Freesurfer October 2019

freesurfer@nmr.mgh.harvard.edu

111 participants
180 discussions

Tracula
by Juan Rivas 08 Oct '19

08 Oct '19

External Email - Use Caution Hi, I have been trying to process my diffusion images, but I have a new problem: I wrote this dmrirc: # FreeSurfer SUBJECTS_DIR # T1 images and FreeSurfer segmentations are expected to be found here # setenv SUBJECTS_DIR /autofs/cluster/neuromod/rivas/imagenes/DICOM # Output directory where trac-all results will be saved # Default: Same as SUBJECTS_DIR # set dtroot = /autofs/cluster/neuromod/rivas/imagenes/DICOM/esq-15-hc # Subject IDs # set subjlist = (esq-15-hc) # Default: Run analysis on all subjects # set runlist = (1) # Input diffusion DICOMs (file names relative to dcmroot) # If original DICOMs don't exist, these can be in other image format # but then bvecfile and bvalfile must be specified (see below) # set dcmroot = /autofs/cluster/neuromod/rivas/imagenes/DICOM/esq-15-hc set dcmlist = (dwi.nii.gz) # Diffusion gradient table # Must be specified if inputs are not MGH DICOMs # Three-column format, one row for each volume in the diffusion data set # Default: Read from DICOM header # set bvecfile = /autofs/cluster/neuromod/rivas/imagenes/DICOM/esq-15-hc/processing/31190669_bvecs.dat # Diffusion b-value table # Must be specified if inputs are not MGH DICOMs # Single-column format, one value for each volume in the diffusion data set # Default: Read from DICOM header # set bvalfile = /autofs/cluster/neuromod/rivas/imagenes/DICOM/esq-15-hc/processing/31190669_bvals.dat # Perform registration-based B0-inhomogeneity compensation? # Default: 0 (no) # # set dob0 = 1 # Input B0 field map magnitude DICOMs (file names relative to dcmroot) # Only used if dob0 = 1 # Default: None # # set b0mlist = (huey/fmag/XXX-1.dcm dewey/fmag/XXX-1.dcm louie/fmag/XXX-1.dcm) # Input B0 field map phase DICOMs (file names relative to dcmroot) # Only used if dob0 = 1 # Default: None # # set b0plist = (huey/fphas/XXX-1.dcm dewey/fphas/XXX-1.dcm louie/fphas/XXX-1.dcm) # Echo spacing for field mapping sequence (from sequence printout) # Only used if dob0 = 1 # Default: None # set echospacing = 0.7 # Perform registration-based eddy-current compensation? # Default: 1 (yes) # set doeddy = 1 # Rotate diffusion gradient vectors to match eddy-current compensation? # Only used if doeddy = 1 # Default: 1 (yes) # set dorotbvecs = 1 # Fractional intensity threshold for BET mask extraction from low-b images # This mask is used only if usemaskanat = 0 # Default: 0.3 # set thrbet = 0.5 # Perform diffusion-to-T1 registration by flirt? # Default: 0 (no) # set doregflt = 0 # Perform diffusion-to-T1 registration by bbregister? # Default: 1 (yes) # set doregbbr = 1 # Perform registration of T1 to MNI template? # Default: 1 (yes) # set doregmni = 1 # MNI template # Only used if doregmni = 1 # Default: $FSLDIR/data/standard/MNI152_T1_1mm_brain.nii.gz # set mnitemp = $FSLDIR/data/standard/MNI152_T1_1mm_brain.nii.gz # Perform registration of T1 to CVS template? # Default: 0 (no) # set doregcvs = 0 # CVS template subject ID # Only used if doregcvs = 1 # Default: cvs_avg35 # set cvstemp = donald # Parent directory of the CVS template subject # Only used if doregcvs = 1 # Default: # set cvstempdir = $FREESURFER_HOME/subjects # Use brain mask extracted from T1 image instead of low-b diffusion image? # Has no effect if there is no T1 data # Default: 1 (yes) # set usemaskanat = 1 # Paths to reconstruct # Default: All paths in the atlas # set pathlist = ( lh.cst_AS rh.cst_AS \ lh.unc_AS rh.unc_AS \ lh.ilf_AS rh.ilf_AS \ fmajor_PP fminor_PP \ lh.atr_PP rh.atr_PP \ lh.ccg_PP rh.ccg_PP \ lh.cab_PP rh.cab_PP \ lh.slfp_PP rh.slfp_PP \ lh.slft_PP rh.slft_PP ) # Number of path control points # It can be a single number for all paths or a different number for each of the # paths specified in pathlist # Default: 7 for the forceps major, 6 for the corticospinal tract, # 4 for the angular bundle, and 5 for all other paths # set ncpts = (6 6 5 5 5 5 7 5 5 5 5 5 4 4 5 5 5 5) # List of training subjects # This text file lists the locations of training subject directories # Default: $FREESURFER_HOME/trctrain/trainlist.txt # set trainfile = $FREESURFER_HOME/trctrain/trainlist.txt # Number of "sticks" (anisotropic diffusion compartments) in the bedpostx # ball-and-stick model # Default: 2 # set nstick = 2 # Number of MCMC burn-in iterations # (Path samples drawn initially by MCMC algorithm and discarded) # Default: 200 # set nburnin = 200 # Number of MCMC iterations # (Path samples drawn by MCMC algorithm and used to estimate path distribution) # Default: 7500 # set nsample = 7500 # Frequency with which MCMC path samples are retained for path distribution # Default: 5 (keep every 5th sample) # set nkeep = 5 # Reinitialize path reconstruction? # This is an option of last resort, to be used only if one of the reconstructed # pathway distributions looks like a single curve. This is a sign that the # initial guess for the pathway was problematic, perhaps due to poor alignment # between the individual and the atlas. Setting the reinit parameter to 1 and # rerunning "trac-all -prior" and "trac-all -path", only for the specific # subjects and pathways that had this problem, will attempt to reconstruct them # with a different initial guess. # Default: 0 (do not reinitialize) # set reinit = 0 And I gave this command: [shiraz:processing] (nmr-stable6-env) trac-all -prep -c dmrirc-esq-15-hc But there is an error: INFO: SUBJECTS_DIR is /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing INFO: Diffusion root is /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc Actual FREESURFER_HOME /autofs/cluster/freesurfer/centos7_x86_64/stable6 trac-preproc -c /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/esq-15-hc/scripts/dmrirc.local -log /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/esq-15-hc/scripts/trac-all.log -cmd /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/esq-15-hc/scripts/trac-all.cmd #------------------------------------- /usr/local/freesurfer/stable6/bin/trac-preproc #------------------------------------- #@# Image corrections Tue Oct 8 17:53:12 EDT 2019 mri_convert --bvec-voxel /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/dwi.nii.gz /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/esq-15-hc/dmri/dwi_orig.nii.gz mri_convert.bin --bvec-voxel /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/dwi.nii.gz /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/esq-15-hc/dmri/dwi_orig.nii.gz niiRead(): error opening file /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/dwi.nii.gz $Id: mri_convert.c,v 1.226 2016/02/26 16:15:24 mreuter Exp $ reading from /autofs/cluster/neuromod/rivas/imagenes/Diffusion_Processing/esq-15-hc/dwi.nii.gz... Linux shiraz.nmr.mgh.harvard.edu 3.10.0-957.1.3.el7.x86_64 #1 SMP Thu Nov 29 14:49:43 UTC 2018 x86_64 x86_64 x86_64 GNU/Linux trac-preproc exited with ERRORS at Tue Oct 8 17:53:12 EDT 2019 What may I do? Thanks, JC.

