External Email - Use Caution
Hello,
I am trying to make a study specific template based on the instructions at: https://surfer.nmr.mgh.harvard.edu/fswiki/SurfaceRegAndTemplates
However, on running the first step: make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ...
I get an error while running the recon-all is trying to run the aparc to aseg step:
#@# AParc-to-ASeg aparc Thu Dec 5 11:29:33 EST 2019
/Users/nandita/FreeSurfer_recon/newtemplate
\n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos --relabel mri/norm.mgz mri/transforms/talairach.m3z /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca mri/aseg.auto_noCCseg.label_intensities.txt \n
relabeling unlikely voxels interior to white matter surface:
norm: mri/norm.mgz
XFORM: mri/transforms/talairach.m3z
GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca
label intensities: mri/aseg.auto_noCCseg.label_intensities.txt
SUBJECTS_DIR /Users/nandita/FreeSurfer_recon
subject newtemplate
outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz
useribbon 0
baseoffset 0
RipUnknown 0
Reading lh white surface
/Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white
Reading lh pial surface
/Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial
Loading lh annotations from /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot
reading colortable from annotation file...
colortable with 36 entries read (originally /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab)
Reading rh white surface
/Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white
Reading rh pial surface
/Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial
Loading rh annotations from /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot
reading colortable from annotation file...
colortable with 36 entries read (originally /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab)
Have color table for lh white annotation
Have color table for rh white annotation
Loading ribbon segmentation from /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz
Building hash of lh white
Building hash of lh pial
Building hash of rh white
Building hash of rh pial
Loading aseg from /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz
ASeg Vox2RAS: -----------
-1.00000 0.00000 0.00000 128.00000;
0.00000 0.00000 1.00000 -128.00000;
0.00000 -1.00000 0.00000 128.00000;
0.00000 0.00000 0.00000 1.00000;
mghRead(mri/norm.mgz, -1): could not open file
-------------------------
Labeling Slice
relabeling unlikely voxels in interior of white matter
Segmentation fault
Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: Sun Jun 2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64
recon-all -s newtemplate exited with ERRORS at Thu Dec 5 11:29:52 EST 2019
On inspecting the mri folder, it seems the norm.mgz file has not been created at all. Looking at the flowchart of steps for the recon-all command, norm.mgz is supposed to be created in the CA Normalize step, which requires the talairach.lta in the newtemplate/mri/transforms folder, which seems to be missing as well. The only transform created in the transform folder is talairach.xfm. The talairach.lta is created in the EM GCA registration step. Do I re-run the EM GCA registration, CA normalize, and then contonue from aparc to aseg? I am not sure if since I am creating my own template, the EM GCA step requires any modifications? Any help will be appreciated.
Thanks, Nandita
See the REAME file here ftp://surfer.nmr.mgh.harvard.edu/pub/dist/freesurfer/6.0.0-patch/
On 12/5/19 11:47 AM, Joshi, Nandita wrote:
External Email - Use Caution
Hello,
I am trying to make a study specific template based on the instructions at: https://surfer.nmr.mgh.harvard.edu/fswiki/SurfaceRegAndTemplates
However, on running the first step:
make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ...
I get an error while running the recon-all is trying to run the aparc to aseg step:
#@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019
/Users/nandita/FreeSurfer_recon/newtemplate
\n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos --relabel mri/norm.mgz mri/transforms/talairach.m3z /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca mri/aseg.auto_noCCseg.label_intensities.txt \n
relabeling unlikely voxels interior to white matter surface:
norm: mri/norm.mgz
XFORM: mri/transforms/talairach.m3z
GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca
label intensities: mri/aseg.auto_noCCseg.label_intensities.txt
SUBJECTS_DIR /Users/nandita/FreeSurfer_recon
subject newtemplate
outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz
useribbon 0
baseoffset 0
RipUnknown 0
Reading lh white surface
/Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white
Reading lh pial surface
/Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial
Loading lh annotations from /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot
reading colortable from annotation file...
colortable with 36 entries read (originally /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab)
Reading rh white surface
/Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white
Reading rh pial surface
/Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial
Loading rh annotations from /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot
reading colortable from annotation file...
colortable with 36 entries read (originally /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab)
Have color table for lh white annotation
Have color table for rh white annotation
Loading ribbon segmentation from /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz
Building hash of lh white
Building hash of lh pial
Building hash of rh white
Building hash of rh pial
Loading aseg from /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz
ASeg Vox2RAS: -----------
-1.00000 0.00000 0.00000 128.00000;
0.00000 0.00000 1.00000-128.00000;
0.00000-1.00000 0.00000 128.00000;
0.00000 0.00000 0.00000 1.00000;
mghRead(mri/norm.mgz, -1): could not open file
Labeling Slice
relabeling unlikely voxels in interior of white matter
Segmentation fault
Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64
recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019
On inspecting the mri folder, it seems the norm.mgz file has not been created at all. Looking at the flowchart of steps for the recon-all command, norm.mgz is supposed to be created in the CA Normalize step, which requires the talairach.lta in the newtemplate/mri/transforms folder, which seems to be missing as well. The only transform created in the transform folder is talairach.xfm. The talairach.lta is created in the EM GCA registration step. Do I re-run the EM GCA registration, CA normalize, and then contonue from aparc to aseg? I am not sure if since I am creating my own template, the EM GCA step requires any modifications? Any help will be appreciated.
