Hi, all After running recon-all with -hippo-subfield several .mgz files are created. I could add each of those files as volumes in freeview , toggle " show as isosurface in 3D view" and select a different color for each kind of subfield and got the attached screenshots. They are very interesting but now I have some doubts. 1- How do I save the combined 3D view I got as a surface? 2-I know the hippo-subifields .mgz files are probability maps and if I guess right kvlQuantifyPosteriorProbabilityImages read these files to count how many voxels you have in each subfield. I also guess it count only the voxels with reasonable high probability to be part of each subfield. If so, what is the minimum value between 1 and 255 the program use to decide if a particular voxel is valid? 3- Is it possible to merge hippo-subfield .mgz files data and to generate a different segmentation like anterior (anterior quarter), body (the2 intermediate quarters) and posterior (posterior quarter).
TY in advance
Dear Marcos,
1- How do I save the combined 3D view I got as a surface?
Do you want a surface of the whole hippocampus? In that case I would suggest adding up the probability volumes from all the subfields and thresholding the resulting volume.
2-I know the hippo-subifields .mgz files are probability maps and if I guess right kvlQuantifyPosteriorProbabilityImages read these files to count how many voxels you have in each subfield. I also guess it count only the voxels with reasonable high probability to be part of each subfield. If so, what is the minimum value between 1 and 255 the program use to decide if a particular voxel is valid?
The estimates of the volumes are based on soft probabilities, so all voxels with non-zero probability contribute towards the estimate. For instance, a voxel with value 255 contributes to the volume 255 times as much as a voxel with a value of 1.
3- Is it possible to merge hippo-subfield .mgz files data and to generate a different segmentation like anterior (anterior quarter), body (the2 intermediate quarters) and posterior (posterior quarter).
There are currently no scripts in FreeSurfer to generate such a partition. But you could always take a mask of the whole hippocampus (either from aseg.mgz of from the combination of all the subfields, like I mentioned in 1) and define some simple geometric rules to create it.
Kind regards,
Eugenio
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