Freesurfer

freesurfer@nmr.mgh.harvard.edu

17 participants
30086 discussions

modeling multiple scalars in F-contrasts
by L. Schweren 26 May '14

26 May '14

Dear Freesurfer experts, I am modelling multiple covariates (age, SES, nr of symptoms and nr of symptoms2) in one subject group. For the quadratic term I added a column in the fsgd-file. I want to visualize the effect of nr of symptoms, either their linear or their quadratic effects. I therefore made two contrasts: The effect of symptoms: 0 0 1 0 And the effect of symptoms2: 0 0 0 1 I would like to model these two effects together in one contrast (to get the results of both contrasts overlaid on top of each other, but in one mgh file). I tried the following contrast: 0 0 1 0 0 0 0 1 But the result is very different from the two individual contrasts. I get large (non-overlapping) clusters of significant effect in the two separate contrasts, but with the combined contrasts I get none. In the examples I found online for F-contrasts, they always add up to zero, as they are comparisons between groups. What would be the appropriate contrast if I want to visualize all regions with EITHER a linear OR a quadratic effect of symptoms? Thank you very much for your help! Best wishes, Lizanne

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papers on reliability of volume of indivdual cortical parcellation regions
by Mehul Sampat 26 May '14

26 May '14

Hi Folks, I was able to find a number of papers looking at the reliability of cortical thickness of individual regions (ex: https://surfer.nmr.mgh.harvard.edu/pub/articles/reliability_wonderlick.pdf) I was wondering if there are any papers that have looked at the reliability of the volumes of the individual cortical parcellation regions ? Thanks Mehul

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Re: [Freesurfer] Viewing functional data on the inflated brain
by Sherryse Corrow 23 May '14

23 May '14

Thanks Douglas. We tried that . . . The registration matrix that we need is not created. Here is the output log file (attached). I am a bit new to this so I am hoping it has the information you need. After taking a quick glance at the log file, we noticed there are errors in the log file. Step by step, this is what we did after we ran recon-all: Reg-feat2anat ‹feat OurFeatDirectory.feat ‹subject P209 When we open the folder experiment.feat/reg/freesurfer, there is no anat2exf.register.dat file. However, there is a file called: anat2exf.register.dat.init. Thanks, Sherryse -- Sherryse Corrow, Ph.D. / Postdoctoral Fellow Human Vision and Eye Movement Lab Department of Ophthalmology & Visual Sciences UBC/VGH Eye Care Centre 360A-2550 Willow Street Vancouver, BC, V5Z 3N9 Tel: 604-875-4111 ext 67672 Cell: 604-315-6717 sherryse.corrow(a)eyecarecentre.org On 5/6/14, 9:00 AM, "freesurfer-request(a)nmr.mgh.harvard.edu" <freesurfer-request(a)nmr.mgh.harvard.edu> wrote: >Re: Viewing functional data on the inflated brain

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finding cluster peak vertices
by Milde, Christopher 23 May '14

23 May '14

Dear Freesurfers, Is there a way to smooth already preprocessed functional images* (FEAT, FSL), which I coregistered to the reconstructed surfaces (reg feat2anat), on the cortical surface? Furthermore, I would be interested in finding the cluster peak vertex after smoothing the EPI on the reconstructed surface. I know that Hagler et al 2006 talked about a "vertex search method" to find the maximally activated vertex on the surface. Kindly regards, Christopher *the FEAT preprocessed and 1st level statistics FEATs are not smoothed to avoid blurring across attached gyri

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WG: [Fwd: WG: missing temporal lobes]
by Mayer Kristina 23 May '14

