Dear Anastasia, I am trying to visualize the tract p-values along one pathway as shown in the tutorial.http://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/TraculaStatistics I have implemented the p-values into a text file but I am uncertain of what the settings for the heat scale thresholds ought to be , i.e. I am unsure of what to set the min, mid, max and offset values if I wish to visualize tract segments significant at < 0.05.Thank you very much for your help,cheers,Cat