Thanks,
Nandita
Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
External Email - Use Caution
Thank you so much for that! The README file instructs to download make_average_subject and platform specific mri_aparc2aseg. However, the mac version of mri_aparc2aseg is missing in the patch. Could it be possible to download it from elsewhere?
- Nandita
On 12/5/19, 12:49 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote:
USE CAUTION: External Message.
See the REAME file here https://urldefense.proofpoint.com/v2/url?u=ftp-3A__surfer.nmr.mgh.harvard.ed...
On 12/5/19 11:47 AM, Joshi, Nandita wrote: > > External Email - Use Caution > > Hello, > > I am trying to make a study specific template based on the > instructions at: > https://urldefense.proofpoint.com/v2/url?u=https-3A__surfer.nmr.mgh.harvard.... > > However, on running the first step: > > make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ... > > I get an error while running the recon-all is trying to run the aparc > to aseg step: > > #@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019 > > /Users/nandita/FreeSurfer_recon/newtemplate > > \n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos > --relabel mri/norm.mgz mri/transforms/talairach.m3z > /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > mri/aseg.auto_noCCseg.label_intensities.txt \n > > relabeling unlikely voxels interior to white matter surface: > > norm: mri/norm.mgz > > XFORM: mri/transforms/talairach.m3z > > GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > label intensities: mri/aseg.auto_noCCseg.label_intensities.txt > > SUBJECTS_DIR /Users/nandita/FreeSurfer_recon > > subject newtemplate > > outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz > > useribbon 0 > > baseoffset 0 > > RipUnknown 0 > > Reading lh white surface > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white > > Reading lh pial surface > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial > > Loading lh annotations from > /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot > > reading colortable from annotation file... > > colortable with 36 entries read (originally > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab) > > Reading rh white surface > > /Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white > > Reading rh pial surface > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial > > Loading rh annotations from > /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot > > reading colortable from annotation file... > > colortable with 36 entries read (originally > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab) > > Have color table for lh white annotation > > Have color table for rh white annotation > > Loading ribbon segmentation from > /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz > > Building hash of lh white > > Building hash of lh pial > > Building hash of rh white > > Building hash of rh pial > > Loading aseg from > /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz > > ASeg Vox2RAS: ----------- > > -1.00000 0.00000 0.00000 128.00000; > > 0.00000 0.00000 1.00000-128.00000; > > 0.00000-1.00000 0.00000 128.00000; > > 0.00000 0.00000 0.00000 1.00000; > > mghRead(mri/norm.mgz, -1): could not open file > > ------------------------- > > Labeling Slice > > relabeling unlikely voxels in interior of white matter > > Segmentation fault > > Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: > Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64 > > recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019 > > On inspecting the mri folder, it seems the norm.mgz file has not been > created at all. Looking at the flowchart of steps for the recon-all > command, norm.mgz is supposed to be created in the CA Normalize step, > which requires the talairach.lta in the newtemplate/mri/transforms > folder, which seems to be missing as well. The only transform created > in the transform folder is talairach.xfm. The talairach.lta is created > in the EM GCA registration step. Do I re-run the EM GCA registration, > CA normalize, and then contonue from aparc to aseg? I am not sure if > since I am creating my own template, the EM GCA step requires any > modifications? Any help will be appreciated. > > Thanks, > > Nandita > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed...
_______________________________________________ Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed...
We currently do not have a mac build of mri_aparc2aseg, and we recommend downloading the freesurfer dev release to resolve this issue.
On 12/5/19 1:00 PM, Joshi, Nandita wrote:
External Email - Use CautionThank you so much for that! The README file instructs to download make_average_subject and platform specific mri_aparc2aseg. However, the mac version of mri_aparc2aseg is missing in the patch. Could it be possible to download it from elsewhere?
