Freesurfer July 2013

freesurfer@nmr.mgh.harvard.edu

165 participants
332 discussions

Tracts appear to be too large and malformed.
by Susan Kuo 03 Jul '13

03 Jul '13

Hi Anastasia, I am reviewing some of the subject's tracts - specifically looking at the uncinate fasciculus, and I noticed that some of the tracts look malformed. They look "chubby" and overgrown in some cases. Can you suggest a reason why this might be, and what I can do to control for this? Attached is a jpg showing the uncinate fasciculus in either hemisphere. As you can see, the right side is considerably larger and poorly defined. -- Susie Kuo Mediocrity knows nothing higher than itself, but talent instantly recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of Fear*

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Uncinate tract appears to be malformed
by Susan Kuo 03 Jul '13

03 Jul '13

Hi Anastasia, I am reviewing some of the subject's tracts - specifically looking at the uncinate fasciculus, and I noticed that some of the tracts look malformed. They look "chubby" and overgrown in some cases. Can you suggest a reason why this might be, and what I can do to control for this? Attached is a jpg showing the uncinate fasciculus in either hemisphere. As you can see, the right side is considerably larger and poorly defined. I'm also attaching a tgz file that contain all the files in <subject_dir>/dpath/rh.unc_AS_avg33_mni_bbr, for your perusal. Any help you can offer will be greatly appreciated by me! Thank you! -- Susie Kuo Mediocrity knows nothing higher than itself, but talent instantly recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of Fear*

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Re: [Freesurfer] Tracula/ config file error
by Anastasia Yendiki 03 Jul '13

