appears there is a 
> need for running "recon-all -all -i -s” on every subject before starting to 
> run Tracula. But there seems to be a problem. I have a Parkinson dwi images 
> database and every subject gives me an error of the type “input(s) cannot 
> have multiple frames!”. I am a little bit stuck on this step. Is it a problem 
> of the file as input? Am I putting the wrong file? Can it be done a recon-all 
> analysis to a dwi image? With trackvis I can obtain the results without any 
> problem. My doubt comes from FreeSurfer only and may be my little knowledge 
> about it.
> Thanks for your help!
> Sincerely,
> Andrade.
>
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