Thank you Douglas for your reply.

I have perforemed

surfreg --s subj1 --t fsaverage_sym --lh 
surfreg --s subj1--t fsaverage_sym --lh --xhemi
mris_preproc --target fsaverage_sym --hemi lh --xhemi --paired-diff  --srcsurfreg fsaverage_sym.sphere.reg  --meas thickness  --out lh.lh-rh.thickness.sm00.mgh  --s subj1

for my one subject. as I understand "lh.lh-rh.thickness.sm00.mgh" file is the difference between the two hemisphere of my one subject (it's dimension is 163842 x 1 x 1).  so I should check if these values are significantly different than zero, right?

but DOF of this vector is 0 so  mri_glmfit returns "ERROR: DOF = 0". which is correct I think. 

I believe I can't do this procedure for single subject. I want to check if there is thickening or thinning between two hemisphere for one subject. what should I do exactly?

Regards,
Ashkan

--
-- 
Ashkan Faghiri
MSc student of Bioelectrical Engineering
Department of Electrical Engineering
Sharif University of Technology