Hi Sekar,
Just use one label at a time.
Then you will need to move all of these into diffusion space before using them for tractography.
Lilla ________________________________ From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Sent: Sunday, May 16, 2021 7:05 PM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
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I followed the command and got the following expression:
Akilas-MacBook-Air:B301001 akilasekar$ isolate_labels.csh --vol aparc+aseg.mgz --outprefix aparc+aseg_labels --L $label
Input file: aparc+aseg.mgz
Output prefix: aparc+aseg_labels
mri_convert aparc+aseg.mgz aparc+aseg.nii.gz
reading from aparc+aseg.mgz...
TR=0.00, TE=0.00, TI=0.00, flip angle=0.00
i_ras = (-1, 0, 0)
j_ras = (0, 0, -1)
k_ras = (0, 1, 0)
writing to aparc+aseg.nii.gz...
Label of interest:
@: Expression Syntax.
After I put the label number in, it says command not found.
Thanks for your help!
Regards,
Akila
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Date: Sunday, 16 May 2021 at 22:54 To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
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Hi Lilla,
Thanks a lot for your help!
isolate_labels.csh --vol aseg.mgz --outprefix aseg_labels --L $label
Do I put the labels 7,8,46,47 where it says labels in the code above?
Can I use the ROI files for co-registration into the diffusion space and then tractography?
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Zollei, Lilla,Ph.D. LZOLLEI@MGH.HARVARD.EDU Sent: Sunday, May 16, 2021 10:49:04 PM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
Yes, you can use that one as well.
Lilla
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Sent: Sunday, May 16, 2021 5:41 PM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
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Sorry for another message,
I have the aparc + aseg file, is that the file I should use?
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Sent: Sunday, May 16, 2021 10:37:04 PM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
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Hi Lilla,
Thanks a lot for your help!
isolate_labels.csh --vol aseg.mgz --outprefix aseg_labels --L $label
Do I put the labels 7,8,46,47 where it says labels in the code above?
Can I use the ROI files for co-registration into the diffusion space and then tractography?
Thanks!
Regards,
Akila
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Zollei, Lilla,Ph.D. LZOLLEI@MGH.HARVARD.EDU Date: Sunday, 16 May 2021 at 22:25 To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
The labels that you are looking for are: 7,8, 46, 47
You can use the below command
isolate_labels.csh --vol aseg.mgz --outprefix aseg_labels --L $label
in order to get these from the aseg volume.
That will result in 4 ROI files that you can use in your further analysis.
Lilla
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Sent: Sunday, May 16, 2021 4:34 AM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
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Hi Lilla,
That would be ideal!
Thanks
Akila
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Zollei, Lilla,Ph.D. LZOLLEI@mgh.harvard.edu Sent: Sunday, May 16, 2021 5:22:08 AM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
Is your question then how to isolate the cerebellar WM/GM labels from aseg?
Lila
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Sent: Friday, May 14, 2021 12:13 PM To: Freesurfer support list freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
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Hey,
Thanks for your message. Yes I have!
Akila
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of Zollei, Lilla,Ph.D. LZOLLEI@MGH.HARVARD.EDU Date: Friday, 14 May 2021 at 16:44 To: freesurfer@nmr.mgh.harvard.edu freesurfer@nmr.mgh.harvard.edu Subject: Re: [Freesurfer] Cerebellum Segementation
Hi Akila,
Have you run recon_all on your structural data?
Lilla
________________________________
From: freesurfer-bounces@nmr.mgh.harvard.edu freesurfer-bounces@nmr.mgh.harvard.edu on behalf of A.R. Sekar ars66@cam.ac.uk Sent: Friday, May 14, 2021 10:35 AM To: freesurfer@nmr.mgh.harvard.edu freesurfer@nmr.mgh.harvard.edu Subject: [Freesurfer] Cerebellum Segementation
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Hello Experts!
This is the first time I am doing tractography and my aim is to do it specifically for the cerebellum. I saw online that FreeSurfurer can segment the cerebellum, to create the ROI but I’m not entirely sure how to go about this. So far I have done the pre-processing, co-registration onto diffusion space and created FOD. It would be great if you could give me advice and any feedback about the pipeline.
Thanks for your help!
Regards,
Akila