Dear Avi,

thank you for your reply, probably I didn't make my point clear enough. Our high resolution quantitative T1-maps (derived from multispectral FLASH) have excellent GM-WM contrast and once autorecon1 (talairach-avi) gets over this tricky coregistration into standard space all other following steps look very fine (including the subcortical segmentation which I'm interested in). Since registration is often more robust when images are approximately realigned from the beginning, I thought to use SPM (or fsl) affine coregistration parameters (to MNI space) as a guess in talairach_avi would be worth a try (I think the problem lies in a somewhat different contrast of the neck and skull which appear brighter in our qT1 maps compared to MPRAGE). I could use SPM-coregistered T1 maps instead an put them into the freesurfer pipeline which, however, would mean an additional interpolation (which is not what I want). Is there another workaround? ( I read that in tkregister2 there is the option to apply fsl transformation matrices - something like that would be needed - but I'm no too familiar with the way freesurfer handels and stores those transformations). As my doctorate fellow sees himself manually segmenting all the subcortical structurs any help would be greatly appreciated -:)

Thanks,

Simon Baudrexel Brain Imaging Center Goethe University Frankfurt

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Von: Snyder, Avi [avi@npg.wustl.edu] Gesendet: Donnerstag, 12. Juli 2012 01:58 Bis: Baudrexel, Simon; Bruce Fischl Cc: freesurfer@nmr.mgh.harvard.edu Betreff: Re: AW: [Freesurfer] Using SPM transformation matrices as initial guess in talairach_avi

Simon,

The standard Talairach atlas transform module in FreeSurfer was designed for T1W high-resolution structural images (e.g., MP-RAGE or SPGR). This is a design choice partly driven by the fact that the FreeSurfer segmentation algorithm assumes high-resolution T1 weighting. Reliable atlas transformation with any other contrast mechanism would be highly uncertain. Atlas transformation modules theoretically could be produced for other contrast mechanisms, e.g., R1 or FA. However, it is not clear that, even if this were done, that the tissue classification part of FreeSurfer would work. If you have R1 and/or FA images that need to be analyzed using FreeSurfer segmentations, I would think that you should submit a minimally processed high-resolution T1W image to FreeSurfer and then use the generated results as ROIs in subsequent analyses. Of course, this strategy requires that the images containing the contrast to be evaluated are co-registered to the segmented MP-RAGE.

I hope I have correctly understood your scientific objectives.

Avi Snyder ------------ On 7/10/12 4:20 AM, "Baudrexel, Simon" Simon.Baudrexel@kgu.de wrote:

Hi Bruce,

I'm using freesurfer version 5.1.0. I currently try to process quantitative T1 (R1) maps derived from 4 and 18 deg. FLASH images and FA maps. Interestingly, transformation appears to fail less often when using single (16 deg.) FLASH images, only. SPM segmentation routine on the other hand works pretty well on both T1 and FLASH images (probably because of using header origin information - which was set to the anterior commissure- as first guess in the registration).

Thanks Simon ________________________________________ Von: Bruce Fischl [fischl@nmr.mgh.harvard.edu] Gesendet: Montag, 9. Juli 2012 20:03 Bis: Baudrexel, Simon Cc: freesurfer@nmr.mgh.harvard.edu; Avi Snyder Betreff: Re: [Freesurfer] Using SPM transformation matrices as initial guess in talairach_avi

Hi Simon

what version are you using? Avi Snyder (ccd) has been tracking down some interactions between his talairach code and our intensity normalization that seem to have made it more fragilie, particularly in 5.1. He has some suggested fixes that may improve things for you.

cheers Bruce

On Mon, 9 Jul 2012, Baudrexel, Simon wrote:

...

Hello,

since I experienced a high rate of registration errors using talairach_avi in the conventional recon-all processing stream (more than 50% misregistration), I would like to incorporate SPM affine registration parameters (as provided in the seg_inv_sn.mat file obtained from the segmentation routine) as initial guess "to help" talairach-avi. Is there a possibility to do this? How do I have to adjust the transformation matrix that it can be handled by freesurfer?

I also tried to circumvent the registration-problem using skull-stripped images, which unfortunately also did not improve the results (I think a template made from skull-stripped images has to be used in that case)

Any help would be much appreciated

Regards, Simon