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To update my progress, I modified my expert.opts file to only contain a single line (*synthstrip --no-csf*) and successfully ran recon-all using the following command:
*recon-all -subjid sub-${sub}_ses-${ses} -i /path/to/anat.nii.gz -synthstrip -synthseg -expert /path/to/expert.opts -3T -qcache -all*
The results are much improved from before (i.e. more accurate pial and white matter boundaries).
My only remaining question is whether or not I need to include the *-synthstrip -synthseg -expert* flags for the BASE and LONG recon-all commands.
Thanks again.
On Fri, Jun 7, 2024 at 9:10 AM Dan Levitas djlevitas208@gmail.com wrote:
Hi Malte,
Thanks for this helpful information. Based on your response, I have a few follow-up questions.
1). Can the expert file (expert.opts) contain a line for the SynthStrip workflow and another line for the SynthSeg workflow, or do I need separate expert files for each process? Assuming the former, is the following an appropriate example example: *synthstrip --no-csf* *synthseg --o $SUBJECTS_DIR/freesurfer/sub-${sub}_ses-${ses}/mri --threads 4*
2). In addition to adding the *-expert* flag into my recon-all command, do I also need to provide *-noskullstrip* at the end since SynthStrip is overriding the watershed skullstripping, or is that implied by the inclusion of the expert.opts file?
3). Although this is for the cross-sectional (CROSS) portion of my longitudinal pipeline, do the BASE and LONG portions also need the -expert flag?
Dan
On Fri, Jun 7, 2024 at 8:43 AM Hoffmann, Malte,PhD < MHOFFMANN@mgh.harvard.edu> wrote:
Hi Dan,
I'm afraid these options aren't currently easy to find as they are being parsed by scripts/rca-config. You can find a set of recon-all options along with a description in distribution/etc/recon-config.yaml [1].
To pass additional options to SynthStrip, you'll need to specify an expert options file by calling recon-all with the `-expert` flag [2]. That file could then include the line `synthstrip --no-csf`, for example.
Malte
[1] https://github.com/freesurfer/freesurfer/blob/dev/distribution/etc/recon-con... [2] https://surfer.nmr.mgh.harvard.edu/fswiki/recon-all#ExpertOptionsFile
From: freesurfer-bounces@nmr.mgh.harvard.edu < freesurfer-bounces@nmr.mgh.harvard.edu> on behalf of Dan Levitas < djlevitas208@gmail.com> Sent: Thursday, June 6, 2024 10:55 To: Freesurfer support list Subject: [Freesurfer] Incoporating synthstrip and synthseg into recon-all
External Email - Use CautionHello,
I am using freesurfer version 7.4.1, and would like to incorporate the recently introduced synthstrip and synthseg functions into my recon-all command, if that's possible. From this archive message ( https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg75148.html) it seems that adding the -synthstrip option enables synthstrip; however, I don't see this as an option in the recon-all documentation ( https://surfer.nmr.mgh.harvard.edu/fswiki/recon-all) nor as a command line option for recon-all.
Assuming that both synthstrip and synthseg can be used in recon-all, would the command need to look something like this: recon-all -subjid sub-${sub}_ses-${ses} -i $anat_file -3T -synthstrip --no-csf -synthseg -qcache -all
I'm adding the --no-csf synthstrip option based on this comment ( https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg75172.html ).
I should also note that I'm performing a longitudinal analysis, so this specific recon-all pertains to the cross-sectional portion of the workflow.
Thanks,
Dan
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