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Hi FreeSurfer community,

I'm running into an issue with the longitudinal FreeSurfer pipeline.

Background:

I'm working with T1-weighted MRI scans from an aerobic exercise intervention study in older adults (age 60+). I have two timepoints per subject: pre- and post-intervention. Cross-sectional `recon-all` has successfully completed for both timepoints. I'm now trying to run the longitudinal pipeline using `recon-all -base`, but I'm getting a glibc/license-related error.

Command:

```bash recon-all -base sub002base \ -tp /rds/projects/s/segaertk-chbh-00077/Structural/FreeSurfer/sub002_tp1 \ -tp /rds/projects/s/segaertk-chbh-00077/Structural/FreeSurfer/sub002_tp2 \ -all ```

Error: ``` GNU libc version: 2.28 ERROR: Systems running GNU glibc version greater than 2.15 require a newly formatted license file (it's free). Please download a new one from the following page: http://secure-web.cisco.com/1S-XRYZwdfIh6LBaYDmryClXzVhMGn3s7IZR6N9Pa3Ony7_a... ```

Notes:

I have a valid, updated license file (downloaded from the official site). I set the license path before running the command:

```bash export FS_LICENSE=${HOME}/license.txt ``` Weirdly, the cross-sectional `recon-all` works fine using the same environment and license file.

I'm using FreeSurfer version: FreeSurfer/6.0.1-centos6_x86_64 My system is running glibc 2.28.

What I've Tried:

* Verified that `$FS_LICENSE` is exported and correct. * Checked for any outdated `license.txt` in `$FREESURFER_HOME` and replaced it. * Confirmed that `recon-all -version` and `which recon-all` return the expected FreeSurfer install. * Deleted partial `sub002base` folder and retried clean.

Has anyone encountered this behaviour - where the cross-sectional `recon-all` works, but `recon-all -base` fails due to the license/glibc issue?

Any help would be greatly appreciated!

Warmest regards,

Foyzul.

Dr Foyzul Rahman Centre for Human Brain Health (CHBH) University of Birmingham United Kingdom