Hi - 

I would really like to use a volume size greater than 256^3.

I know of all the options and reasons.

what I want is a white matter surface than has fairly evenly spaced nodes that are in approximate match to the voxels they enclose.

the reason is to seed, track and target using FSL surface tractography. the brain is a 250um macaque, and it's 320 voxels long unfortunately. the structural and DWI are the same resolution and in register and acpc aligned. such a shame to waste.

the freesurfer surfaces are imported into CARET and cross-registered to F99. but they keep their mesh if I want.

work with another sample showed that node numbers and even coverage over the WM/GM boundary had a signifiant effect on the FSL metrics, with the original FS WM surface and derivatives yielding the best results over other meshes like F99's or anything made with surefit directly from a downsampled version of the data. that data I could fit into 256^3.

I doubt that the nodes and voxels need to match perfectly, just ballpark and fairly even spacing.

if I used a downsampled volume at 500um to make surfaces in freesurfer, maybe I could upsmaple the surfaces afterwards? but then the pial and white would have to be resampled independently and that's probably no good.

If I have to resample the volume or apply a scaling transform the world won't end. In fact it will take less time to do probably. But it would be nice to use a larger size than 256^3 to take advantage of what I've got.

best,

Colin, Sussex

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