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nu_correct: crashed while running nu_estimate_np_and_em (termination status=512)
by Durgerian, Sally 08 Oct '19

08 Oct '19

External Email - Use Caution Hi all, This post is a follow-up to a question I asked a long time ago. I finally figured out a way around my problem, and wanted to share it with anyone else who is getting this error. I was reading this thread, which referred to problems people were having using virtual machines: https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg45472.html Though not using a virtual machine, I was trying to run freesurfer on data that were housed on a different computer via a mounted partition. The above thread gave me the idea to try copying my data from the mounted partition to the local machine, and when I did that everything ran just fine. Sally

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Re: [Freesurfer] ECC or non-ECC Memory?
by fsbuild 08 Oct '19

08 Oct '19

External Email - Use Caution Hello Sotiris, My understanding is that ECC memory gives you something like a parity check across memory writes/reads.  Or there is some extra bit(s) in hardware that contribute to being able to determine if the value in memory to be read actually matches what was written there.  So for number crunching on Wall Street, suppose there was a RAM failure that manifests itself by resulting in a *wrong* financial calculation that a human being would not detect from monitoring the results.  If that error was from a bit mistakenly flipping in memory, than ECC can help.  ECC can detect a memory error in 2 bits but can only fix 1 bit of whatever is in memory.  Depending upon what bit(s) have mistakenly flipped, it could be a very small or very large error. For this to work, I think the (hardware) memory controller and the CPU need to support ECC as well.  So if the motherboard you are spec'ing out does not explicitly say it supports ECC, then I would assume it does not.  ECC memory is 2X to 3X more expensive than non-ECC memory. I don't know how motherboard prices compare that do/do not support ECC.  Users who are building machines for supper fast gaming, or audio/video production and streaming typically won't spend extra money on ECC as they want to max out on the CPU, memory, and buss/bandwidth. I have seen unsubtle RAM and chip failures cause blatant problems, e.g., the kernel running on an idle server that is on 24/7 all of a sudden starts to panic because of a RAM failure.  I have seen the sign bit mistakenly flip on an ALU chip because it was too close to the heat sink of another chip such that every positive number became negative inside the ALU.  This was obvious after the motherboard heated up (and ECC would not have fixed this).  The BIOS you can run on motherboards have various diagnostics which I think include memory tests, e.g., writing and reading a repeating pattern in/out of memory multiple times to check for consistency.  You could look for what diagnostics are provided in the BIOS program supported by the motherboard you choose. Some RAM manufacturers have fixed warranties or limited lifetime warranties. I've always had good experience with Crucial memory (who make both non-ECC and ECC RAM). As Matt said, you should run some diagnostic test on the RAM when it is new - and then periodically.   Once the warranty expires, you are stuck with any defective RAM no matter why it failed.. I don't think ECC memory is going to have much effect upon how efficiently a program utilizes multiple cores and threads.  I have not compared the clock speeds, but I could imagine ECC memory might be a bit slower than non-ECC memory.  If you have the budget to spec out a machine with a motherboard and CPU you like that supports ECC memory, then I would try it.  It's also good to look online to see who else has already built a machine with the motherboard and CPU you want to use. - R. Glasser, Matthew October 6, 2019 at 16:04         External Email - Use Caution         If you use non-ECC memory, it is a good idea to run something like memtest86 to ensure that it is error free (and return any sticks with errors to the manufacturer).  ECC and non-ECC RAM should be similar assuming the non-ECC RAM doesn’t have errors 😉   Matt.   From: <freesurfer-bounces(a)nmr.mgh.harvard.edu> on behalf of Sotiris Michos <sotmihos(a)gmail.com> Reply-To: Freesurfer support list <freesurfer(a)nmr.mgh.harvard.edu> Date: Sunday, October 6, 2019 at 3:01 PM To: "freesurfer(a)nmr.mgh.harvard.edu" <freesurfer(a)nmr.mgh.harvard.edu> Subject: [Freesurfer] ECC or non-ECC Memory?           External Email - Use Caution         Dear Freesurfer community,   I am interested in building a PC with the goal of running Freesurfer (about 2000-2500 Euros). I have carefully read this very informative webpage. However, I still have some questions and I would be glad if some assistance could be provided.   I suspect that a proper PC would be placed towards the workstation side of the spectrum, since from what I read in the above webpage, Freesurfer can utilize multiple cores and is quite demanding in RAM memory per instance.   What I would like to ask is whether ECC Memory is critical for this application or if the corruption coming from a non-ECC module has any significant effect on the end result. Also, any advice or suggestions about the system's buildup would be more than welcome.   Best Regards, Sotiris Michos   The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. _______________________________________________ Freesurfer mailing list Freesurfer(a)nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer Sotiris Michos October 6, 2019 at 16:01         External Email - Use Caution        Dear Freesurfer community, I am interested in building a PC with the goal of running Freesurfer (about 2000-2500 Euros). I have carefully read this very informative webpage. However, I still have some questions and I would be glad if some assistance could be provided. I suspect that a proper PC would be placed towards the workstation side of the spectrum, since from what I read in the above webpage, Freesurfer can utilize multiple cores and is quite demanding in RAM memory per instance. What I would like to ask is whether ECC Memory is critical for this application or if the corruption coming from a non-ECC module has any significant effect on the end result. Also, any advice or suggestions about the system's buildup would be more than welcome. Best Regards,Sotiris Michos _______________________________________________ Freesurfer mailing list Freesurfer(a)nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer

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Main effect of sex for total surface area, but not for summed vertex-wise surface area
by Linn Christin Bonaventure Norbom 08 Oct '19

08 Oct '19

External Email - Use Caution Hello all Freesurfer experts! Through my analysis I found that, if I plot the "white surface total area" found in subjid/stats/?h.aparc.stats by sex, I as expected find that males have overall larger surface area as compared to females. When plotting the summed verticies of ?h.area for each person on the other hand (albeit after mapping the ?h.area to fsaverage), this main association with sex is not at all present. I am a bit puzzled by this and I am wondering if some sort of global scaling is performed when computing vertex-wise surface area? So that corresponding differences in vertex-wise area are relative sex differences? I understand that area at a single vertex does not hold biological relevance in the same way that for instance thickness does, but unfortunately I still don't quite understand the differing sex associations between aparcs total surface area and the summed vertex-wise surface area. Thanks so much in advance! Kind regards Linn

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Accessing the FS mail archive online
by Adam Martersteck 08 Oct '19

08 Oct '19

External Email - Use Caution Hi Freesurfer team, Just in case this hasn't been brought to your attention -- it looks like the online mail archive requires a martinos center login. Accessing links to past questions and answers via google or just https://mail.nmr.mgh.harvard.edu/pipermail//freesurfer/ prompts for a login. I can replicate this on multiple computers, phones, on and off different VPNs. It's been happening for the last week or two. -Adam

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Read out N from mri_glmfit outputs?
by Caspar M. Schwiedrzik 08 Oct '19

08 Oct '19

External Email - Use Caution Hi! Is there a way to read out the N of a fixed effects analysis performed with mri_glmfit? If not, is it safe to assume that it is (number of volumes*number of runs)-excluded time points, summed across subjects? Thanks! Caspar

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Re: [Freesurfer] ERROR gtmseg
by Marina Fernández 08 Oct '19

08 Oct '19

External Email - Use Caution Dear Douglas, Sorry! This is the previous information: > Dear Douglas, > > Thank you for the replay. > > I think so, I can't find the code 229 in any subject. > > I can't see anything strange in the aparc+aseg.mgz. The cerebral voxels have the normal > > segmentation and labels. Could it be a problem with the extracerebral voxels? > > Best regards, > Marina. > That is kind of weird. It says it has found a segmentation with number > 229, but we don't have that code in our system. Can you find the 229 voxels > in the aparc+aseg.mgz? > Dear experts, > > The following error occurs with one subject when I am running gtmseg > command: > > Computing colortable > ERROR: cannot find match for subcortical segid 229 > ERROR: mri_gtmseg -s qsm_04_year3 --usf 2 --o gtmseg.mgz --apas > apas+head.mgz --no-subseg-wm --no-keep-cc --no-keep-hypo > gtmseg existed with errors > ans=1 > > Do you know what happened with this subject? What can I do to solve it? > > Thank you very much in advance. > Marina.

