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Dear FreeSurfer Team,
I am interested in the answer to the question of Subin below. I don't think this question was answered -- at least I cannot find the reply. If the PET images are already smoothed to 8mm (which is the case with the post-processed ADNI images), does it make any sense to apply PVC with --psf 8 at all on them?
The issue is that ADNI data does not provide the PSF of their scanner/recon method. As such, I am unsure what to do in this case.
Thank you for your help! Best regards Julie
Lee Subin Kristine https://secure-web.cisco.com/1mXLTzu2r41QR6HlFRNtEvHNSVSfe8WLOd5uIUAyc7BZMRbnhvYM_8vluOe_YdF4SKuVdeBv4MwoiyTduWg2kCt7B-wVPgCk6zQN3sTtpUFHmW90RsfF4RqUQF0PZCyYXgX-5C7h6qqvuO9dF5rFoNN3p2VPdpaRGu3Bt1_-8mEjDnbCnjL0loK0cbrrYfd6vQBIiSeTfPpMqfOT9NdSNjvZrhr9aXHy33xWEteIklqpAVYpYsTmO37ghEqpai-_3o22SLx-TK3eG9l3uczX37w/https%3A%2F%2Fwww.mail-archive.com%2Fsearch%3Fl%3Dfreesurfer%40nmr.mgh.harvard.edu%26q%3Dfrom%3A%2522Lee%2BSubin%2BKristine%2522 Wed, 20 Jul 2016 02:29:17 -0700 https://secure-web.cisco.com/18P1Tw0COZDNEtYcEuJXMAL4GATt842M24L2wvu-V1JVmncRuYu8Jmcw1aX8JCQqMB3LQv1U5P_lxU3Ye77cnKRyaDHCTS_Dlr83qeDBazewf-9mL07Wcg3pp82u6SKRFlXdi4A8540L1HYNt5-KFd9NZwUQvx7P5rxv9U9otbv3uEvLwOfJ5YRy_BUjmpq4xOACErBllJRHFa83F1QZaCMDxGq0God-4LKY00NqMaM3qiKfXJDtYBGjY1fSB3A3hPaRysoGS3lcD_J19mQoDrA/https%3A%2F%2Fwww.mail-archive.com%2Fsearch%3Fl%3Dfreesurfer%40nmr.mgh.harvard.edu%26q%3Ddate%3A20160720
Hi Doug,
Thanks for your help!
For your response to question #1 from my previous email(copied below), then if all PET images were smoothed so that they all have a uniform resolution of 8mm FWHM, would I put 8 for the psf flag?
1)*mri_gtmpvc --i pet.nii.gz --reg template.reg.lta--psf FWHM--seg gtmseg.mgz **--default-seg-merge --auto-mask PSF .01--mgx .01--o gtmpvc.output *
(a) For the psf flag, do I have to know exactly the FWHM of the point-spread function of the scanner or would a default value of 6 work for all? I am using PET images from multiple centers and thus each image are from different scanners, and I'm not sure how I would find the fwhm for each scanner.
In theory you need the FWHM for each scanner. The FWHM can also change depending upon recon method and parameters and even the ligand used. If you get it about right, probably it will be close enough.
Also, for the second question (copied below), then does setting it to .01 mean maximum sensitivity to gray matter in each PET voxel, and thus this is the recommended threshold for the most accurate resutls?
(b) For the mgx flag, is the GM threshold .01 an optimal threshold referring to the p-value?(am new to PVEcorrection and asking out of curiosity) What is the difference if I enter .01 versus I don't enter it?
You must enter a threshold. When Muller-Gartner is implemented, there are two stages: (1) orthogonalization (subtracting out non-GM) and (2) rescaling. In the rescaling step, each voxel is divided by the fraction of GM in that voxel. If that fraction is 0, then you will get an error. If it is close to 0, you can get extreme noise amplification in that voxel. Setting it to .01 requires that a voxel have at least 1% GM to be rescaled, otherwise the voxel is set to 0.
Best,
Subin
________________________________ 보낸 사람: Douglas Greve gr...@nmr.mgh.harvard.edu 대신 freesurfer-boun...@nmr.mgh.harvard.edu freesurfer-boun...@nmr.mgh.harvard.edu 보낸 날짜: 2016년 7월 19일 화요일 오전 10:28:42 받는 사람: freesurfer@nmr.mgh.harvard.edu 제목: Re: [Freesurfer] PVE correction tool on Freesurfer 6
the response is in the body f the mail
On 7/18/16 9:27 PM, Lee Subin Kristine wrote:
Hi Doug,
It seems that the email reply you just sent to me was empty. Could you please check your email message again?
Thank you as always,
Subin
________________________________ 보낸 사람: Douglas N Greve gr...@nmr.mgh.harvard.edu<mailto:gr...@nmr.mgh.harvard.edu gr...@nmr.mgh.harvard.edu> 대신 freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu freesurfer-boun...@nmr.mgh.harvard.edu>
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu freesurfer-boun...@nmr.mgh.harvard.edu> 보낸 날짜: 2016년 7월 19일 화요일 오전 1:59:46 받는 사람: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu freesurfer@nmr.mgh.harvard.edu> 제목: Re: [Freesurfer] PVE correction tool on Freesurfer 6
On 07/12/2016 11:03 PM, Lee Subin Kristine wrote:
Hi Doug,
Thanks a lot for the page! It was very helpful.