03 Jul '13

What is the trac-all command line that you're running? On Wed, 3 Jul 2013, Gundran, Andrew wrote: > I dont seem to have a trac-all.log file in the scripts directory. I do however have a recon-all.log. I'll attach > ________________________________________ > From: freesurfer-bounces(a)nmr.mgh.harvard.edu [freesurfer-bounces(a)nmr.mgh.harvard.edu] On Behalf Of Anastasia Yendiki [ayendiki(a)nmr.mgh.harvard.edu] > Sent: Wednesday, July 03, 2013 2:42 PM > To: Gundran, Andrew > Cc: freesurfer(a)nmr.mgh.harvard.edu > Subject: Re: [Freesurfer] Tracula/ config file error > > Can you please send the trac-all.log file from the scripts directory? > > On Wed, 3 Jul 2013, Gundran, Andrew wrote: > >> That'd be a yes to both your questions. >> >> -----Original Message----- >> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >> Sent: Wednesday, July 03, 2013 2:33 PM >> To: Gundran, Andrew >> Cc: freesurfer(a)nmr.mgh.harvard.edu >> Subject: RE: [Freesurfer] Tracula/ config file error >> >> >> You're very welcome. Is your freesurfer recon-all output for this subject under /home/baileype/Desktop/JG005/freesurfer? Does the file mri/aparc+aseg.mgz exist? >> >> I'm cc-ing the freesurfer list so that our conversation is archived, other users benefit from it, and we avoid replication of questions. >> >> On Wed, 3 Jul 2013, Gundran, Andrew wrote: >> >>> I've reattched the actual file so it might be easier. >>> Thanks for all the help. >>> ________________________________________ >>> From: Anastasia Yendiki [ayendiki(a)nmr.mgh.harvard.edu] >>> Sent: Monday, July 01, 2013 3:38 PM >>> To: Gundran, Andrew >>> Cc: freesurfer(a)nmr.mgh.harvard.edu >>> Subject: RE: [Freesurfer] Tracula/ config file error >>> >>> That probably means you're not running a C shell. That's fine, can you >>> instead try running trac-all and see if it prints out the value of >>> dcmlist now that you've added that line? >>> >>> I'll repeat my other question: What program are you using to view the >>> text file? >>> >>> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >>> >>>> Whoops. It doesn't read like that every # is a new line along with each "set" >>>> >>>> I did that and the error was >>>> >>>> Line13: syntax error near unexpected token `(' >>>> Line 13: `set subjlist = (freesurfer) >>>> >>>> >>>> -----Original Message----- >>>> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >>>> Sent: Monday, July 01, 2013 3:09 PM >>>> To: Gundran, Andrew >>>> Cc: freesurfer(a)nmr.mgh.harvard.edu >>>> Subject: RE: [Freesurfer] Tracula/ config file error >>>> >>>> >>>> What program are you viewing it in? If you add a line at the end of config.txt that says "echo $dcmlist" (without the quotation marks) and then run "source config.txt" on the command line, does it print out "DKI.nii.gz"? >>>> >>>> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >>>> >>>>> It reads like >>>>> >>>>> # Input diffusion DICOMs (file names relative to dcmroot) # If >>>>> original DICOMs don't exist, these can be in other image format # >>>>> but then bvecfile and bvalfile must be specified (see below) # set >>>>> dcmroot = /home/baileype/Desktop/JG005 set dcmlist = (DKI.nii.gz) >>>>> >>>>> -----Original Message----- >>>>> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >>>>> Sent: Monday, July 01, 2013 2:48 PM >>>>> To: Gundran, Andrew >>>>> Cc: freesurfer(a)nmr.mgh.harvard.edu >>>>> Subject: RE: [Freesurfer] Tracula/ config file error >>>>> >>>>> >>>>> Can you see that this is all one line? >>>>> >>>>> # Input diffusion DICOMs (file names relative to dcmroot) # If >>>>> original DICOMs don't exist, these can be in other image format # >>>>> but then bvecfile and bvalfile must be specified (see below) # set >>>>> dcmroot = >>>>> /home/baileype/Desktop/JG005 set dcmlist = (DKI.nii.gz) >>>>> >>>>> >>>>> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >>>>> >>>>>> Yes, the text was saved on a unix system >>>>>> >>>>>> -----Original Message----- >>>>>> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >>>>>> Sent: Monday, July 01, 2013 2:34 PM >>>>>> To: Gundran, Andrew >>>>>> Cc: freesurfer(a)nmr.mgh.harvard.edu >>>>>> Subject: Re: [Freesurfer] Tracula/ config file error >>>>>> >>>>>> >>>>>> Hi Andrew - I'm sorry that you're annoyed. I can feel your pain. Take a deep breath and let it all go. >>>>>> >>>>>> More to the point, when I view the text file that you sent, I see that several of the lines are joined together, so both the dcmroot and dcmlist