- Nandita
On 12/5/19, 12:49 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote:
USE CAUTION: External Message. See the REAME file here https://urldefense.proofpoint.com/v2/url?u=ftp-3A__surfer.nmr.mgh.harvard.edu_pub_dist_freesurfer_6.0.0-2Dpatch_&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=6p4504imNdnEktx-WXKqAlxMvMCQ1EYnJW_deB2CAWI&e= On 12/5/19 11:47 AM, Joshi, Nandita wrote: > > External Email - Use Caution > > Hello, > > I am trying to make a study specific template based on the > instructions at: > https://urldefense.proofpoint.com/v2/url?u=https-3A__surfer.nmr.mgh.harvard.edu_fswiki_SurfaceRegAndTemplates&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=LmIaVqBLyDekwkZsc46m3nOdn25B7E96m_UuRzDu3Ew&e= > > However, on running the first step: > > make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ... > > I get an error while running the recon-all is trying to run the aparc > to aseg step: > > #@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019 > > /Users/nandita/FreeSurfer_recon/newtemplate > > \n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos > --relabel mri/norm.mgz mri/transforms/talairach.m3z > /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > mri/aseg.auto_noCCseg.label_intensities.txt \n > > relabeling unlikely voxels interior to white matter surface: > > norm: mri/norm.mgz > > XFORM: mri/transforms/talairach.m3z > > GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > label intensities: mri/aseg.auto_noCCseg.label_intensities.txt > > SUBJECTS_DIR /Users/nandita/FreeSurfer_recon > > subject newtemplate > > outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz > > useribbon 0 > > baseoffset 0 > > RipUnknown 0 > > Reading lh white surface > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white > > Reading lh pial surface > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial > > Loading lh annotations from > /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot > > reading colortable from annotation file... > > colortable with 36 entries read (originally > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab) > > Reading rh white surface > > /Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white > > Reading rh pial surface > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial > > Loading rh annotations from > /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot > > reading colortable from annotation file... > > colortable with 36 entries read (originally > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab) > > Have color table for lh white annotation > > Have color table for rh white annotation > > Loading ribbon segmentation from > /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz > > Building hash of lh white > > Building hash of lh pial > > Building hash of rh white > > Building hash of rh pial > > Loading aseg from > /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz > > ASeg Vox2RAS: ----------- > > -1.00000 0.00000 0.00000 128.00000; > > 0.00000 0.00000 1.00000-128.00000; > > 0.00000-1.00000 0.00000 128.00000; > > 0.00000 0.00000 0.00000 1.00000; > > mghRead(mri/norm.mgz, -1): could not open file > > ------------------------- > > Labeling Slice > > relabeling unlikely voxels in interior of white matter > > Segmentation fault > > Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: > Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64 > > recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019 > > On inspecting the mri folder, it seems the norm.mgz file has not been > created at all. Looking at the flowchart of steps for the recon-all > command, norm.mgz is supposed to be created in the CA Normalize step, > which requires the talairach.lta in the newtemplate/mri/transforms > folder, which seems to be missing as well. The only transform created > in the transform folder is talairach.xfm. The talairach.lta is created > in the EM GCA registration step. Do I re-run the EM GCA registration, > CA normalize, and then contonue from aparc to aseg? I am not sure if > since I am creating my own template, the EM GCA step requires any > modifications? Any help will be appreciated. > > Thanks, > > Nandita > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_mailman_listinfo_freesurfer&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=zJ1S7JojJH4eJNf5L992h-7M4p0030LaYiT6ljZ4lBQ&e= _______________________________________________ Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_mailman_listinfo_freesurfer&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=zJ1S7JojJH4eJNf5L992h-7M4p0030LaYiT6ljZ4lBQ&e=
Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
External Email - Use Caution
Hi Dr. Greve,
Thanks so much for your response. The primary reason for my wanting to do this is due to a large number of outliers in terms of area and volume. Our subject population is cognitively impaired older adults > 60 yrs. The custom surface based template might help in investigating the area outliers, but it wouldn't help in investigating the volume outliers. Since the DK atlas is constructed from a population 18-86 years including 10 people with Alzheimer's Disease, I am not even sure if making a new volume atlas would help.
Attached are graphs showing the distribution of the region wise area, volume, and thickness of all subjects in lh (filled circles) and rh (open circles). I looked into the aparc and aseg labels in specific regions for the subjects that are outliers, but nothing seems to have gone horribly wrong in terms of parcellation. Any pointers on how I can investigate and if a custom volume template might help?
Thanks, Nandita
On 12/5/19, 1:36 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote:
We currently do not have a mac build of mri_aparc2aseg, and we recommend downloading the freesurfer dev release to resolve this issue.