I have a few questions about one of the commands and a question for one of the output files.
1)*mri_gtmpvc --i pet.nii.gz --reg template.reg.lta--psf FWHM--seg gtmseg.mgz **--default-seg-merge --auto-mask PSF .01--mgx .01--o gtmpvc.output *
(a) For the psf flag, do I have to know exactly the FWHM of the point-spread function of the scanner or would a default value of 6 work for all? I am using PET images from multiple centers and thus each image are from different scanners, and I'm not sure how I would find the fwhm for each scanner.
In theory you need the FWHM for each scanner. The FWHM can also change depending upon recon method and parameters and even the ligand used. If you get it about right, probably it will be close enough.
(b) For the mgx flag, is the GM threshold .01 an optimal threshold referring to the p-value?(am new to PVEcorrection and asking out of curiosity) What is the difference if I enter .01 versus I don't enter it?
You must enter a threshold. When Muller-Gartner is implemented, there are two stages: (1) orthogonalization (subtracting out non-GM) and (2) rescaling. In the rescaling step, each voxel is divided by the fraction of GM in that voxel. If that fraction is 0, then you will get an error. If it is close to 0, you can get extreme noise amplification in that voxel. Setting it to .01 requires that a voxel have at least 1% GM to be rescaled, otherwise the voxel is set to 0.
- From the *gtm.stats.dat *output file, the 5th column(number of PET
voxels in the ROI) of, for example, the ctx-lh-precuneus and ctx-rh-precuneus are 1744 and 1805 respectively. However, in the *?h.aparc.stats* files from recon-all, the 4th column(gray matter volume) of lh-precuneus and rh-precuneus are 5928 and 6125 respectively. I am confused because I thought the number of PET voxels should be equal to the number of GM voxels in the ROI?
You need to scale by the size of the PET voxel.
Also, for output files such as gtm.nii.gz or nopvc.nii.gz, what programs can I open them with? It doesn't seem it is openned with mricron or gedit.
They are nifti files, though you probably don't want to use a nifit viewer. You can load them into matlab with MRIread('file.nii.gz')
Thank you again in adavnce for your help.
Best,
Subin
*보낸 사람:* Douglas N Greve gr...@nmr.mgh.harvard.edu<mailto:gr...@nmr.mgh.harvard.edu gr...@nmr.mgh.harvard.edu> 대신 freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu freesurfer-boun...@nmr.mgh.harvard.edu> freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu freesurfer-boun...@nmr.mgh.harvard.edu> *보낸 날짜:* 2016년 7월 8일 금요일 오전 2:53:31 *받는 사람:* freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu freesurfer@nmr.mgh.harvard.edu> *제목:* Re: [Freesurfer] PVE correction tool on Freesurfer 6 Hi Subin, I just created this page this morning http://secure-web.cisco.com/1KvigXsuD3hyHKjk92q3dRvB-q8U59p-kTraKO56xGNX44o7... do you want to take a look and see if it answers your questions. As for #2, yes you still need to run gtmseg.
On 06/26/2016 07:45 AM, Lee Subin Kristine wrote:
Hello,
I am trying to use PVE correction on preprocessed PET data downloaded from the Alzheimer's Disease Neuroimaging Initiative(ADNI) database. I have a couple of questions:
- I am using the co-registered, averaged image of the dynamic images,
and it seems from your answers here
(https://secure-web.cisco.com/1YfE5sw6IqkPaPVgJHa3pvzOwDjKQNrzaGXppR9K3_joheS...)
for static PET images, #3~5 will be different. Could you explain how I would need to enter the command differently?
