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Hi Randolph,

When you visualize the segmentation produced by samseg on top of its input image, does the segmentation look correct? If so, the problem is very likely not the affine registration code. (If the segmentation looks bad, please try samseg in the latest stable version of FreeSurfer, as we've improved the affine registration step quite a lot compared to earlier versions)

Hope this helps,

Koen

On Tue, Oct 5, 2021 at 4:07 PM daedalus39@aol.com wrote:

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Dear Doug,

The "template_coreg.mgz" image is also upside-down and rotated 90 degrees (X). Can you suggest how I might troubleshoot the initial registration?

I can send a logfile if it helps (not sure if I attach the textfile it will pass through your email filter)

I saw a section in "Samseg.py" concerning SAMSEG_LEGACY_REGISTRATION but I'm not sure if this is relevant.

Sincerely, Randolph Andrews

#

    #
    # Preprocessing (reading and masking of data)
    #
    #

=======================================================================================

    # Read the image data from disk and crop

    # Historically, the template was resaved with a transformed header

and the image-to-image transform # was later extracted by comparing the input image and coregistered template transforms, but shear # cannot be saved through an ITK image, so a better method is to pass the image-to-image transform matrix # directly to samseg. If the SAMSEG_LEGACY_REGISTRATION env var is set, this old method is enabled if os.environ.get('SAMSEG_LEGACY_REGISTRATION') is not None: print('INFO: using legacy (broken) registration option') transformedTemplateFileName = os.path.join(self.savePath, 'template_coregistered_legacy.nii') self.imageBuffers, self.transform, self.voxelSpacing, self.cropping = readCroppedImagesLegacy(self.imageFileNames, transformedTemplateFileName) else: self.imageBuffers, self.transform, self.voxelSpacing, self.cropping = readCroppedImages( self.imageFileNames, os.path.join(self.atlasDir, 'template.nii'), self.imageToImageTransformMatrix )

-----Original Message----- From: Douglas N. Greve dgreve@mgh.harvard.edu To: freesurfer@nmr.mgh.harvard.edu Sent: Sun, Oct 3, 2021 8:04 am Subject: Re: [Freesurfer] SAMSEG output orientation not the same as input images

Can you compare the template_coreg.mgz to the input image; they should be mostly aligned. If not, then the initial registration failed.

On 9/28/2021 2:38 PM, daedalus39@aol.com wrote:

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We have recently installed FS-7.1 and have tested a few “ADNI” images with SAMSEG. The process runs to completion, but the output (seg.mgz) appears upside-down and rotated about 90 about the X-axis. We are passing seg.mgz through BrainStorm to create NiFTI images. We do not believe that this is a BrainStorm issue inasmuch as the same images run through recon-all (fully separate proc) yield output images in the same orientation as the original images. I found a thread from 2012 with a similar output orientation issue, but the I did not see a response that resolved this.

Any solution or insight would be greatly appreciated

Sincerely, Randolph Andrews

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