On 12/5/19 1:00 PM, Joshi, Nandita wrote: > External Email - Use Caution > > Thank you so much for that! The README file instructs to download make_average_subject and platform specific mri_aparc2aseg. However, the mac version of mri_aparc2aseg is missing in the patch. Could it be possible to download it from elsewhere? > > - Nandita > > > > On 12/5/19, 12:49 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote: > > USE CAUTION: External Message. > > See the REAME file here > https://urldefense.proofpoint.com/v2/url?u=ftp-3A__surfer.nmr.mgh.harvard.ed... > > > On 12/5/19 11:47 AM, Joshi, Nandita wrote: > > > > External Email - Use Caution > > > > Hello, > > > > I am trying to make a study specific template based on the > > instructions at: > > https://urldefense.proofpoint.com/v2/url?u=https-3A__surfer.nmr.mgh.harvard.... > > > > However, on running the first step: > > > > make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ... > > > > I get an error while running the recon-all is trying to run the aparc > > to aseg step: > > > > #@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019 > > > > /Users/nandita/FreeSurfer_recon/newtemplate > > > > \n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos > > --relabel mri/norm.mgz mri/transforms/talairach.m3z > > /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > mri/aseg.auto_noCCseg.label_intensities.txt \n > > > > relabeling unlikely voxels interior to white matter surface: > > > > norm: mri/norm.mgz > > > > XFORM: mri/transforms/talairach.m3z > > > > GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > > > label intensities: mri/aseg.auto_noCCseg.label_intensities.txt > > > > SUBJECTS_DIR /Users/nandita/FreeSurfer_recon > > > > subject newtemplate > > > > outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz > > > > useribbon 0 > > > > baseoffset 0 > > > > RipUnknown 0 > > > > Reading lh white surface > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white > > > > Reading lh pial surface > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial > > > > Loading lh annotations from > > /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot > > > > reading colortable from annotation file... > > > > colortable with 36 entries read (originally > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab) > > > > Reading rh white surface > > > > /Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white > > > > Reading rh pial surface > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial > > > > Loading rh annotations from > > /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot > > > > reading colortable from annotation file... > > > > colortable with 36 entries read (originally > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab) > > > > Have color table for lh white annotation > > > > Have color table for rh white annotation > > > > Loading ribbon segmentation from > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz > > > > Building hash of lh white > > > > Building hash of lh pial > > > > Building hash of rh white > > > > Building hash of rh pial > > > > Loading aseg from > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz > > > > ASeg Vox2RAS: ----------- > > > > -1.00000 0.00000 0.00000 128.00000; > > > > 0.00000 0.00000 1.00000-128.00000; > > > > 0.00000-1.00000 0.00000 128.00000; > > > > 0.00000 0.00000 0.00000 1.00000; > > > > mghRead(mri/norm.mgz, -1): could not open file > > > > ------------------------- > > > > Labeling Slice > > > > relabeling unlikely voxels in interior of white matter > > > > Segmentation fault > > > > Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: > > Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64 > > > > recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019 > > > > On inspecting the mri folder, it seems the norm.mgz file has not been > > created at all. Looking at the flowchart of steps for the recon-all > > command, norm.mgz is supposed to be created in the CA Normalize step, > > which requires the talairach.lta in the newtemplate/mri/transforms > > folder, which seems to be missing as well. The only transform created > > in the transform folder is talairach.xfm. The talairach.lta is created > > in the EM GCA registration step. Do I re-run the EM GCA registration, > > CA normalize, and then contonue from aparc to aseg? I am not sure if > > since I am creating my own template, the EM GCA step requires any > > modifications? Any help will be appreciated. > > > > Thanks, > > > > Nandita > > > > > > _______________________________________________ > > Freesurfer mailing list > > Freesurfer@nmr.mgh.harvard.edu > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed...
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When you say that you looked at the labels/segmentations and that nothing looked horribly wrong, would that mean that the labels are accurate and that they are not really outliers?
On 12/5/19 7:26 PM, Joshi, Nandita wrote:
External Email - Use CautionHi Dr. Greve,
Thanks so much for your response. The primary reason for my wanting to do this is due to a large number of outliers in terms of area and volume. Our subject population is cognitively impaired older adults > 60 yrs. The custom surface based template might help in investigating the area outliers, but it wouldn't help in investigating the volume outliers. Since the DK atlas is constructed from a population 18-86 years including 10 people with Alzheimer's Disease, I am not even sure if making a new volume atlas would help.
Attached are graphs showing the distribution of the region wise area, volume, and thickness of all subjects in lh (filled circles) and rh (open circles). I looked into the aparc and aseg labels in specific regions for the subjects that are outliers, but nothing seems to have gone horribly wrong in terms of parcellation. Any pointers on how I can investigate and if a custom volume template might help?
