Hello,Question 1: I would like to conduct an Unpaired T-test group analysis, I have two group of people, Group A8 age is 8 and Group A9 age is 9, and I to test the change in thickness with age. But I am little confusion about the matirx and FSGD design. I am appreciated if you can take a look at my FSGD file and Matrix file. Please correct me if I am wrong,Question2 : I am using tksurfer to analysis the brain thickness, and I have a question about setting threshold, for I am not so sure about the relation between threshold and Min.is the equation Min = -log10(threshold) correct?(https://surfer.nmr.mgh.harvard.edu/fswiki/ )FsTutorial/Visualization For example, if I want the threshold to be 0.05, and the Min will be set to 1.3Please see the image attached in this email, and correct me if I am wrong.Thank you!Jincheng