Dear all,<div><br></div><div>I search my question before I post it. I did find similar questions, but didn&#39;t find answer. I want to overlay two files with different colors. For example, one is sig1.mgh from cortical thickness, the other is sig2.mgh from area. I want to use tksurfer overlaying them with three colors: red is significant regions of thickness; blue is significant regions of area; green is overlap significant regions of thickness and area.  </div>
<div><br></div><div>I tried to use the following command:</div><div><br></div><div>tksurfer fsaverage lh inflated -annot aparc.annot -fthresh 2 -overlay sig1.mgh  -overlay sig2.mgh</div><div><br></div><div>It just can display sig1.mgh or sig2.mgh, but can&#39;t display two together. In addition, I don&#39;t know how to control overlap and other regions color.</div>
<div><br></div><div>Anyone knows how to do this?</div><div><br></div><div>Any help will be appreciated!</div><div><br></div><div>Thanks in advance!</div><div><br></div><div>Kathy </div>