Thanks, Nandita
On 12/5/19, 1:36 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote:
We currently do not have a mac build of mri_aparc2aseg, and we recommend downloading the freesurfer dev release to resolve this issue. On 12/5/19 1:00 PM, Joshi, Nandita wrote: > External Email - Use Caution > > Thank you so much for that! The README file instructs to download make_average_subject and platform specific mri_aparc2aseg. However, the mac version of mri_aparc2aseg is missing in the patch. Could it be possible to download it from elsewhere? > > - Nandita > > > > On 12/5/19, 12:49 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote: > > USE CAUTION: External Message. > > See the REAME file here > https://urldefense.proofpoint.com/v2/url?u=ftp-3A__surfer.nmr.mgh.harvard.edu_pub_dist_freesurfer_6.0.0-2Dpatch_&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=6p4504imNdnEktx-WXKqAlxMvMCQ1EYnJW_deB2CAWI&e= > > > On 12/5/19 11:47 AM, Joshi, Nandita wrote: > > > > External Email - Use Caution > > > > Hello, > > > > I am trying to make a study specific template based on the > > instructions at: > > https://urldefense.proofpoint.com/v2/url?u=https-3A__surfer.nmr.mgh.harvard.edu_fswiki_SurfaceRegAndTemplates&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=LmIaVqBLyDekwkZsc46m3nOdn25B7E96m_UuRzDu3Ew&e= > > > > However, on running the first step: > > > > make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ... > > > > I get an error while running the recon-all is trying to run the aparc > > to aseg step: > > > > #@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019 > > > > /Users/nandita/FreeSurfer_recon/newtemplate > > > > \n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos > > --relabel mri/norm.mgz mri/transforms/talairach.m3z > > /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > mri/aseg.auto_noCCseg.label_intensities.txt \n > > > > relabeling unlikely voxels interior to white matter surface: > > > > norm: mri/norm.mgz > > > > XFORM: mri/transforms/talairach.m3z > > > > GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > > > label intensities: mri/aseg.auto_noCCseg.label_intensities.txt > > > > SUBJECTS_DIR /Users/nandita/FreeSurfer_recon > > > > subject newtemplate > > > > outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz > > > > useribbon 0 > > > > baseoffset 0 > > > > RipUnknown 0 > > > > Reading lh white surface > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white > > > > Reading lh pial surface > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial > > > > Loading lh annotations from > > /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot > > > > reading colortable from annotation file... > > > > colortable with 36 entries read (originally > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab) > > > > Reading rh white surface > > > > /Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white > > > > Reading rh pial surface > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial > > > > Loading rh annotations from > > /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot > > > > reading colortable from annotation file... > > > > colortable with 36 entries read (originally > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab) > > > > Have color table for lh white annotation > > > > Have color table for rh white annotation > > > > Loading ribbon segmentation from > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz > > > > Building hash of lh white > > > > Building hash of lh pial > > > > Building hash of rh white > > > > Building hash of rh pial > > > > Loading aseg from > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz > > > > ASeg Vox2RAS: ----------- > > > > -1.00000 0.00000 0.00000 128.00000; > > > > 0.00000 0.00000 1.00000-128.00000; > > > > 0.00000-1.00000 0.00000 128.00000; > > > > 0.00000 0.00000 0.00000 1.00000; > > > > mghRead(mri/norm.mgz, -1): could not open file > > > > ------------------------- > > > > Labeling Slice > > > > relabeling unlikely voxels in interior of white matter > > > > Segmentation fault > > > > Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: > > Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64 > > > > recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019 > > > > On inspecting the mri folder, it seems the norm.mgz file has not been > > created at all. Looking at the flowchart of steps for the recon-all > > command, norm.mgz is supposed to be created in the CA Normalize step, > > which requires the talairach.lta in the newtemplate/mri/transforms > > folder, which seems to be missing as well. The only transform created > > in the transform folder is talairach.xfm. The talairach.lta is created > > in the EM GCA registration step. Do I re-run the EM GCA registration, > > CA normalize, and then contonue from aparc to aseg? I am not sure if > > since I am creating my own template, the EM GCA step requires any > > modifications? Any help will be appreciated. > > > > Thanks, > > > > Nandita > > > > > > _______________________________________________ > > Freesurfer mailing list > > Freesurfer@nmr.mgh.harvard.edu > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_mailman_listinfo_freesurfer&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=zJ1S7JojJH4eJNf5L992h-7M4p0030LaYiT6ljZ4lBQ&e= > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_mailman_listinfo_freesurfer&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=HRcVNlmwdK9YHH6teQ4oW9oc6SDFzz79TS63mOlbWIY&s=zJ1S7JojJH4eJNf5L992h-7M4p0030LaYiT6ljZ4lBQ&e= > > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_mailman_listinfo_freesurfer&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=wvTVN6V23k839lg_BNjhpvOJPbzoBSkJo0AXLRh3e7c&s=Oy4G_oUCj9eqihaPfelNsoQgwOcvmI3qYaBuN4CEmWE&e= _______________________________________________ Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.edu_mailman_listinfo_freesurfer&d=DwIGaQ&c=shNJtf5dKgNcPZ6Yh64b-A&r=Bk3PG21nu20V-TX_S982zJE-KkrgCuPol41Dhbe_0mI&m=wvTVN6V23k839lg_BNjhpvOJPbzoBSkJo0AXLRh3e7c&s=Oy4G_oUCj9eqihaPfelNsoQgwOcvmI3qYaBuN4CEmWE&e=
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Hi Dr. Greve,
I apologize, maybe I should have been more specific. What I meant to say was, for example, in the attached area and volume distribution graphs over regions, we have most outliers in the superior frontal, and inferior parietal regions. However, on examining these regions for the subjects that are outliers, in comparison to the other subjects that are not, there doesn't seem to be an increased inclusion of voxels in these regions which could possibly explain why these might be outliers compared to the rest of the group.