variables appear commented out. Is this intentional? Was this text file saved on a unix system? >>>>>> >>>>>> a.y >>>>>> >>>>>> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >>>>>> >>>>>>> >>>>>>> I have been trying to run tracula with a config file but keep >>>>>>> getting the same error message when I use the trac-all command for >>>>>>> pre-processing >>>>>>> >>>>>>> "set: Variable name must begin with a letter" >>>>>>> >>>>>>> >>>>>>> >>>>>>> It has become very, very annoying. PLEASE SOMEONE HELP!!! >>>>>>> >>>>>>> >>>>>>> >>>>>>> Andrew >>>>>>> >>>>>>> >>>>>>> >>>>>>> ------ >>>>>>> >>>>>>> # FreeSurfer SUBJECTS_DIR >>>>>>> >>>>>>> # /home/baileype/Desktop/JG005/freesurfer/mri >>>>>>> >>>>>>> # >>>>>>> >>>>>>> setenv SUBJECTS_DIR /home/baileype/Desktop/JG005 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Output directory where trac-all results will be saved # Default: >>>>>>> Same as SUBJECTS_DIR # set dtroot = /home/baileype/Desktop/JG005 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Subject IDs >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set subjlist = (freesurfer) >>>>>>> >>>>>>> >>>>>>> >>>>>>> # In case you want to analyze only Huey and Louie # Default: Run >>>>>>> analysis on all subjects # set runlist = (1) >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Input diffusion DICOMs (file names relative to dcmroot) # If >>>>>>> original DICOMs don't exist, these can be in other image format # >>>>>>> but then bvecfile and bvalfile must be specified (see below) # set >>>>>>> dcmroot = >>>>>>> /home/baileype/Desktop/JG005 set dcmlist = (DKI.nii.gz) >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Diffusion gradient table >>>>>>> >>>>>>> # Must be specified if inputs are not MGH DICOMs # Three-column >>>>>>> format, one row for each volume in the diffusion data set # Default: >>>>>>> Read from DICOM header # set bvecfile = >>>>>>> /home/baileype/Desktop/JG005/bvecs >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Diffusion b-value table >>>>>>> >>>>>>> # Must be specified if inputs are not MGH DICOMs # Single-column >>>>>>> format, one value for each volume in the diffusion data set # Default: >>>>>>> Read from DICOM header # set bvalfile = >>>>>>> /home/baileype/Desktop/JG005/bvals >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Perform registration-based B0-inhomogeneity compensation? >>>>>>> >>>>>>> # Default: 0 (no) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> # set dob0 = 1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Input B0 field map magnitude DICOMs (file names relative to >>>>>>> dcmroot) # Only used if dob0 = 1 # Default: None # # set b0mlist = >>>>>>> (huey/fmag/XXX-1.dcm dewey/fmag/XXX-1.dcm louie/fmag/XXX-1.dcm) >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Input B0 field map phase DICOMs (file names relative to dcmroot) >>>>>>> # Only used if dob0 = 1 # Default: None # # set b0plist = >>>>>>> (huey/fphas/XXX-1.dcm dewey/fphas/XXX-1.dcm louie/fphas/XXX-1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Echo spacing for field mapping sequence (from sequence printout) >>>>>>> # Only used if dob0 = 1 # Default: None # # set echospacing = 0.7 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Perform registration-based eddy-current compensation? >>>>>>> >>>>>>> # Default: 1 (yes) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set doeddy = 1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Rotate diffusion gradient vectors to match eddy-current compensation? >>>>>>> >>>>>>> # Only used if doeddy = 1 >>>>>>> >>>>>>> # Default: 1 (yes) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set dorotbvecs = 1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Fractional intensity threshold for BET mask extraction from >>>>>>> low-b images # This mask is used only if usemaskanat = 0 # >>>>>>> Default: 0.3 # set thrbet = 0.2 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Perform diffusion-to-T1 registration by flirt? >>>>>>> >>>>>>> # Default: 0 (no) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set doregflt = 0 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Perform diffusion-to-T1 registration by bbregister? >>>>>>> >>>>>>> # Default: 1 (yes) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set doregbbr = 1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Perform registration of T1 to MNI template? >>>>>>> >>>>>>> # Default: 1 (yes) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set