Hi Michelle, the time series is an average over the ROI right? If so, then it should be independent of any future registration. In any case, aseg2feat will create an ROI in the native functional space which is where FEAT does its analysis, so I think you're fine in any case. doug

Michelle Umali wrote:

Dear Freesurfers, I am using seeds based on Freesurfer masks to run a PPI analysis and am wondering if I am using reg-feat2anat at the right fsl feat registration step.

First, I ran an fsl analysis to generate a feat directory. Then I ran reg-feat2anat using that feat directory. Next I ran aseg2feat and fslmaths to generate binary masks Next I extracted the timeseries by using the filtered func of the feat and the binary masks from aseg2feat.

So now I want to do another feat analysis, but this time using the extracted timeseries But this timeseries is based on the first feat and associated masks from the reg-feat2anat registration.

1. Is it still valid to use this timeseries in this new feat since FSL

will be running another registration? 2) Do I have to run reg-feat2anat again?

Thanks. Michelle

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