- I already have segmentations of my MRI images obtained via
FreeSurfer(v.5.2)'s cortical parcellation. Would I still have to do procedure #1 (gtmeseg --s subject) ? I did try using xcerebralseg because it seemed from the help command that in my case where I already have some FS segmentations that I should use this one:
/You can use your own segmentation or a modified FS segmentation. // //It will be easiest if you modify apas+head.mgz to insert your// //segmentations. apas+head.mgz is created by gtmseg but you can// //create it with // / /xcerebralseg --s $subject --o $SUBJECTS_DIR/$subject/mri/apas+head.mgz/
However when I do try I got the following error:
/ERROR: could not open
/usr/local/freesurfer/subjects/002_S_4213/stats/002_S_4213/mri/apas+head.stats
for writing// //Errno: No such file or directory/
Thanks a lot in advance,
Regards, Subin
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-- Douglas N. Greve, Ph.D. MGH-NMR Centergr...@nmr.mgh.harvard.edu<mailto:gr...@nmr.mgh.harvard.edu gr...@nmr.mgh.harvard.edu> Phone Number: 617-724-2358 Fax: 617-726-7422
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- [Freesurfer] PVE correction tool on Freesurfer 6 https://secure-web.cisco.com/1Ud59bF2cwKe79JxI3nEbIOvMcVEHkFV4SxkM6elM-AkU_XFu5TUh0ZLfEUR066iCOyNgXOOiNNDd6jVUX206OND9s73_Hy4qxXutXa73aRICLxXuFXYvPwGOXJTjUhg9dwhQ3mWx7cxpzE6gd9IYXEEDB8chayHy7m1-O40BCfCx0HYP4TSoy7YbwvBrH0ctQEK9fkG4FDbLaalLVejrW5BfGnQhfm-nO2XyyRmgN3KtjeQxG3fA263vYg0ZFO2zzDqj3eBYtmqsgPqgqPn8lA/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg47892.html Lee Subin Kristine - - Re: [Freesurfer] PVE correction tool on Freesurfer... https://secure-web.cisco.com/1Rk_px02yYHOZsAqDAJt9bzKZCNfOYJaEuw4pkgG2gm_AiVLGqropK8Wn6ujDj-1lpmvlGq4We_iomQg6UfCmvfgrrYvD1DU2f5rOVkf--qX4Dyy39EIeG21oiIb00JlIkjKQxs_rSKoekSb4vG5mrq4BwsS5c2qKkRGVwee4KqY3Tu8ZdggTRNyd7oIpAIjvPgje6RqMdil-Tdyw019gF69qbKw6mMwYp-6anrHY_IyXeS_YanBgwx_W0gsjfGuW6ZF2izc55qXI_uyFyfpweQ/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg48163.html Douglas N Greve - - Re: [Freesurfer] PVE correction tool on Freesu... https://secure-web.cisco.com/1WX-AiRv5lDKEWrGsAMHesIx2YcHnwEcTgp96ZJ5uCGOOA2tBQRtyoDLrktyv5PCKy6GLfmDJkSsBzCjvkLrPrE8G986oQQKY5QiLrfSV-ruEu2Hi8TqUTnDfJkN57Z-1l6c4j1pn1mzI9uWQtQ7Ja2ZSV4SqiaNFAYbGEPTLqP_wrsqdi5EPxf_UwmRJdjV-1Dzc6h1OGt30xuthjGeUAe6r8mfiUiBPdzWqbo-DlIBcPCjxDYV7Gprx1qd0q3uzn6ctRK3gPvygjBUpGM1Pmg/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg48257.html Lee Subin Kristine - - Re: [Freesurfer] PVE correction tool on Fr... https://secure-web.cisco.com/1NVAdYfFe5Bj1rrRImGkc5Y_Poyt1tjw1ZFPgvFIXpbWuXTzmqK1cRvUXlgEXV04wa7Jr2KC_3i6Mh9hzOZ2PQx8Yuzjyp-0W0VyWv5iAIUsTQS8Xhb8YoFeY_vBqvHt38g_7wBDRqbbB59-icDXyJSowGLQ5bGG0rLXzxtkd3h6t-LImir1U3ZdInwodK2PaTui2CNPPxJDeCRfVo_waxGgMZTSontQuo6szjURcTIGufurSOuXdt89Myb3I6eunYFaku5joAQ_u23QCdy86Xw/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg48325.html Douglas N Greve - - Re: [Freesurfer] PVE correction tool o... https://secure-web.cisco.com/1Bk991CCjMuRRLPIdjfq7LgVmxITPQZ3_B5EOsSw9FlvSlmPeBQmBJ83pq-gjSIh73NEcXfNd3H8guYvZ8uc9tOQvKxXO2AIcx0AimmRuOGxQDqS-P4S5daO-ZPtEIzekXTdM55-o9fj9J7cnodyOUVAY1vbChFicBdwurZMnElruqChmBvYbNJWwYtz48KfszO2DCKWZD6xP3_XINDylj4BT7FqhA1Kzyi2tIin4L8ajiW89c-vZmeKv0gsWHj-PDegQk4UEasviifizvZssGQ/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg48363.html Lee Subin Kristine - - Re: [Freesurfer] PVE correction t...
https://secure-web.cisco.com/14jyoKPzI0rUdxVrYj7gX3Lu8Pik2ulwoHfFM5Os88hqNDLwFXTfuOKaeE8x6Fe_wXB76JYrRNUTmOsgqO8UFvd1oZ53qDHeAoQJbiNSsngcE_PSqW4Vdqhzyeo9YZU-Fcf54AcEqNS2HyUpNlP4JHTG67IkJkVpfKsbGb89hUNMvi1jpXjililh42Vzqze3-tWqfx4QT39_eTwZfYHXPWPXtTMmWg_w6KDflFXL_cCiOBgyPiTXBwJfakM4OYU-Rxeb_fWuJJu67jHWa1GFqvA/https%3A%2F%2Fwww.mail-archive.com%2Ffreesurfer%40nmr.mgh.harvard.edu%2Fmsg48364.html Douglas Greve - - Re: [Freesurfer] PVE correcti... Lee Subin Kristine
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