Thank you for your kind reply. Sorry for the confusing question. Now I have an annotation file in fsaverage space. And I want to extracted mean time series from each roi in this annotation file from preprocessed fMRI data, e.g. fmc.odd.sm5.fsaverage.lh.nii.gz. I think the fcseed-config and fcseed-sess works when I input the volume data such as fmc.odd.nii.gz, namely in the individual space. I have tried transform this annotation file to the individual segmentation file, however, the segmentation was wrong for each roi. May I ask if it is available to extract time series from the surface data with the custom annotation file in the fsaverage space? I am not clear if I could use mri_segstats to achieve this, I found a example of this command in individual space "mri_segstats --seg aseg-in-func.img --ctab $FREESURFER_HOME/FreeSurferColorLUT.txt --excludeid 0 --i func.img --mask spmT.img --maskthresh 2.3 --sum bert.aseg-in-func.sum --avgwf bert.avgwf.dat --avgwfvol bert.avgwf.img".  How could I apply it into the surface data? Any suggestions would be appreciated.

Best wishes,

Zhi