doregmni = 1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # MNI template >>>>>>> >>>>>>> # Only used if doregmni = 1 >>>>>>> >>>>>>> # Default: $FSLDIR/data/standard/MNI152_T1_1mm_brain.nii.gz >>>>>>> >>>>>>> # >>>>>>> >>>>>>> # set mnitemp = /path/to/mni_template.nii.gz >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Perform registration of T1 to CVS template? >>>>>>> >>>>>>> # Default: 0 (no) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> # set doregcvs = 0 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # CVS template subject ID >>>>>>> >>>>>>> # Only used if doregcvs = 1 >>>>>>> >>>>>>> # Default: cvs_avg35 >>>>>>> >>>>>>> # >>>>>>> >>>>>>> # set cvstemp = donald >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Parent directory of the CVS template subject # Only used if >>>>>>> doregcvs = 1 # >>>>>>> Default: $FREESURFER_HOME/subjects # # set cvstempdir = >>>>>>> /path/to/cvs/atlases/of/ducks >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Use brain mask extracted from T1 image instead of low-b diffusion image? >>>>>>> >>>>>>> # Has no effect if there is no T1 data >>>>>>> >>>>>>> # Default: 1 (yes) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set usemaskanat = 1 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Paths to reconstruct >>>>>>> >>>>>>> # Default: All paths in the atlas >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set pathlist = (lh.cst_AS rh.cst_AS \ >>>>>>> >>>>>>> lh.unc_AS rh.unc_AS \ >>>>>>> >>>>>>> lh.ilf_AS rh.ilf_AS \ >>>>>>> >>>>>>> fmajor_PP fminor_PP \ >>>>>>> >>>>>>> lh.atr_PP rh.atr_PP \ >>>>>>> >>>>>>> lh.ccg_PP rh.ccg_PP \ >>>>>>> >>>>>>> lh.cab_PP rh.cab_PP \ >>>>>>> >>>>>>> lh.slfp_PP rh.slfp_PP \ >>>>>>> >>>>>>> lh.slft_PP rh.slft_PP) >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Number of path control points >>>>>>> >>>>>>> # It can be a single number for all paths or a different number >>>>>>> for each of the # paths specified in pathlist # Default: 7 for the >>>>>>> forceps major, 6 for the corticospinal tract, >>>>>>> >>>>>>> # 4 for the angular bundle, and 5 for all other paths >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set ncpts = (6 6 5 5 5 5 7 5 5 5 5 5 4 4 5 5 5 5) >>>>>>> >>>>>>> >>>>>>> >>>>>>> # List of training subjects >>>>>>> >>>>>>> # This text file lists the locations of training subject >>>>>>> directories # >>>>>>> Default: $FREESURFER_HOME/trctrain/trainlist.txt >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set trainfile = $FREESURFER_HOME/trctrain/trainlist.txt >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Number of "sticks" (anisotropic diffusion compartments) in the >>>>>>> bedpostx # ball-and-stick model # Default: 2 # set nstick = 2 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Number of MCMC burn-in iterations >>>>>>> >>>>>>> # (Path samples drawn initially by MCMC algorithm and discarded) # Default: >>>>>>> 200 # set nburnin = 200 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Number of MCMC iterations >>>>>>> >>>>>>> # (Path samples drawn by MCMC algorithm and used to estimate path >>>>>>> distribution) # Default: 7500 # set nsample = 7500 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Frequency with which MCMC path samples are retained for path >>>>>>> distribution # Default: 5 (keep every 5th sample) # set nkeep = 5 >>>>>>> >>>>>>> >>>>>>> >>>>>>> # Reinitialize path reconstruction? >>>>>>> >>>>>>> # This is an option of last resort, to be used only if one of the >>>>>>> reconstructed # pathway distributions looks like a single curve. >>>>>>> This is a sign that the # initial guess for the pathway was >>>>>>> problematic, perhaps due to poor alignment # between the individual and the atlas. >>>>>>> Setting the reinit parameter to 1 and # rerunning "trac-all -prior" >>>>>>> and "trac-all -path", only for the specific # subjects and >>>>>>> pathways that had this problem, will attempt to reconstruct them # with a different initial guess. >>>>>>> >>>>>>> # Default: 0 (do not reinitialize) >>>>>>> >>>>>>> # >>>>>>> >>>>>>> set reinit = 0 >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>>> >>>>>> >>>>>> >>>>>> The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. >>>>>> >>>>>> >>>>>> >>>>> >>>>> >>>>> >>>> >>>> >>>> >>> >> >> >> > _______________________________________________ > Freesurfer mailing list > Freesurfer(a)nmr.mgh.harvard.edu > https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer >

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Re: [Freesurfer] Tracula/ config file error
by Anastasia Yendiki 03 Jul '13

03 Jul '13

You're very welcome. Is your freesurfer recon-all output for this subject under /home/baileype/Desktop/JG005/freesurfer? Does the file mri/aparc+aseg.mgz exist? I'm cc-ing the freesurfer list so that our conversation is archived, other users benefit from it, and we avoid replication of questions. On Wed, 3 Jul 2013, Gundran, Andrew wrote: > I've reattched the actual file so it might be easier. > Thanks for all the help. > ________________________________________ > From: Anastasia Yendiki [ayendiki(a)nmr.mgh.harvard.edu] > Sent: Monday, July 01, 2013 3:38 PM > To: Gundran, Andrew > Cc: freesurfer(a)nmr.mgh.harvard.edu > Subject: RE: [Freesurfer] Tracula/ config file error > > That probably means you're not running a C shell. That's fine, can you > instead try running trac-all and see if it prints out the value of > dcmlist now that you've added that line? > > I'll repeat my other question: What program are you using to view the text > file? > > On Mon, 1 Jul 2013, Gundran, Andrew wrote: > >> Whoops. It doesn't read like that every # is a new line along with each "set" >> >> I did that and the error was >> >> Line13: syntax error near unexpected token `(' >> Line 13: `set subjlist = (freesurfer) >> >> >> -----Original Message----- >> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >> Sent: Monday, July 01, 2013 3:09 PM >> To: Gundran, Andrew >> Cc: freesurfer(a)nmr.mgh.harvard.edu >> Subject: RE: [Freesurfer] Tracula/ config file error >> >> >> What program are you viewing it in? If you add a line at the end of config.txt that says "echo $dcmlist" (without the quotation marks) and then run "source config.txt" on the command line, does it print out "DKI.nii.gz"? >> >> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >> >>> It reads like >>> >>> # Input diffusion DICOMs (file names relative to dcmroot) # If >>> original DICOMs don't exist, these can be in other image format # but >>> then bvecfile and bvalfile must be specified (see below) # set dcmroot >>> = /home/baileype/Desktop/JG005 set dcmlist = (DKI.nii.gz) >>> >>> -----Original Message----- >>> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >>> Sent: Monday, July 01, 2013 2:48 PM >>> To: Gundran, Andrew >>> Cc: freesurfer(a)nmr.mgh.harvard.edu >>> Subject: RE: [Freesurfer] Tracula/ config file error >>> >>> >>> Can you see that this is all one line? >>> >>> # Input diffusion DICOMs (file names relative to dcmroot) # If >>> original DICOMs don't exist, these can be in other image format # but >>> then bvecfile and bvalfile must be specified (see below) # set dcmroot >>> = >>> /home/baileype/Desktop/JG005 set dcmlist = (DKI.nii.gz) >>> >>> >>> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >>> >>>> Yes, the text was saved on a unix system >>>> >>>> -----Original Message----- >>>> From: Anastasia Yendiki [mailto:ayendiki@nmr.mgh.harvard.edu] >>>> Sent: Monday, July 01, 2013 2:34 PM >>>> To: Gundran, Andrew >>>> Cc: freesurfer(a)nmr.mgh.harvard.edu >>>> Subject: Re: [Freesurfer] Tracula/ config file error >>>> >>>> >>>> Hi Andrew - I'm sorry that you're annoyed. I can feel your pain. Take a deep breath and let it all go. >>>> >>>> More to the point, when I view the text file that you sent, I see that several of the lines are joined together, so both the dcmroot and dcmlist variables appear commented out. Is this intentional? Was this text file saved on a unix system? >>>> >>>> a.y >>>> >>>> On Mon, 1 Jul 2013, Gundran, Andrew wrote: >>>> >>>>> >>>>> I have been trying to run tracula with a config file but keep >>>>> getting the same error message when I use the trac-all command for >>>>> pre-processing >>>>> >>>>> "set: Variable name must begin with a letter" >>>>> >>>>> >>>>> >>>>> It has become very, very annoying. PLEASE SOMEONE HELP!!! >>>>> >>>>> >>>>> >>>>> Andrew >>>>> >>>>> >>>>> >>>>> ------ >>>>> >>>>> # FreeSurfer SUBJECTS_DIR >>>>> >>>>> # /home/baileype/Desktop/JG005/freesurfer/mri >>>>> >>>>> # >>>>> >>>>> setenv SUBJECTS_DIR /home/baileype/Desktop/JG005 >>>>> >>>>> >>>>> >>>>> # Output directory where trac-all results will be saved # Default: >>>>> Same as SUBJECTS_DIR # set dtroot = /home/baileype/Desktop/JG005 >>>>> >>>>> >>>>> >>>>> # Subject IDs >>>>> >>>>> # >>>>> >>>>> set subjlist = (freesurfer) >>>>> >>>>> >>>>> >>>>> # In case you want to analyze only Huey and Louie # Default: Run >>>>> analysis on all subjects # set runlist = (1) >>>>> >>>>> >>>>> >>>>> # Input diffusion DICOMs (file names relative to dcmroot) # If >>>>> original DICOMs don't exist, these can be in other image format # >>>>> but then bvecfile and bvalfile must be specified (see below) # set >>>>> dcmroot = >>>>> /home/baileype/Desktop/JG005 set dcmlist = (DKI.nii.gz) >>>>> >>>>> >>>>> >>>>> # Diffusion gradient table >>>>> >>>>> # Must be specified if inputs are not MGH DICOMs # Three-column >>>>> format, one row for each volume in the diffusion data set # Default: >>>>> Read from DICOM header # set bvecfile = >>>>> /home/baileype/Desktop/JG005/bvecs >>>>> >>>>> >>>>> >>>>> # Diffusion b-value table >>>>> >>>>> # Must be specified if inputs are not MGH DICOMs # Single-column >>>>> format, one value for each volume in the diffusion data set # Default: >>>>> Read from DICOM header # set bvalfile = >>>>> /home/baileype/Desktop/JG005/bvals >>>>> >>>>> >>>>> >>>>> # Perform registration-based B0-inhomogeneity compensation? >>>>> >>>>> # Default: 0 (no) >>>>> >>>>> # >>>>> >>>>> # set dob0 = 1 >>>>> >>>>> >>>>> >>>>> # Input B0 field map magnitude DICOMs (file names relative to >>>>> dcmroot) # Only used if dob0 = 1 # Default: None # # set b0mlist = >>>>> (huey/fmag/XXX-1.dcm dewey/fmag/XXX-1.dcm louie/fmag/XXX-1.dcm) >>>>> >>>>> >>>>> >>>>> # Input B0 field map phase DICOMs (file names relative to dcmroot) # >>>>> Only used if dob0 = 1 # Default: None # # set b0plist = >>>>> (huey/fphas/XXX-1.dcm dewey/fphas/XXX-1.dcm louie/fphas/XXX-1 >>>>> >>>>> >>>>> >>>>> # Echo spacing for field mapping sequence (from sequence printout) # >>>>> Only used if dob0 = 1 # Default: None # # set echospacing = 0.7 >>>>> >>>>> >>>>> >>>>> # Perform registration-based eddy-current compensation? >>>>> >>>>> # Default: 1 (yes) >>>>> >>>>> # >>>>> >>>>> set doeddy = 1 >>>>> >>>>> >>>>> >>>>> # Rotate diffusion gradient vectors to match eddy-current compensation? >>>>> >>>>> # Only used if doeddy = 1 >>>>> >>>>> # Default: 1 (yes) >>>>> >>>>> # >>>>> >>>>> set dorotbvecs = 1 >>>>> >>>>> >>>>> >>>>> # Fractional intensity threshold for BET mask extraction from low-b >>>>> images # This mask is used only if usemaskanat = 0 # Default: 0.3 # >>>>> set thrbet = 0.2 >>>>> >>>>> >>>>> >>>>> # Perform diffusion-to-T1 registration by flirt? >>>>> >>>>> # Default: 0 (no) >>>>> >>>>> # >>>>> >>>>> set doregflt = 0 >>>>> >>>>> >>>>> >>>>> # Perform diffusion-to-T1 registration by bbregister? >>>>> >>>>> # Default: 1 (yes) >>>>> >>>>> # >>>>> >>>>> set doregbbr = 1 >>>>> >>>>> >>>>> >>>>> # Perform registration of T1 to MNI template? >>>>> >>>>> # Default: 1 (yes) >>>>> >>>>> # >>>>> >>>>> set doregmni = 1 >>>>> >>>>> >>>>> >>>>> # MNI template >>>>> >>>>> # Only used if doregmni = 1 >>>>> >>>>> # Default: $FSLDIR/data/standard/MNI152_T1_1mm_brain.nii.gz >>>>> >>>>> # >>>>> >>>>> # set mnitemp = /path/to/mni_template.nii.gz >>>>> >>>>> >>>>> >>>>> # Perform registration of T1 to CVS template? >>>>> >>>>> # Default: 0 (no) >>>>> >>>>> # >>>>> >>>>> # set doregcvs = 0 >>>>> >>>>> >>>>> >>>>> # CVS template subject ID >>>>> >>>>> # Only used if doregcvs = 1 >>>>> >>>>> # Default: cvs_avg35 >>>>> >>>>> # >>>>> >>>>> # set cvstemp = donald >>>>> >>>>> >>>>> >>>>> # Parent directory of the CVS template subject # Only used if >>>>> doregcvs = 1 # >>>>> Default: $FREESURFER_HOME/subjects # # set cvstempdir = >>>>> /path/to/cvs/atlases/of/ducks >>>>> >>>>> >>>>> >>>>> # Use brain mask extracted from T1 image instead of low-b diffusion image? >>>>> >>>>> # Has no effect if there is no T1 data >>>>> >>>>> # Default: 1 (yes) >>>>> >>>>> # >>>>> >>>>> set usemaskanat = 1 >>>>> >>>>> >>>>> >>>>> # Paths to reconstruct >>>>> >>>>> # Default: All paths in the atlas >>>>> >>>>> # >>>>> >>>>> set pathlist = (lh.cst_AS rh.cst_AS \ >>>>> >>>>> lh.unc_AS rh.unc_AS \ >>>>> >>>>> lh.ilf_AS rh.ilf_AS \ >>>>> >>>>> fmajor_PP fminor_PP \ >>>>> >>>>> lh.atr_PP rh.atr_PP \ >>>>> >>>>> lh.ccg_PP rh.ccg_PP \ >>>>> >>>>> lh.cab_PP rh.cab_PP \ >>>>> >>>>> lh.slfp_PP rh.slfp_PP \ >>>>> >>>>> lh.slft_PP rh.slft_PP) >>>>> >>>>> >>>>> >>>>> # Number of path control points >>>>> >>>>> # It can be a single number for all paths or a different number for >>>>> each of the # paths specified in pathlist # Default: 7 for the >>>>> forceps major, 6 for the corticospinal tract, >>>>> >>>>> # 4 for the angular bundle, and 5 for all other paths >>>>> >>>>> # >>>>> >>>>> set ncpts = (6 6 5 5 5 5 7 5 5 5 5 5 4 4 5 5 5 5) >>>>> >>>>> >>>>> >>>>> # List of training subjects >>>>> >>>>> # This text file lists the locations of training subject directories >>>>> # >>>>> Default: $FREESURFER_HOME/trctrain/trainlist.txt >>>>> >>>>> # >>>>> >>>>> set trainfile = $FREESURFER_HOME/trctrain/trainlist.txt >>>>> >>>>> >>>>> >>>>> # Number of "sticks" (anisotropic diffusion compartments) in the >>>>> bedpostx # ball-and-stick model # Default: 2 # set nstick = 2 >>>>> >>>>> >>>>> >>>>> # Number of MCMC burn-in iterations >>>>> >>>>> # (Path samples drawn initially by MCMC algorithm and discarded) # Default: >>>>> 200 # set nburnin = 200 >>>>> >>>>> >>>>> >>>>> # Number of MCMC iterations >>>>> >>>>> # (Path samples drawn by MCMC algorithm and used to estimate path >>>>> distribution) # Default: 7500 # set nsample = 7500 >>>>> >>>>> >>>>> >>>>> # Frequency with which MCMC path samples are retained for path >>>>> distribution # Default: 5 (keep every 5th sample) # set nkeep = 5 >>>>> >>>>> >>>>> >>>>> # Reinitialize path reconstruction? >>>>> >>>>> # This is an option of last resort, to be used only if one of the >>>>> reconstructed # pathway distributions looks like a single curve. >>>>> This is a sign that the # initial guess for the pathway was >>>>> problematic, perhaps due to poor alignment # between the individual and the atlas. >>>>> Setting the reinit parameter to 1 and # rerunning "trac-all -prior" >>>>> and "trac-all -path", only for the specific # subjects and pathways >>>>> that had this problem, will attempt to reconstruct them # with a different initial guess. >>>>> >>>>> # Default: 0 (do not reinitialize) >>>>> >>>>> # >>>>> >>>>> set reinit = 0 >>>>> >>>>> >>>>> >>>>> >>>>> >>>>> >>>>> >>>>> >>>>> >>>> >>>> >>>> The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. >>>> >>>> >>>> >>> >>> >>> >> >> >> >

