Hi Jim and Luis - The only change between versions of recon-all that would mess things up for TRACULA would be if the IDs of the structures in the aparc+aseg changed (e.g., the left thalamus changed from 10 to 100) or a new structure got added to the aparc+aseg that wasn't there in the previous version or a structure got removed. As far as I know, this hasn't happened (the recon-all crew can correct me if I'm wrong). So using the output from an older version of recon-all when running a newer version of trac-all should be fine.

Since we now also recommend the thalamic nuclei segmentation for the latest version of TRACULA (when tracts that terminate or neighbor the thalamus are of interest), as Jim said you can always just run the thalamic nuclei segmentation on top of your existing recon-all output.

Best, Anastasia. ________________________________ From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Alvarado,Luis R luis.alvarado@louisville.edu Sent: Sunday, October 31, 2021 6:22 AM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu; dimitriosalexopoulos@wustl.edu dimitriosalexopoulos@wustl.edu Subject: Re: [Freesurfer] Tracula 7.2 using older structural outputs

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Hi all,

I am also curious about this question.

Can you run TRACULA 7.2 using the outputs from FS 6.0 and 7.1.1?

If so, is it ok to run the new segmentThalamicNuclei.sh and segmentBS.sh 7.2 scripts on FS 6.0 recon data and feed that to TRACULA 7.2?

Best,

Luis R. Alvarado Graduate Student Fellow Department of Anatomical Sciences and Neurobiology University of Louisville Kentucky Spinal Cord Injury Research Center 220 Abraham Flexner Way, Suite 1500 Louisville, KY 40202

________________________________ From: Alexopoulos, Dimitrios dimitriosalexopoulos@wustl.edu Sent: Tuesday, August 24, 2021 2:00 PM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: [Freesurfer] Tracula 7.2 using older structural outputs

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HI,

Can you run Tracula 7.2 using older structural outputs generated with FS 6.0 and 7.1.1?

Jim

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