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Looks like your setting for SUBJECTS_DIR together with the last 2 command line arguments, tester  /mri/norm.mgz - is causing it to look for the path $SUBJECTS_DIR/tester/mri/aseg.mgz - which does not exist.   Or I don’t see a directory $SUBJECTS_DIR/tester in the 7.3.2 release to read from and you cannot by default write to or create a new subdirectory ./tester under $SUBJECTS_DIR Did you mean to set  SUBJECTS_DIR to something different where $SUBEJCTS_DIR/tester/mri/aseg.mgz already exists?  Maybe you need to change your command line arguments to be different. - R. On Oct 12, 2022, at 22:25, priya padma <priya@phenomx.ai> wrote:        External Email - Use Caution        Hello Team, I am trying to find the volume of WM, GM, CSF separately and I came across this command namely"mri_compute_volume_fractions"Looking through all the email archives I gave the following line as the command after setting the paths$ mri_compute_volume_fractions --o test --regheader tester /mri/norm.mgzbut I get the following erroruser-MacBook-Pro:sub-AD003S1059S61129 user$ mri_compute_volume_fractions --o test --regheader tester /mri/norm.mgz supposed to be reproducible but seed not set7.3.2sysname  Darwinhostname user-MacBook-Pro.localmachine  x86_64user     <username>setenv SUBJECTS_DIR /Applications/freesurfer/7.3.2/subjectscd /Applications/freesurfer/7.3.2/subjects/sub-AD003S1059S61129mri_compute_volume_fractions --o test --regheader tester /mri/norm.mgz outstem testregfile (null)regtype 0segfile aseg.mgzwsurf whitepsurf pialTempVolFile /mri/norm.mgzUSF 2Reading in aseg and surfs from /Applications/freesurfer/7.3.2/subjects/testerLoading /Applications/freesurfer/7.3.2/subjects/tester/mri/aseg.mgzerror: mghRead(/Applications/freesurfer/7.3.2/subjects/tester/mri/aseg.mgz, -1): could not open fileI set all the paths so why am I not able to read the mgz file?Could anyone help me with this ?Thank you -- Best RegardsPadma PriyaAI Developer PhenoMx, Inc.priya@phenomx.ai | (+82)10-5405-2685_______________________________________________Freesurfer mailing listFreesurfer@nmr.mgh.harvard.eduhttps://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer%E2%80%8B