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Please, anyone able to answer these questions. Thanks a lot. Sent from my Samsung Galaxy smartphone. -------- Original message --------From: miracle ozzoude miracooloz@gmail.com Date: 2019-10-21 10:24 (GMT-05:00) To: Douglas N Greve freesurfer@nmr.mgh.harvard.edu Subject: subcortical volume-based pet analysis Hello Experts,I do have couple questions about using mri_glmfit and mri_glmfit-sim for this analysis based on the tutorial page. https://surfer.nmr.mgh.harvard.edu/fswiki/PetSurfer%C2%A01) Do i have to add any other flags when using mri_glmfit for this analysis?mri_glmfit --y ${results_dir}/all.mgx.subctxgm.mni305.sm05.nii.gz --fsgd $pet_fsgd --C $matrix1 --mask ${SUBJECTS_DIR}/fsaverage/mri.2mm/subcort.mask.mgz --glmdir ${results_dir}/sub.pet.B6.glmdir2) When correcting for multiple comparisons, should i use --2spaces or --3spaces?3) I ran a paired ttest analysis using age as regressor of no interest for amyloid uptake. My contrast is "1 0" based on the tutorial https://surfer.nmr.mgh.harvard.edu/fswiki/PairedAnalysis.%C2%A0How do i interpret the direction of contrast if I get a result from mri_glmfit-sim (see below)? This is because it doesn't have the Max column for surface based analysis# Cluster Size(n) Size(mm^3) MNIX MNIY MNIZ Max CWP CWPLow CWPHi 1 12709 101672.0 26.00 -69.00 -41.00 5.46844 0.01037 0.00798 0.01316 Right-Cerebellum-White-MatterThank you. best, Paul