When viewed on the inflated surface in tksurfer, we are seeing “holes” in annot files we created defining our own ROIs. We first created 6 label files on fsaverage then mapped these 6 label files to 5 different subjects using mri_label2label. Once the 6 label files were mapped to each subject, we used mris_label2annot to create an annot from the 6 labels for each subject. This process all seemed to run without errors. We then used tksurfer to view the annot file on the inflated surface for each subject. “holes” appeared in 1-2 of the regions defined by the label files for each subject. We are concerned that these “holes” imply that not all the surface is being included in the region which will in turn result in not all surface being included in the stats files created with the kris_anatomical_stats program.