Hi Wonsang - The new tutorial data has subjects named elmo.2005, elmo.2008, and elmo.2012 - not Diff001, which was from the old tutorial data. So if you're using the new tutorial data you need to set your configuration file accordingly with the new subject names, as shown on the tutorial page:

http://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/Tracula

Hope this helps, a.y

On Thu, 23 Jan 2014, Won Sang Jung wrote:

Hi,   I have a problem running TRACULA in the pre-processing stage.    The error message is the following;   Loading streamline start ROI from /usr/local/freesurfer/subjects/tracula/tutorialdata/diffusion_tutorial/Diff001/dlabel/mni/lh.cst_AS_roi1.flt.nii.gz niiRead(): error opening file /usr/local/freesurfer/subjects/tracula/tutorialdata/diffusion_tutorial/Diff001/dlabel/mni/lh.cst_AS_roi1.flt.nii.gz ERROR: Could not read /usr/local/freesurfer/subjects/tracula/tutorialdata/diffusion_tutorial/Diff001/dlabel/mni/lh.cst_AS_roi1.flt.nii.gz Linux localhost.localdomain 2.6.18-371.1.2.el5 #1 SMP Tue Oct 22 12:51:53 EDT 2013 x86_64 x86_64 x86_64 GNU/Linux   trac-preproc exited with ERRORS at 2014. 01. 23. (목) 18:32:17 KST     I guess this error started to happen after I updated Freesurfer version 5.3.0   (I could successfully run TRACULA with same data with the prior version of Freesurfer)     In my setting,   $TUTORIAL_DATA  --> /usr/local/freesurfer/subjects/tracula/tutorialdata     I tried re-download the file, diffusion_tutorial.tar.gz, from freesurfer server and extracted file again, but still I couldn't find the file "lh.cst_AS_roi1.flt.nii.gz" in Diff001/dlabel/mni folder.     Thank you in advance for your advice.     Wonsang Jung