2 3

negative values of hippocampal subfields volumes
by Krzysztof Gbyl 08 Oct '19

08 Oct '19

External Email - Use Caution Hi all, I am currently running a longitudinal hippocampal subfields analysis using version 6.0. Based on the visual inspection of images, I see that Freesurfer is doing a great job. The problem is that I get negative values of the subfields volumes e.g. Hippocamap_tail -420.400366 subiculum -368.134590 The values seems to be reasonable, but why they are negative, i.e. why they have a '-' sign. Could the minus sign be a hyphen? Has anybody encounther this problem? I hope you can help BW, Chris

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FreeSurfer freezing during processing.
by Троицкий Антон Александ рович 07 Oct '19

07 Oct '19

External Email - Use Caution Hello FreeSurfer Developers, I have installed the FreeSurfer how described here https://surfer.nmr.mgh.harvard.edu/fswiki/WindowsSupport. I launch an Ubuntu terminal and stat a freesurfer application from an Ubuntu command line. [cid:4348455d-9967-4416-9824-c67b83e00ac4@fccho-moscow.ru] I can see in a task manager that different tasks are working and the processing is going. I can see the output in the ubuntu terminal and see the last steps of processing. I don’t know exactly when but after some period of time the processing is freezing and paused. It is really strange because I don’t see any task in the task manager associated with the processing of. It lasts a while and I decide to stop the processing by using ctrl+c. And it is so strange the processing continues after that. It happened twice for the same dataset. I have launched the processing of test data sample-001.nii.gz. It is going well. I have two different datasets that have been processed on the other station and the processing was completed without errors. It is an output in recon-all.cmd file #@# EM Registration Thu Oct 3 09:51:44 MSK 2019 mri_em_register -rusage /usr/local/freesurfer/subjects/2019-07-22_3DBrainView_T1_2019-10-03_09-18/touch/rusage.mri_em_register.dat -uns 3 -mask brainmask.mgz nu.mgz /usr/local/freesurfer/average/RB_all_2016-05-10.vc700.gca transforms/ #@# CA Normalize Thu Oct 3 11:42:10 MSK 2019 mri_ca_normalize -c ctrl_pts.mgz -mask brainmask.mgz nu.mgz /usr/local/freesurfer/average/RB_all_2016-05-10.vc700.gca transforms/talairach.lta norm.mgz #-------------------------------------- #@# CA Reg Thu Oct 3 11:43:49 MSK 2019 mri_ca_register -rusage /usr/local/freesurfer/subjects/2019-07-22_3DBrainView_T1_2019-10-03_09-18/touch/rusage.mri_ca_register.dat -nobigventricles -T transforms/talairach.lta -align-after -mask brainmask.mgz norm.mgz /usr/local/freesu$ Is it normal that the process mri_em_register was going so long? And it continued after I pressed the ctrl+c command. Is it normal this behavior of processing of the data? How do you think what is the cause of this kind of behavior of the processing? Does anybody try to do it? I have found this way for parallel processing. https://andysbrainbook.readthedocs.io/en/latest/FreeSurfer/FS_ShortCourse/F…. But it is not the same. Best regards, Anton Troitskii.

2 1

Question about using tkregisterfv to check registration
by Vicky Yang 07 Oct '19

07 Oct '19

External Email - Use Caution Dear Freesurfer experts, I used mri_coreg to register my pet data to T1 like below: mri_coreg --mov pet.nii.gz --ref T1.nii --reg pet_T1.reg.lta After coregistering, I checked the registration using tkregisterfv like below: tkregisterfv --mov pet.nii.gz --targ T1.nii --reg pet_T1.reg.lta However, the command didn't work with an error: if: Expression Syntax. Any suggestions would be appreciated. Thank you in advance for your help. Sincerely, Vicky

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Freesurfer October 2019