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P files
by John Anderson 06 Jan '16

06 Jan '16

3 2

FDR correction
by stdp82＠virgilio.it 06 Jan '16

06 Jan '16

Hi list, I would like to ask you a number of questions regarding the FDR which is performed following seed-based fcMRI using FS-FAST.1-In the previous mail you have suggested me to use mir_fdr command line (version 6.0). Because I have some problem with new version license (as reported in previous mail) and, overall I have run my data using version 5.3, I would ask you whether FDR integrated in tksurfer (version 5.3) is however reliable as the FDR included in 6.0? 2-Becouse the FDR analysis was performed separately for the right and left hemispheres and the subcortical structures, should I set the FDR threshold to 0.05/3 in order to correct for multiple comparison? (I have one group) 3- Is easy for me to follow the guidelines and visualize the cortical areas which are functionally connected with the omolateral seed. How can I do to visualize also the the cortical areas which are functionally connected with the controlateral seed? If change only the rh/lh in command line the visualization of the results is wrong. 4- I'd like to perform mri_surfcluster on a group to eliminate cluster < 5 voxels from sig.nii.gz after FDR correction. I'm running mri_surfcluster --in sig.nii.gz --hemi rh --sign abs --fdr 0.05 --minarea 5 --sum summaryfile --ocn ocnid but --subject ... must be included.How should do I do? I would like to run my analysis on group results, not on a single subject. Thanks for your work.Regards, Stefano

2 1

.w file for freeview
by peng 06 Jan '16

06 Jan '16

Hi Freesurfers, I tried to overlay .w file onto a surface, and failed. I dug the previous posts and know this file format is obsolete now. However I wish to know in which version of freeview can I still use this file format, if possible. It will be great if you can provide the link for it. If this is not possible, is there a matlab tool similar to 'write_file.m' so I can output my data in matlab to the newer format (substitution of w-file)? Thanks in advance. best Peng

3 5

Recon-all Run Time
by Marissa Pifer 06 Jan '16

06 Jan '16

Hi Freesurfers, I've recently downloaded free surfer onto a new computer. It's working correctly, but the running time for the recon-all -all command is 30-50 hours. This is in contrast to when I've used free surfer on previous computers where the run time was 6-12 hours. I'm wondering why this would be the case and if anyone else has run into this problem. Marissa

2 2

Need help finding minimum number of vertices for monte carlo correction
by Tara Miskovich 06 Jan '16

06 Jan '16

Hello Freesurfer experts, I need help me finding how to get the minimum number of vertices required to pass the cluster-corrected threshold with monte carlo simulations used in qdec for a surface based analysis? This is per a reviewer request, but I cannot find anything on the wiki or in the mail archives on how to get this value. Can someone please help me with this issue? -- Thank you, Tara

2 6

output from mri_glmfit
by Peggy Skelly 06 Jan '16

06 Jan '16

Hello Freesurfer statistical experts, I need some assistance in explaining the output generated by mri_glmfit (then followed by correction for multiple comparisions, mri_glmfit-sim). We fit one model and got some statistically significant clusters. Then, we ran another model, the same plus an additional nuisance variable. It had similar significant clusters. How do we compare the results from the 2 different models? Which model is better? In other statistic programs, we could examine an R^2 value, which can be interpreted as the percent of the response variable variation that is explained by the model. The model with the higher R^2 would be considered better since it fits the data better. But the output from mri_glmfit has rvar.mgh - the sum of the squares of the residual error divided by the DOF. So it would seem that a lower rvar would indicate a better fit. Can rvar.mgh be used to compute an R^2, because the R^2 concept is more widely understood. Or, is there a way to explain rvar in lay terms similar to R^2? Thanks, Peggy

2 1

how to display individual Desikan parcels?
by Mihaela Stefan 05 Jan '16

05 Jan '16

Hello Freesurfers, I would like to be able to display just a few parcels from the Desikan atlas on the fsaverage inflated surface. For example, I would like to display only the superiorfrontal and the posteriorcingulate. I tried to recreate the parcels myself by drawing labels, but they don't look pretty (see the attachment). I traced the labels in qdec (based on the Annotation/Opacity tab) and then I mapped them to fsaverage and visualized them with freeview. There's probably a better way to do that but I couldn't figure it out. Can anyone help me with this? Thanks! Mihaela

2 6

.Img format
by Hassan bakhshi 05 Jan '16

05 Jan '16

Hello , I have an image with .img format and an image with .hdr format. Freesurfer cant run them. What im suppose to di0o??? I just want to view the image.

2 1

mri_watershed for infants or NHP
by Mahmoud 05 Jan '16

05 Jan '16

Dear experts, I'm trying to adopt the HCP pipeline to our own data but at it is originally developed for human adults it fails frequently on my data. A big chunk of the pipeline is based on FreeSurfer so I thought it should be most relevant to ask my question here. the main problem I have here is that mri_watershed fails so the rest of the pipeline cannot proceed. The errors are usually like below: ****************TEMPLATE DEFORMATION**************** second estimation of the COG coord: x=128,y=131, z=124, r=39161 iterations ^^^^^^^^ couldn't find WM with original limits - expanding ^^^^^^ GLOBAL CSF_MIN=1, CSF_intensity=2, CSF_MAX=3 , nb = 38180 RIGHT_CER CSF_MIN=1, CSF_intensity=2, CSF_MAX=3 , nb = -1043712479 LEFT_CER CSF_MIN=1, CSF_intensity=2, CSF_MAX=3 , nb = 1117744481 RIGHT_BRAIN CSF_MIN=1, CSF_intensity=2, CSF_MAX=3 , nb = -1064196139 LEFT_BRAIN CSF_MIN=1, CSF_intensity=2, CSF_MAX=3 , nb = 1050954328 OTHER CSF_MIN=1, CSF_intensity=2, CSF_MAX=3 , nb = 1062732290 mri_watershed Error: GLOBAL region of the brain empty ! ################################################################## or *************************WATERSHED************************** Sorting... mri_watershed Error: Problem in the COG calculation: T1.mgz may not contain properly normalized white-matter. Confirm that T1.mgz has enough white-matter voxels of value 110. If white-matter contains few if any voxels of value 110, try adding wm control-points to nu.mgz, and re-run recon-all. ################################################################# I really appreciate any help or direction. Thank you! Mahmoud

1 0

Re: [Freesurfer] Individual regions of CorticalParcellation_Yeo2011
by Parker, Richard 05 Jan '16

05 Jan '16

Hi Professor Yeo, Sorry for the late reply. In the end I ended up giving in and performing my functional connectivity analysis in MNI space, like any rational person would. As a result, I ended up using the MNI152 versions of network masks you provided. Thanks for all your help, and for providing the split region ROI files! Best wishes, Richard Parker Richard Parker Postgraduate Research Student Maurice Wohl Clinical Neuroscience Institute King's College London Telephone: 07943515208 Email: Richard.r.parker(a)kcl.ac.uk

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