- Nandita
On 12/12/19, 7:02 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote:
When you say that you looked at the labels/segmentations and that nothing looked horribly wrong, would that mean that the labels are accurate and that they are not really outliers?
On 12/5/19 7:26 PM, Joshi, Nandita wrote: > External Email - Use Caution > > Hi Dr. Greve, > > Thanks so much for your response. The primary reason for my wanting to do this is due to a large number of outliers in terms of area and volume. Our subject population is cognitively impaired older adults > 60 yrs. The custom surface based template might help in investigating the area outliers, but it wouldn't help in investigating the volume outliers. Since the DK atlas is constructed from a population 18-86 years including 10 people with Alzheimer's Disease, I am not even sure if making a new volume atlas would help. > > Attached are graphs showing the distribution of the region wise area, volume, and thickness of all subjects in lh (filled circles) and rh (open circles). I looked into the aparc and aseg labels in specific regions for the subjects that are outliers, but nothing seems to have gone horribly wrong in terms of parcellation. Any pointers on how I can investigate and if a custom volume template might help? > > Thanks, > Nandita > > > > On 12/5/19, 1:36 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote: > > We currently do not have a mac build of mri_aparc2aseg, and we recommend > downloading the freesurfer dev release to resolve this issue. > > > > On 12/5/19 1:00 PM, Joshi, Nandita wrote: > > External Email - Use Caution > > > > Thank you so much for that! The README file instructs to download make_average_subject and platform specific mri_aparc2aseg. However, the mac version of mri_aparc2aseg is missing in the patch. Could it be possible to download it from elsewhere? > > > > - Nandita > > > > > > > > On 12/5/19, 12:49 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D." <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu> wrote: > > > > USE CAUTION: External Message. > > > > See the REAME file here > > https://urldefense.proofpoint.com/v2/url?u=ftp-3A__surfer.nmr.mgh.harvard.ed... > > > > > > On 12/5/19 11:47 AM, Joshi, Nandita wrote: > > > > > > External Email - Use Caution > > > > > > Hello, > > > > > > I am trying to make a study specific template based on the > > > instructions at: > > > https://urldefense.proofpoint.com/v2/url?u=https-3A__surfer.nmr.mgh.harvard.... > > > > > > However, on running the first step: > > > > > > make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ... > > > > > > I get an error while running the recon-all is trying to run the aparc > > > to aseg step: > > > > > > #@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019 > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate > > > > > > \n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos > > > --relabel mri/norm.mgz mri/transforms/talairach.m3z > > > /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > > mri/aseg.auto_noCCseg.label_intensities.txt \n > > > > > > relabeling unlikely voxels interior to white matter surface: > > > > > > norm: mri/norm.mgz > > > > > > XFORM: mri/transforms/talairach.m3z > > > > > > GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > > > > > label intensities: mri/aseg.auto_noCCseg.label_intensities.txt > > > > > > SUBJECTS_DIR /Users/nandita/FreeSurfer_recon > > > > > > subject newtemplate > > > > > > outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz > > > > > > useribbon 0 > > > > > > baseoffset 0 > > > > > > RipUnknown 0 > > > > > > Reading lh white surface > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white > > > > > > Reading lh pial surface > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial > > > > > > Loading lh annotations from > > > /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot > > > > > > reading colortable from annotation file... > > > > > > colortable with 36 entries read (originally > > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab) > > > > > > Reading rh white surface > > > > > > /Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white > > > > > > Reading rh pial surface > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial > > > > > > Loading rh annotations from > > > /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot > > > > > > reading colortable from annotation file... > > > > > > colortable with 36 entries read (originally > > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab) > > > > > > Have color table for lh white annotation > > > > > > Have color table for rh white annotation > > > > > > Loading ribbon segmentation from > > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz > > > > > > Building hash of lh white > > > > > > Building hash of lh pial > > > > > > Building hash of rh white > > > > > > Building hash of rh pial > > > > > > Loading aseg from > > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz > > > > > > ASeg Vox2RAS: ----------- > > > > > > -1.00000 0.00000 0.00000 128.00000; > > > > > > 0.00000 0.00000 1.00000-128.00000; > > > > > > 0.00000-1.00000 0.00000 128.00000; > > > > > > 0.00000 0.00000 0.00000 1.00000; > > > > > > mghRead(mri/norm.mgz, -1): could not open file > > > > > > ------------------------- > > > > > > Labeling Slice > > > > > > relabeling unlikely voxels in interior of white matter > > > > > > Segmentation fault > > > > > > Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: > > > Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64 > > > > > > recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019 > > > > > > On inspecting the mri folder, it seems the norm.mgz file has not been > > > created at all. Looking at the flowchart of steps for the recon-all > > > command, norm.mgz is supposed to be created in the CA Normalize step, > > > which requires the talairach.lta in the newtemplate/mri/transforms > > > folder, which seems to be missing as well. The only transform created > > > in the transform folder is talairach.xfm. The talairach.lta is created > > > in the EM GCA registration step. Do I re-run the EM GCA registration, > > > CA normalize, and then contonue from aparc to aseg? I am not sure if > > > since I am creating my own template, the EM GCA step requires any > > > modifications? Any help will be appreciated. > > > > > > Thanks, > > > > > > Nandita > > > > > > > > > _______________________________________________ > > > Freesurfer mailing list > > > Freesurfer@nmr.mgh.harvard.edu > > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > > > > _______________________________________________ > > Freesurfer mailing list > > Freesurfer@nmr.mgh.harvard.edu > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > > > > > > _______________________________________________ > > Freesurfer mailing list > > Freesurfer@nmr.mgh.harvard.edu > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed...