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Volume ROI statistics
by Garikoitz Lerma-Usabiaga 03 Jul '13

03 Jul '13

Hi, I want to obtain volume statistics from a ROI in fsaverage. I have register.dat and a .nii file (the ROI), but I don't now how to get to the .label. The last try has been: mri_vol2vol --mov $SUBJECTS_DIR/fsaverage/label/ROI.nii --o $SUBJECTS_DIR/fsaverage/label/ROI.mgh --reg $SUBJECTS_DIR/fsaverage/mri/transforms/my_own_register_created_with_bbregister.dat --targ fsaverage (but it is failing, error: corRead(): can't open file $SUBJECTS_DIR/fsaverage/COR-.info) My next steps were: cor2label (in fsaverage) mri_label2label (per every subject) mri_segstats (per every subject) Is there any simpler method to accomplish the same? Or at least that it works? many thanks, Gari

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Re: [Freesurfer] [tracula] running into trouble with nifti data
by Anastasia Yendiki 03 Jul '13

03 Jul '13

Hi Satra - What you're showing in the screenshots would be consistent with a L-R flip of the gradient directions, which is what my comparison of your gradients to the original Siemens gradients indicated. (Assuming the data was acquired with the standard Siemens 60 gradient directions.) a.y On Tue, 2 Jul 2013, Satrajit Ghosh wrote: > hi ay, > i'll look into the orientations a little later tonight, but here is V1 > overlaid on FA for preflip and postflip execution of dtifit. > > cheers, > > satra > > > On Tue, Jul 2, 2013 at 7:48 PM, Anastasia Yendiki > <ayendiki(a)nmr.mgh.harvard.edu> wrote: > > Hi Satra - The orientation of your dwi_orig.nii.gz is LPS. What > flip4fsl will do is convert it to LAS, and perform the same > conversion on the gradient table that you provide. This of > course assumes that dwi_orig.nii.gz and your gradient table are > consistent as they come out of your custom preprocessing. Is > there any chance that they're not? > > Looking at the sample data set that you sent me, I can only see > what came out of your preprocessing and not what went in. I'm > assuming that this is from the standard Siemens 60 direction > sequence, and it has 57 directions b/c 3 of them were thrown out > by your preprocessing. I don't know which directions were thrown > out obviously, but the first 2 directions in your gradient table > are: > -0.99699027939850948 -0.057720153020443053 0.051758263409041924 > 0.63287878858184599 0.066164234845749903 0.77141869630019422 > > The first two directions in the Siemens 60 direction gradient > table are: > 1.0000000000 0.0000000000 0.0000000000 > 0.5867917880 -0.3765825401 -0.7168409782 > > There's not only a small adjustment of the direction but also a > sign change there. So I'd look into that. > > Let me know if any of my assumptions about your original data is > wrong. > > a.y > > On Tue, 2 Jul 2013, Satrajit Ghosh wrote: > > hi anastasia, > i'm trying to debug a seg fault that some folks are > seeing deep into a > tracula run. > > process: > > 1. feed dicoms to a script that runs DTIPrep and > outputs a nifti file, bvec, > bval > > (among checking for many artifacts, this reduces the > b=0 volumes to a single > registered mean volume, runs eddy correction, > discards bad directions, > reorients the gradients appropriately and generates > a report of the quality) > > 2. run tracula on this nifti file > > observation: > > when i run fsl/dtifit on the same nifti file + bvec > + bval, i get the proper > orientation for eigvec1 lines. however, when i feed > this into tracula, the > output of the dtifit step looks terrible - i.e. the > lines are not oriented > as how one might expect water to diffuse. > > suspicion: > > although i have not reached the crash yet, i believe > it might be related to > this fact that the gradients are not oriented > properly relative to the > volume. > > i also suspect that this has something to do with > the flip4fsl step inside > tracula, which is possibly unnecessary because fsl > already likes the input > files. but it seems this step is encoded quite > heavily within the trac-all > scripts. > > questions: > > a. is there a quick way to turn the flip4fsl step > off? > b. alternatively do you have any suggestions for > what to do here? > > the whole point of running through DTIPrep is to > clean up the data before > giving it to tracula or other programs, so i would > really like to keep that > step. > > cheers, > > satra > > > > > > The information in this e-mail is intended only for the person to whom > it is > addressed. If you believe this e-mail was sent to you in error and the > e-mail > contains patient information, please contact the Partners Compliance > HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you > in error > but does not contain patient information, please contact the sender > and properly > dispose of the e-mail. > > > >