23 May '14

Dear Bruce My dataset is very young (around 2 years of age), I suppose the contrast and size is just different from the default-template of freesurfer. Is there a way to exchange the template (I am thinking of a probabilistic map) without creating an own atlas first? Attached you find screenshots of my work and the recon-log of the same subject. I first run all recon-all steps. Then I added CP where WM was missing. I also added CP in GM region where lost of GM was missing (temporal lobe) to get the pial right. Then I tried to remove the WM which came out too much, for I placed CP in GM regions. Is there another way for it? Best, Kristina log 0001339: Fri May 16 08:50:00 CEST 2014 FREESURFER_HOME /usr/local/freesurfer Actual FREESURFER_HOME /usr/local/freesurfer /media/n-NAS_MRI/freesurfer-subjects-dir/0001336 setenv SUBJECTS_DIR /media/n-NAS_MRI/freesurfer-subjects-dir -autorecon2-cp -autorecon3 -subjid 0001336 Linux cs2.kispi.int 3.13.5-103.fc19.x86_64 #1 SMP Mon Mar 3 18:46:36 UTC 2014 x86_64 x86_64 x86_64 GNU/Linux cputime unlimited filesize unlimited datasize unlimited stacksize 8192 kbytes coredumpsize 0 kbytes memoryuse unlimited vmemoryuse unlimited descriptors 1024 memorylocked 64 kbytes maxproc 1024 maxlocks unlimited maxsignal 514103 maxmessage 819200 maxnice 0 maxrtprio 0 maxrttime unlimited FSL_BIN=/usr/local/fsl/bin SSH_AGENT_PID=4883 XDG_SESSION_ID=35 HOSTNAME=cs2.kispi.int SELINUX_ROLE_REQUESTED= FS_OVERRIDE=0 GPG_AGENT_INFO=/home/maykr/.gnupg/S.gpg-agent:4883:1 GLADE_PIXMAP_PATH=: TERM=xterm-256color XDG_MENU_PREFIX=xfce- SHELL=/bin/bash HISTSIZE=1000 SSH_CLIENT=127.0.0.1 40762 22 FSLMULTIFILEQUIT=TRUE PERL5LIB=/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/usr/local/freesurfer/mni/lib/perl5/5.8.5 SELINUX_USE_CURRENT_RANGE= WINDOWID=46782934 OS=Linux XAPPLRESDIR=/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/X11/app-defaults QT_GRAPHICSSYSTEM_CHECKED=1 http_proxy=http://proxy.uzh.ch:3128/ LOCAL_DIR=/usr/local/freesurfer/local USER=maykr 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LD_LIBRARY_PATH=/opt/MATLAB/MATLAB_Compiler_Runtime/v715/sys/java/jre/glnxa64/jre/lib/amd64/server/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/bin/glnxa64/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/runtime/glnxa64/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/sys/java/jre/glnxa64/jre/lib/amd64/server/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/bin/glnxa64/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/runtime/glnxa64/:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/client:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/server:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/native_threads:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/bin:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/os/glnxa64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/bin/glnxa64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/runtime/glnxa64:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/sys/java/jre/glnxa64/jre/lib/amd64/server/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/bin/glnxa64/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/runtime/glnxa64/:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/client:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/server:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/native_threads:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/bin:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/os/glnxa64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/bin/glnxa64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/runtime/glnxa64:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/sys/java/jre/glnxa64/jre/lib/amd64/server/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/bin/glnxa64/:/opt/MATLAB/MATLAB_Compiler_Runtime/v715/runtime/glnxa64/:/media/n-NAS_MRI/Fontan/programs/ibeat/! MCR/v715 /sys/java/jre/glnxa64/jre/lib/amd64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/client:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/server:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/java/jre/glnxa64/jre/lib/amd64/native_threads:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/bin:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/sys/os/glnxa64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/bin/glnxa64:/media/n-NAS_MRI/Fontan/programs/ibeat/MCR/v715/runtime/glnxa64: FSFAST_HOME=/usr/local/freesurfer/fsfast GLADE_MODULE_PATH=: MNI_PERL5LIB=/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/usr/local/freesurfer/mni/lib/perl5/5.8.5 FMRI_ANALYSIS_DIR=/usr/local/freesurfer/fsfast SSH_AUTH_SOCK=/home/maykr/.gnupg/S.gpg-agent.ssh FTP_PROXY=http://proxy.uzh.ch:3128/ ftp_proxy=http://proxy.uzh.ch:3128/ FSF_OUTPUT_FORMAT=nii SESSION_MANAGER=local/unix:@/tmp/.ICE-unix/4872,unix/unix:/tmp/.ICE-unix/4872 MINC_BIN_DIR=/usr/local/freesurfer/mni/bin XDG_CONFIG_DIRS=/etc/xdg SUBJECTS_DIR=/media/n-NAS_MRI/freesurfer-subjects-dir DESKTOP_SESSION=xfce MAIL=/var/spool/mail/maykr NXSESSIONID=cs2.kispi.int-2000-678D3BAFF4D23A591199EAB35F8614B4 