On 2/18/2021 7:06 AM, Jayachandra Raghava wrote:

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Hello Freesurfer Experts,

I am using Freesurfer version 7.1.1 to perform longitudinal Samseg processing (using run_samseg_long) on my T1 longitudinal data.

1) First, I run the mri_robust_template to generate the mean.mgz using T1.mgz from the cross sectional recon-all pipeline. Would it be better to use T1.mgz from the longitudinally processed data?
You mean the T1.mgz from the recon-all output? Don't use that. Use the orig.mgz.from the long stream (you'd only need to run it long enough to produce the orig.mgz)

2) There are few subjects who do not have followup data. How do I process these subjects to avoid the bias introduced by the longitudinal Samseg pipeline?
You can run the recon-all long stream on that one time point;  it will apply a registration designed to not introduce bias, then use the orig.mgz

3) For the same subject, I see that the subcortical volumes from longitudinal recon-all pipeline from aseg.stats (e.g Left-Hippocampus volume is 3321.5mm3) are different to the subcortical volumes from longitudinal samseg pipeline from samseg.stats (e.g Left-Hippocampus volume is 3899.628686mm3). Which of these measures would be recommended?
In general I would say samseg, but that is a pretty big difference. Can you take a look and see if one looks better than the other?

Thank you very much for your support!

Best wishes
Jay

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