Dear FreeSurfer users,
I am using FreeSurfer 6.0 and Tracula 6.0. For many subjects that I'm processing, the volume and path length are equal to each other. For ~60% of subjects, the "pathstats.overall.txt" file shows something like:
Count 1500
Volume 34
Len_Min 34
Len_Max 34
Len_Avg 34
Len_Center 34
AD_Avg 0.00104677
AD_Avg_Weight 0.00104677
AD_Avg_Center 0.00104677
RD_Avg 0.000594649
RD_Avg_Weight 0.000594649
RD_Avg_Center 0.000594649
MD_Avg 0.000745357
MD_Avg_Weight 0.000745357
MD_Avg_Center 0.000745357
FA_Avg 0.364984
FA_Avg_Weight 0.364984
FA_Avg_Center 0.364984
This output suggests that only a single pathway was computed for a subject with output like above (since Len_Min = Len_Max = Len_Avg = Len_Center); yet the count number suggests that 1,500 paths were generated.
This obviously seems problematic. I have two questions:
1) Does the problem originate during the fitting of the ball-and-stick models at each voxel? (I used n=3 sticks with bedpostx). If so, how can I rerun this process to ensure this problem does not repeat?
2) Is it possible that each probabilistic track was generated with the same random seed? If so, would this problem be associated with the ball-and-stick model step (with bedpostx_gpu) or pathway fitting step (-path)?
3) Are the anisotropy measures (such as FA_Avg, MD_Avg_Weight, etc) independent of this problem? Or do they also require different probabilistic paths be generated?
I suspect this issue may have arisen due to my using "bedpostx_gpu" to fit the ball-and-stick models (default setting).
Thank you much in advance.
Best,
Chintan