23 Nov
2015
23 Nov
'15
2:20 p.m.
There were some recent changes in the build that eliminated the necessity for that library. Casper, can you try using this most recent version and let me know if it works?
ftp://surfer.nmr.mgh.harvard.edu//pub/dist/freesurfer/dev_binaries/centos6_x86_64/mri_coreg
Thanks,
-Zeke
Zeke, is this a new library that we need for v6? doug
On 11/20/2015 09:42 AM, Caspar M. Schwiedrzik wrote:
Hi Doug, when I try to execute mri_coreg, I get the following error message:
error while loading shared libraries: libnetcdf.so.6: cannot open shared object file: no such file or directory
Caspar
2015-11-19 18:49 GMT-05:00 Douglas Greve <greve@nmr.mgh.harvard.edu mailto:greve@nmr.mgh.harvard.edu>:
Can you try mri_coreg instead of fsl? ftp://surfer.nmr.mgh.harvard.edu/transfer/outgoing/flat/greve/mri_coreg On 11/19/15 5:25 PM, Caspar M. Schwiedrzik wrote:Hi! I am trying to align a mean EPI volume and a Bruker magnitude map from a fieldmap measurement using tkregister2, fsl_rigid_register and mri_vol2vol. Unfortunately, it seems that somewhere, most likely during fsl_rigid_register, the image orientation get scrambled, and the output has the wrong orientation. Here is what I get from*tkregister2:* target volume epi.nii movable volume fieldmap0001.nii reg file mag_2_mocovol.register.dat LoadVol 0 ZeroCRAS 0 $Id: tkregister2.c,v 1.121.2.1 2011/03/28 20:25:16 greve Exp $ Diagnostic Level -1 INFO: loading target epi.nii Setting targ orientation to RIA INFO: target does not conform to COR format, so I'm going to reslice to COR. This will not affect the final registration. Ttarg: -------------------- -1.000 0.000 0.000 128.000; 0.000 0.000 1.000 -128.000; 0.000 -1.000 0.000 128.000; 0.000 0.000 0.000 1.000; INFO: loading movable fieldmap0001.nii Setting mov orientation to RIA Tmov: -------------------- -0.547 0.000 0.000 17.500; 0.000 0.000 0.547 -17.500; 0.000 -0.547 0.000 17.500; 0.000 0.000 0.000 1.000; mkheaderreg = 1, float2int = 0 Computing reg from header (and possibly input matrix) ---- Input registration matrix (computed) -------- -1.000 0.000 0.000 -0.587; 0.000 1.000 0.000 -2.150; 0.000 0.000 1.000 -2.188; 0.000 0.000 0.000 1.000; --------------------------------------- ---- Input registration matrix -------- -1.000 0.000 0.000 -0.587; 0.000 1.000 0.000 -2.150; 0.000 0.000 1.000 -2.188; 0.000 0.000 0.000 1.000; Determinant -1 subject = rat RegMat --------------------------- 1.000 0.000 0.000 -0.587; 0.000 1.000 0.000 -2.150; 0.000 0.000 1.000 -2.188; 0.000 0.000 0.000 1.000; FSLOUTPUTTYPE NIFTI_GZ tkreg2FSL: mov det = 0.163555, ref det = 0.154688 INFO: FSL2FreeSurfer: Mov volume is NIFTI with positive det, applying LR flip to registration matrix. INFO: FSL2FreeSurfer: Ref volume is NIFTI with positive det, applying LR flip to registration matrix. here is what I get from fsl_rigid_register: $Id: fsl_rigid_register,v 1.31 2011/03/02 20:16:39 nicks Exp $ Thu Nov 19 17:13:48 EST 2015 fieldmap0001_reg.nii Reference is nifti, not converting Input is nifti, not converting Output is nifti, not converting -------------------------------------- /Freiwald/cschwiedrz/rodent/test/Rat_673b_Baseline/Phasemap calling eval flirt.fsl -in ./fieldmap0001.hdr -out ./fieldmap0001_reg.hdr -bins 256 -cost corratio -searchrx -10 10 -searchry -10 10 -searchrz -10 10 -interp trilinear -dof 6 -ref ./epi.hdr -init initxfm.fslmat -omat ./fieldmap0001_reg.fslmat /Freiwald/cschwiedrz/rodent/test/Rat_673b_Baseline/Phasemap mri_copy_params --pulse fieldmap0001_reg.nii fieldmap0001.nii fieldmap0001_reg.nii only copying pulse parameters WARNING: volume sizes are different in_vol : 80, 80, 22 temp_vol : 64, 64, 64 WARNING: voxel sizes are different in_vol : 0.375, 0.375, 1.1 temp_vol : 0.546875, 0.546875, 0.546875 -------------------------------------- /Freiwald/cschwiedrz/rodent/test/Rat_673b_Baseline/Phasemap tkregister2_cmdl --targ epi.nii --mov fieldmap0001.nii --reg ./fsl_rigid_register.28592/reg.dat --fslreg fieldmap0001_reg.nii.fslmat --noedit tkregister_tcl /Freiwald/lab_files/opt/freesurfer-Linux-centos4_x86_64-stable-pub-v5.1.0-full/tktools/tkregister2.tcl ---- FSL registration matrix -------- 0.987 -0.164 0.002 0.247; 0.157 0.943 -0.295 -5.691; 0.046 0.291 0.956 7.475; 0.000 0.000 0.000 1.000; --------------------------------------- target volume epi.nii movable volume fieldmap0001.nii reg file ./fsl_rigid_register.28592/reg.dat LoadVol 0 ZeroCRAS 0 $Id: tkregister2.c,v 1.121.2.1 2011/03/28 20:25:16 greve Exp $ Diagnostic Level -1 INFO: loading target epi.nii INFO: target does not conform to COR format, so I'm going to reslice to COR. This will not affect the final registration. Ttarg: -------------------- -1.000 0.000 0.000 128.000; 0.000 0.000 1.000 -128.000; 0.000 -1.000 0.000 128.000; 0.000 0.000 0.000 1.000; INFO: loading movable fieldmap0001.nii Tmov: -------------------- -0.547 0.000 0.000 17.500; 0.000 0.000 0.547 -17.500; 0.000 -0.547 0.000 17.500; 0.000 0.000 0.000 1.000; mkheaderreg = 0, float2int = 0 FSLOUTPUTTYPE NIFTI fsl2TkReg: mov det = 0.163555, ref det = 0.154688 INFO: FSL2FreeSurfer: Mov volume is NIFTI with positive det, applying LR flip to registration matrix. INFO: FSL2FreeSurfer: Ref volume is NIFTI with positive det, applying LR flip to registration matrix. ---- Input registration matrix (computed) -------- -0.987 0.046 -0.157 0.692; -0.002 0.956 0.295 -19.148; -0.164 -0.291 0.943 -0.998; 0.000 0.000 0.000 1.000; --------------------------------------- ---- Input registration matrix -------- -0.987 0.046 -0.157 0.692; -0.002 0.956 0.295 -19.148; -0.164 -0.291 0.943 -0.998; 0.000 0.000 0.000 1.000; Determinant -1 subject = subject-unknown RegMat --------------------------- 0.987 0.046 -0.157 0.692; 0.002 0.956 0.295 -19.148; 0.164 -0.291 0.943 -0.998; 0.000 0.000 0.000 1.000; Cleaning up Started at Thu Nov 19 17:13:48 EST 2015 Ended at Thu Nov 19 17:13:50 EST 2015 fsl_rigid_register Done if I check the results here using tkmedit -f epi.nii -aux fieldmap0001_reg.nii the orientation of fieldmap0001_reg.nii is already wrong. here is what I get from mri_vol2vol: movvol fieldmap_rads.nii.gz targvol epi.nii outvol fieldmap_rads_reg.nii.gz regfile mag_2_mocovol.register.dat invert 0 tal 0 talres 2 regheader 0 noresample 0 interp trilinear (1) precision float (3) Gdiag_no -1 Synth 0 SynthSeed 1448209916 Final tkRAS-to-tkRAS Matrix is: 0.987 0.046 -0.157 0.692; 0.002 0.956 0.295 -19.148; 0.164 -0.291 0.943 -0.998; 0.000 0.000 0.000 1.000; Vox2Vox Matrix is: 0.676 -0.108 -0.093 8.999; 0.112 0.646 0.586 -2.959; -0.002 -0.202 1.922 -16.003; 0.000 0.000 0.000 1.000; Resampling Output registration matrix is identity INFO: wAriting registration matrix to fieldmap_rads_reg.nii.gz.reg checking the result with tkregister2 --mov fieldmap_rads_reg.nii.gz --targ epi.nii --reg fieldmap_rads_reg.nii.gz.reg also yields the wrong orientation, obviously. In this case, I am changing the fieldmap to RIA orientation, although it is actually IPL; doing the same steps with the original IPL orientation gives the same faulty results. this is the code: tkregister2 --s rat \ --targ $epi \ --mov fieldmap0001.nii \ --reg mag_2_mocovol.register.dat \ --fslregout initxfm.fslmat \ --noedit \ --regheader \ --mov-orientation $fieldmap_orient \ --targ-orientation $epi_orient flirt_opts="-maxangle 10 -dof 6 -interp trilinear -bins 256 -cost corratio" fsl_rigid_register -r $epi \ -i fieldmap0001.nii \ -o fieldmap0001_reg.nii \ -regmat mag_2_mocovol.register.dat \ -initxfm initxfm.fslmat \ ${flirt_opts} # apply registration to phase mri_vol2vol --mov fieldmap_rads.nii.gz --targ $epi --o fieldmap_rads_reg.nii.gz --reg mag_2_mocovol.register.dat --trilin Any advice would be appreciated. Thanks! 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