PATH=/usr/local/fsl/bin:/usr/local/freesurfer/bin:/usr/local/freesurfer/fsfast/bin:/usr/local/freesurfer/tktools:/usr/local/fsl/bin:/usr/local/freesurfer/mni/bin:/media/n-NAS_MRI/Fontan/programs/ibeat:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/usr/local/fsl/bin:/usr/local/freesurfer/bin:/usr/local/freesurfer/fsfast/bin:/usr/local/freesurfer/tktools:/usr/local/fsl/bin:/usr/local/freesurfer/mni/bin:/media/n-NAS_MRI/Fontan/programs/ibeat:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/usr/local/fsl/bin:/usr/local/freesurfer/bin:/usr/local/freesurfer/fsfast/bin:/usr/local/freesurfer/tktools:/usr/local/fsl/bin:/usr/local/freesurfer/mni/bin:/usr/local/fsl/bin:/usr/local/freesurfer/bin:/usr/local/freesurfer/fsfast/bin:/usr/local/freesurfer/tktools:/usr/local/fsl/bin:/usr/local/freesurfer/mni/bin:/media/n-NAS_MRI/Fontan/programs/ibeat:/media/n-NAS_MRI/Fontan/programs/ibeat/tools:/usr/local/fsl/bin:/usr/local/freesurfer/bin:/usr/local/freesurfer/fsfast/bin:/usr/local/freesurfer/tktools:/usr/local/fsl/bin:/usr/local/freesurfer/mni/bin:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/home/maykr/.local/bin:/home/maykr/bin SHADOW_XAUTHORITY=/home/maykr/.nx/C-cs2.kispi.int-2000-678D3BAFF4D23A591199EAB35F8614B4/authority FSLMACHTYPE=linux_64-gcc4.8 PWD=/media/n-NAS_MRI/freesurfer-subjects-dir/0001336 FUNCTIONALS_DIR=/usr/local/freesurfer/sessions LANG=C KDE_IS_PRELINKED=1 FSLTCLSH=/usr/local/fsl/bin/fsltclsh KDEDIRS=/usr FSLMACHINELIST= MINC_LIB_DIR=/usr/local/freesurfer/mni/lib SELINUX_LEVEL_REQUESTED= FSLREMOTECALL= https_proxy=http://proxy.uzh.ch:3128/ FSLCONFDIR=/usr/local/fsl/config FSLWISH=/usr/local/fsl/bin/fslwish SSH_ASKPASS=/usr/libexec/openssh/gnome-ssh-askpass SLAVE_PID=4068 HISTCONTROL=ignoredups SHLVL=7 HOME=/home/maykr IBEAT_HOME=/media/n-NAS_MRI/Fontan/programs/ibeat MNI_DIR=/usr/local/freesurfer/mni FREESURFER_HOME=/usr/local/freesurfer FSLDISPLAY=/usr/bin/display FSLCONVERT=/usr/bin/convert no_proxy= HTTP_PROXY=http://proxy.uzh.ch:3128/ LOGNAME=maykr FSLDIR=/usr/local/fsl DBUS_SESSION_BUS_ADDRESS=unix:abstract=/tmp/dbus-g9RDmq1Pw0,guid=f19916051f53a528d10b18f853638272 XDG_DATA_DIRS=/usr/local/share:/usr/share SSH_CONNECTION=127.0.0.1 40762 127.0.0.1 22 FIX_VERTEX_AREA= LESSOPEN=||/usr/bin/lesspipe.sh %s XDG_RUNTIME_DIR=/run/user/1019 DISPLAY=:2000.0 QT_PLUGIN_PATH=/usr/lib64/kde4/plugins:/usr/lib/kde4/plugins GLADE_CATALOG_PATH=: FSLLOCKDIR= MNI_DATAPATH=/usr/local/freesurfer/mni/data FSLOUTPUTTYPE=NIFTI_GZ FSL_DIR=/usr/local/fsl COLORTERM=xfce4-terminal _=/usr/bin/tcsh HOSTTYPE=x86_64-linux VENDOR=unknown OSTYPE=linux MACHTYPE=x86_64 GROUP=ZfMRF HOST=cs2.kispi.int OMP_NUM_THREADS=1 REQUIRE_FS_MATCH=0 ________________________________________ Von: freesurfer-bounces(a)nmr.mgh.harvard.edu [freesurfer-bounces(a)nmr.mgh.harvard.edu]" im Auftrag von "Bruce Fischl [fischl(a)nmr.mgh.harvard.edu] Gesendet: Donnerstag, 22. Mai 2014 14:10 An: Freesurfer support list Betreff: Re: [Freesurfer] missing temporal lobes Hi Kristina that shouldn't be needed. If you upload a dataset we will take a look cheers Bruce On Thu, 22 May 2014, Mayer Kristina wrote: > Hi there, > I tried to put more CP, so all the temporal lobe tissue is in the mask. This will end up in overestimation of white matter. So in a second step you will have to manually adjust WM mask. > In my dataset, after adjusting WM mask, WM ist still little bit oversetimated, but at least i am not missing tissue. > When you find a better option, I am glad to learn about. > Good luck! > > Best, Kristina > ________________________________________ > Von: freesurfer-bounces(a)nmr.mgh.harvard.edu [freesurfer-bounces(a)nmr.mgh.harvard.edu]" im Auftrag von "Bruce Fischl [fischl(a)nmr.mgh.harvard.edu] > Gesendet: Donnerstag, 22. Mai 2014 04:35 > An: Freesurfer support list > Betreff: Re: [Freesurfer] missing temporal lobes > > Hi Eileen > Does adding control points to both temporal lobes fix it? > Cheers > Bruce > >> On May 20, 2014, at 9:34 AM, "Daly, Eileen" <eileen.daly(a)kcl.ac.uk> wrote: >> >> Hello all, >> >> We are having trouble with the output after running recon-all -all -subjid subjectx on our scans. >> Registrations and skull striping look okay, but the white matter and pial surface segmentations leave outportions of the anterior temporal lobe. >> >> After adding control points to the left temporal lobe, we then ran recon-all -autorecon2 –cp -autorecon3 -subjid subjects. >> >> This made the the left lobe look better, but then we spotted new missing bits on the right side >> anterior temporal lobe. >> >> Any ideas on dealing with these missing temporal lobe issues? >> >> Thanks, >> >> Eileen Daly >> _______________________________________________ >> Freesurfer mailing list >> Freesurfer(a)nmr.mgh.harvard.edu >> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer > > _______________________________________________ > Freesurfer mailing list > Freesurfer(a)nmr.mgh.harvard.edu > https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Freesurfer mailing list > Freesurfer(a)nmr.mgh.harvard.edu > https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer > > >

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normalization problem (versions 5.1 and 5.3)
by Maia Pujara 23 May '14

23 May '14

Hi all, After running 200+ subjects through recon-all using version 5.1, all but 30 came back with complete surfaces. The 30 that failed seem to have the same problem with normalization. After re-running these 30 subjects with v5.3, we were able to get a good quality brainmask.mgz output, which was not the case when we ran these subjects through v5.1. See attached for comparisons of v5.1 and v5.3 outputs. Would we need to be adding additional flags to get 5.1 to produce usable results for these subjects? Or might there be some other workaround for this? Thanks in advance! Maia

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Create gifti surfaces from lh.white for MATLAB
by Bastian Cheng 23 May '14

23 May '14

Dear Freesurfer-Experts, I would like to create gifti-surfaces to use in matlab. That's what I am doing so far: mris_convert lh.white lh.white.gii I then try to open it using the "gifti" command (http://www.artefact.tk/software/matlab/gifti/) however, I get an error message from matlab: >>>>>>>>>>> ??? Attempt to reference field of non-structure array. Error in ==> gifti.subsref at 28 varargout{1} = this.data{j}.space.MatrixData; Error in ==> gifti.struct at 17 [values{i,:}] = subsref(this(:), substruct('.',names{i})); Error in ==> gifti.display at 17 eval([display_name ' = struct(this);']); >>>>>>>>>>>>>>> Am I missing something? Is there anything special with the .gii file form FS? Any help is appreciated! Bastian

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Differing OS X version and recon-all
by Chiu, Bryan (PHTH) 22 May '14

