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Dear Dr.Bruce Fischl Thx for replying. You told me that inequality of the subregions depends on the code I ran. First off I ran the command: recon-all -subject test -i data_00.nii -all "data_00.nii" is a raw MR image. Then, I ran this command: mris_divide_parcellation test lh aparc.annot splitfile.txt lh.aparc.split And splitfile is a 2-column file I myself made based on the FreeSurFerColorLUT.txt's content according to the description in "mris_divide_parcellation --help". And that's it I would be grateful if you could help me. Kind regards;