You can use mri_vol2surf to sample it on the surface, then use mris_seg2annot to create an annotation with it. BTW, you can probably just use the output of mri_vol2surf in your matlab program instead of the hassle of working with annotations

On 9/16/2020 3:40 PM, Paul Dhami wrote:

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Dear Freesurfer community,

apologizes in advance for what may be a simple question.

I have a volume region of interest file in .nii format. I would like to do a surface functional connectivity analysis in a MATLAB toolbox, and to do so, I would need to somehow convert this volume ROI to an .annot file.

I was wondering if Freesurfer has any tools to do this?

Could it be accomplished using a combination of mri_vol2label and then mri_label2annot?

Best, Paul

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