22 May '14

Hi, Wanted to check in about Mac OS X and any effects on recon-all that I may not be aware of. A while back the Mac was updated to 10.9.2 from some other version of 10.x.x. I ran half of my recon-all on 10.x.x, and am running my 2nd half on 10.9.2. The update was due to some IT person thinking the older version had security flaws. Will I need to rerun all of my subjects on 10.9.2, or has there been no reported differences between these two versions? Thanks, - Bryan

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mri_vol2vol memory error
by Ben Mooneyham 22 May '14

22 May '14

Hello all, I am doing pre-processing for resting-state fMRI volumes, but I have encountered an error and am unsure of how to correct it: I have previously determined the nonlinear warping transformations from my subjects' anatomical (T1) space to the 2mm MNI template using ANTs. At the end of my pre-processing pipeline, I am trying to transform my subjects' functional volumes (206 TRs of 2sec) into this MNI space by 1) transforming the functional volumes into subject T1 space using mri_vol2vol, and 2) applying the nonlinear warp (from ANTs) to the volumes in subject-T1 space to ultimately have my subjects' functional data in MNI space. However, I am getting an error while running mri_vol2vol, where it "cannot allocate region." I gather from looking around online that this is a memory issue, but I am not sure how to fix it. Thoughts? It is crucial that I be able to translate my functional data into common MNI space before performing second-level analyses... I do not encounter this issue when performing this transformation on functional connectivity maps (where I take an ROI defined in MNI space, transform it into T1, and then subject-EPI space, calculate the FC with the rest of the brain, and then invert the process by transforming back into T1, and finally MNI space). How can I get this to work with a larger file? Thank you in advance for your help, Ben Mooneyham -- Graduate Student UCSB - Department of Psychological & Brain Sciences Building 429 Room 102 (210) 912-6076 bwmooneyham(a)gmail.com

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installation recon-all
by Ashley Rose 22 May '14

22 May '14

Hi Freesurfers, I've downloaded and installed Freesurfer on in VirtualBox running Ubuntu 12.04 (on a Windows 7 host machine). In my shell, though, it's installed with CentOS4 (see below) I've been working through the page on testing the installation. Everything works nicely, until I get to the recon-all commands. When I try having it just run the -autorecon1 stage on its own, I get this error: -------- freesurfer-Linux-centos4_x86_64-stable-pub-v5.3.0 -------- Setting up environment for FreeSurfer/FS-FAST (and FSL) FREESURFER_HOME /usr/local/freesurfer FSFAST_HOME /usr/local/freesurfer/fsfast FSF_OUTPUT_FORMAT nii.gz SUBJECTS_DIR /usr/local/freesurfer/subjects MNI_DIR /usr/local/freesurfer/mni arose@HarryCallahan:/usr/local/freesurfer/subjects/buckner_data/tutorial_subjs$ recon-all -s bert -autorecon1 WARNING: tcsh v6.17.06 has an exit code bug! Please update tcsh! INFO: FreeSurfer build stamps do not match Subject Stamp: freesurfer-Linux-centos4-stable-v5.3.0beta-20130503 Current Stamp: freesurfer-Linux-centos4_x86_64-stable-pub-v5.3.0 INFO: SUBJECTS_DIR is /usr/local/freesurfer/subjects Actual FREESURFER_HOME /usr/local/freesurfer rm: cannot remove `/usr/local/freesurfer/subjects/bert/scripts/recon-all.cmd': Permission denied mv: cannot move `/usr/local/freesurfer/subjects/bert/scripts/recon-all.env' to `/usr/local/freesurfer/subjects/bert/scripts/recon-all.env.bak': Permission denied -rw-rw-rw- 1 root root 805935 May 21 16:53 /usr/local/freesurfer/subjects/bert/scripts/recon-all.log Linux HarryCallahan 3.11.0-20-generic #35~precise1-Ubuntu SMP Fri May 2 21:32:55 UTC 2014 x86_64 x86_64 x86_64 GNU/Linux rm: cannot remove `/usr/local/freesurfer/subjects/bert/scripts/recon-all.done': Permission denied /usr/local/freesurfer/subjects/bert/scripts/IsRunning.lh+rh: Permission denied. arose@HarryCallahan:/usr/local/freesurfer/subjects/buckner_data/tutorial_subjs$ Is there something simple that I do so that the files it creates are automatically allowed to be moved/removed etc? The sudo command doesn't help, unfortunately. Thanks in advance, Ashley

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