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How to change the color of the boundary surface?
by ye tian 03 Jul '13

03 Jul '13

Dear freesurfers, Does anyone know how to change the color (default yellow) of the surface between the grey matter and the white matter? I see green and blue in the tutorial. Thank you very much! Sincerely, Ye

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Re: [Freesurfer] longitudinal analyses for one group
by Sandra Preissler 03 Jul '13

03 Jul '13

Dear Doug, Thanks a lot for your advice. We followed the examples on the wiki (http://ftp.nmr.mgh.harvard.edu/fswiki/FsTutorial/GroupAnalysis#AssembletheD…) and tried to analyze our data as described. We have a single group with two continuous covariates. Therefore we created the freesurfer descriptor file (g1v2_I.fsgd) and three contrast files (main.mtx; age.mtx; Amputationsdauer.mtx). The preprocessing finished without error for both hemispheres. The following command line we used for the preprocessing: mris_preproc --fsgd qdec/GLM/g1v2_I.fsgd --cache-in long.thickness-avg.fwhm10.fsaverage --target fsaverage --hemi lh --out lh.g1v2_I.thickness.10.mgh After this we tried the mri_glmfit for our longitudinal data. Our command line was the following: mri_glmfit --glmdir g1v2_I --y lh.g1v2_I.thickness.10.mgh --fsgd qdec/GLM/g1v2_I.fsgd --C qdec/GLM/main.mtx --C qdec/GLM/age.mtx --C qdec/GLM/Amputationsdauer.mtx We get the error message: ERROR: you must use '--surface subject hemi' with surface data Therfore we tried to specify subject as fsaverage, hemi as lh, surface as thickness. Unfortunately, this did not work out and other alternative approaches to solve this problem did not function as well. Do we have to create a subject descriptor file for our longitudinal templates? Or did we miss anything else? We thought about creating the --X design matrix file for longitudinal data, we found the instruction for between factors. But this could not work for our data. Best regards Sandra -----Nachricht von greve(a)nmr.mgh.harvard.edu -----Datum: Fri, 28 Jun 2013 10:40:57 -0400 Von: Douglas N Greve <greve(a)nmr.mgh.harvard.edu> Betreff: Re: [Freesurfer] longitudinal analyses for one group An: freesurfer(a)nmr.mgh.harvard.edu Hi Sandra, you cannot do the longitudinal statistical analysis in QDEC. You will have to use mri_glmfit. Look at the FSGD examples on the wiki. doug On 06/28/2013 03:28 AM, Sandra Preissler wrote: [Zitattext verstecken] Dear freesurfer experts, we want to do longitudinal analyses for two different time points in only one group (We do not have a control group or different groups within the intervention). So we did the longitudinal processing as described in the tutorial (http://ftp.nmr.mgh.harvard.edu/fswiki/FsTutorial/LongitudinalTutorial#Prepa…) Now we tried to investigate the results with Qdec. We wanted to test whether the averaged change of thickness between pre-test and post-test differs from zero. We had the experience that we could only test the averaged thickness by choosing a discrete factor in Qdec. Our Problem is that we don't have a control group. So we couldn't choose the group variable as discrete factor. It's an pre-post-design with only one group. Is it possible to investigate changes in thickness between pre-test and post-test without choosing a factor? Or do we have to create a group variable as discrete factor? Can you help us in fixing this problem? Thanks in advance! Sandra ---------------------------------------------------------------- This message was sent through https://webmail.uni-jena.de _______________________________________________ Freesurfer mailing list Freesurfer(a)nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer ---------------------------------------------------------------- This message was sent through https://webmail.uni-jena.de

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Re: [Freesurfer] Xhemi files can not be downloaded
by Hamza Alshuft 03 Jul '13

03 Jul '13

Thanks for the help Unfortunately after your reply I could only download ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/mris_preproc the rest of the files are still un-downloadable! I have Mac freesurfer v5.1 and use "curl –O ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/mri_convert" to download the files Any help please! Thanks Hamza ok, try now On 07/02/2013 06:53 AM, Hamza Alshuft wrote: > Dear Freesurfer experts > > I can not download files required for surface based inter hemispheric > registration :http://ftp.nmr.mgh.harvard.edu/fswiki/Xhemi outcome > "curl: (78) RETR response: 550" > I am currently using Mac version 5.1. Any help please? > > > Thanks, > Hamza This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system, you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.

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error in downloading tutorial dataset
by Claudia Dacquino 03 Jul '13

03 Jul '13

Hi freesurfers, I'm trying downloading the tutorial dataset but after several attempts I still couldn't connect to the server and I recived this message repeately, even with the -c flag: "server response error, closing connection". I'm working on Linux Centos System and using the wget command. Thanks everyone. Claudia

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Freesurfer July 2013