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OK. Does that mean there is not a problem? I'm just not sure if you need anything from us.
On 12/12/2019 7:37 PM, Joshi, Nandita wrote:
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Hi Dr. Greve,
I apologize, maybe I should have been more specific. What I meant to say was, for example, in the attached area and volume distribution graphs over regions, we have most outliers in the superior frontal, and inferior parietal regions. However, on examining these regions for the subjects that are outliers, in comparison to the other subjects that are not, there doesn't seem to be an increased inclusion of voxels in these regions which could possibly explain why these might be outliers compared to the rest of the group.
- Nandita
On 12/12/19, 7:02 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D."mailto:freesurfer-bounces@nmr.mgh.harvard.eduonbehalfofGreve,DouglasN.,Ph.D. <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu>mailto:freesurfer-bounces@nmr.mgh.harvard.eduonbehalfofDGREVE@mgh.harvard.edu wrote:
When you say that you looked at the labels/segmentations and that nothing looked horribly wrong, would that mean that the labels are accurate and that they are not really outliers?
On 12/5/19 7:26 PM, Joshi, Nandita wrote: > External Email - Use Caution > > Hi Dr. Greve, > > Thanks so much for your response. The primary reason for my wanting to do this is due to a large number of outliers in terms of area and volume. Our subject population is cognitively impaired older adults > 60 yrs. The custom surface based template might help in investigating the area outliers, but it wouldn't help in investigating the volume outliers. Since the DK atlas is constructed from a population 18-86 years including 10 people with Alzheimer's Disease, I am not even sure if making a new volume atlas would help. > > Attached are graphs showing the distribution of the region wise area, volume, and thickness of all subjects in lh (filled circles) and rh (open circles). I looked into the aparc and aseg labels in specific regions for the subjects that are outliers, but nothing seems to have gone horribly wrong in terms of parcellation. Any pointers on how I can investigate and if a custom volume template might help? > > Thanks, > Nandita > > > > On 12/5/19, 1:36 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D."mailto:freesurfer-bounces@nmr.mgh.harvard.eduonbehalfofGreve,DouglasN.,Ph.D. <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu>mailto:freesurfer-bounces@nmr.mgh.harvard.eduonbehalfofDGREVE@mgh.harvard.edu wrote: > > We currently do not have a mac build of mri_aparc2aseg, and we recommend > downloading the freesurfer dev release to resolve this issue. > > > > On 12/5/19 1:00 PM, Joshi, Nandita wrote: > > External Email - Use Caution > > > > Thank you so much for that! The README file instructs to download make_average_subject and platform specific mri_aparc2aseg. However, the mac version of mri_aparc2aseg is missing in the patch. Could it be possible to download it from elsewhere? > > > > - Nandita > > > > > > > > On 12/5/19, 12:49 PM, "freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Greve, Douglas N.,Ph.D."mailto:freesurfer-bounces@nmr.mgh.harvard.eduonbehalfofGreve,DouglasN.,Ph.D. <freesurfer-bounces@nmr.mgh.harvard.edu on behalf of DGREVE@mgh.harvard.edu>mailto:freesurfer-bounces@nmr.mgh.harvard.eduonbehalfofDGREVE@mgh.harvard.edu wrote: > > > > USE CAUTION: External Message. > > > > See the REAME file here > > https://urldefense.proofpoint.com/v2/url?u=ftp-3A__surfer.nmr.mgh.harvard.ed... > > > > > > On 12/5/19 11:47 AM, Joshi, Nandita wrote: > > > > > > External Email - Use Caution > > > > > > Hello, > > > > > > I am trying to make a study specific template based on the > > > instructions at: > > > https://urldefense.proofpoint.com/v2/url?u=https-3A__surfer.nmr.mgh.harvard.... > > > > > > However, on running the first step: > > > > > > make_average_subject --out newtemplate --subjects subj1 subj2 subj3 ... > > > > > > I get an error while running the recon-all is trying to run the aparc > > > to aseg step: > > > > > > #@# AParc-to-ASeg aparc Thu Dec5 11:29:33 EST 2019 > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate > > > > > > \n mri_aparc2aseg --s newtemplate --volmask --aseg aseg.presurf.hypos > > > --relabel mri/norm.mgz mri/transforms/talairach.m3z > > > /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > > mri/aseg.auto_noCCseg.label_intensities.txt \n > > > > > > relabeling unlikely voxels interior to white matter surface: > > > > > > norm: mri/norm.mgz > > > > > > XFORM: mri/transforms/talairach.m3z > > > > > > GCA: /Applications/freesurfer/average/RB_all_2016-05-10.vc700.gca > > > > > > label intensities: mri/aseg.auto_noCCseg.label_intensities.txt > > > > > > SUBJECTS_DIR /Users/nandita/FreeSurfer_recon > > > > > > subject newtemplate > > > > > > outvol /Users/nandita/FreeSurfer_recon/newtemplate/mri/aparc+aseg.mgz > > > > > > useribbon 0 > > > > > > baseoffset 0 > > > > > > RipUnknown 0 > > > > > > Reading lh white surface > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.white > > > > > > Reading lh pial surface > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/lh.pial > > > > > > Loading lh annotations from > > > /Users/nandita/FreeSurfer_recon/newtemplate/label/lh.aparc.annot > > > > > > reading colortable from annotation file... > > > > > > colortable with 36 entries read (originally > > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.41916/seg.1.100110189.ctab) > > > > > > Reading rh white surface > > > > > > /Users/nandita/reeSurfer_recon/newtemplate/surf/rh.white > > > > > > Reading rh pial surface > > > > > > /Users/nandita/FreeSurfer_recon/newtemplate/surf/rh.pial > > > > > > Loading rh annotations from > > > /Users/nandita/ADRC/FreeSurfer_recon/newtemplate/label/rh.aparc.annot > > > > > > reading colortable from annotation file... > > > > > > colortable with 36 entries read (originally > > > /Users/nandita/FreeSurfer_recon/newtemplate/label/tmpdir.annot2std.47059/seg.1.100110189.ctab) > > > > > > Have color table for lh white annotation > > > > > > Have color table for rh white annotation > > > > > > Loading ribbon segmentation from > > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/ribbon.mgz > > > > > > Building hash of lh white > > > > > > Building hash of lh pial > > > > > > Building hash of rh white > > > > > > Building hash of rh pial > > > > > > Loading aseg from > > > /Users/nandita/FreeSurfer_recon/newtemplate/mri/aseg.presurf.hypos.mgz > > > > > > ASeg Vox2RAS: ----------- > > > > > > -1.00000 0.00000 0.00000 128.00000; > > > > > > 0.00000 0.00000 1.00000-128.00000; > > > > > > 0.00000-1.00000 0.00000 128.00000; > > > > > > 0.00000 0.00000 0.00000 1.00000; > > > > > > mghRead(mri/norm.mgz, -1): could not open file > > > > > > ------------------------- > > > > > > Labeling Slice > > > > > > relabeling unlikely voxels in interior of white matter > > > > > > Segmentation fault > > > > > > Darwin Nanditas-MacBook-Pro.local 17.7.0 Darwin Kernel Version 17.7.0: > > > Sun Jun2 20:31:42 PDT 2019; root:xnu-4570.71.46~1/RELEASE_X86_64 x86_64 > > > > > > recon-all -s newtemplate exited with ERRORS at Thu Dec5 11:29:52 EST 2019 > > > > > > On inspecting the mri folder, it seems the norm.mgz file has not been > > > created at all. Looking at the flowchart of steps for the recon-all > > > command, norm.mgz is supposed to be created in the CA Normalize step, > > > which requires the talairach.lta in the newtemplate/mri/transforms > > > folder, which seems to be missing as well. The only transform created > > > in the transform folder is talairach.xfm. The talairach.lta is created > > > in the EM GCA registration step. Do I re-run the EM GCA registration, > > > CA normalize, and then contonue from aparc to aseg? I am not sure if > > > since I am creating my own template, the EM GCA step requires any > > > modifications? Any help will be appreciated. > > > > > > Thanks, > > > > > > Nandita > > > > > > > > > _______________________________________________ > > > Freesurfer mailing list > > > Freesurfer@nmr.mgh.harvard.edumailto:Freesurfer@nmr.mgh.harvard.edu > > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > > > > _______________________________________________ > > Freesurfer mailing list > > Freesurfer@nmr.mgh.harvard.edumailto:Freesurfer@nmr.mgh.harvard.edu > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > > > > > > _______________________________________________ > > Freesurfer mailing list > > Freesurfer@nmr.mgh.harvard.edumailto:Freesurfer@nmr.mgh.harvard.edu > > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edumailto:Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed... > > > _______________________________________________ > Freesurfer mailing list > Freesurfer@nmr.mgh.harvard.edumailto:Freesurfer@nmr.mgh.harvard.edu > https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.nmr.mgh.harvard.ed...
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