Both do both ways. I would recommend not using the --init-rr option for BOLD images
On 9/15/2020 11:42 AM, Wenzhen Zhao wrote:
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Dear Douglas,
Thank you for your quick response. I just have one more question.
Does the “both-way” transformation only done through mri_coreg command that is embedded in preproc-sess command? Or does “bbregister —init-rr “ rigid transformation also does both-way transformation?
Best, Wenzhen
On Sep 15, 2020, at 11:34 AM, Douglas N. Greve <dgreve@mgh.harvard.edu mailto:dgreve@mgh.harvard.edu> wrote:
On 9/15/2020 11:26 AM, Wenzhen Zhao wrote:
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Dear freesurfer developers, I’m attempting to analyze my resting -state fMRI data, and I followed procedures on your functional connectivity page. When I tried to run preprocessing with my data, but it turns out that default mri_coreg did not register my fMRI template volume onto anatomical scan well. So, I went on to register fMRI to anatomical space on my own using bbregister with (init-rr method - rigid transformation). I re-ran preprocessing without registration (-noreg) to correct motion and slice-timing. My commands looked like below: #bbregister —mov fMRI_template_volume —bold —s subject —init-rr —lta functional2anatomical.lta #preproc-sess -s sess01 -fsd rest -stc siemens -noreg -surface fsaverage lhrh -surf-fwhm 5 -mni305-2mm -vol-fwhm 0 -per-run -force Then, I configured seeds (ex. Hippocampus), and ran following commands: #fcseed-config -segid 17 -fcname left_hippocampus.dat -fsd rest -mean -cfg left_hippocampus.config #fcseed-sess -s sess01 -cfg left_hippocampus.config It did run smoothly, but what I noticed from fcseed-sess script was it was transforming anatomical aseg mask into functional space. I am confused on this part, because during processing step, we only make registration file for transformation from functional space to anatomical space, but fcseed-sess command needs registration file that moves anatomical scan to functional scan.Yes, it just inverts the transform to go in the opposite direction
# Convert segmentation to native functional space (##from fcseed-sess script##) set cmd = (mri_label2vol --seg $seganat \ --reg $reg --temp $template --fillthresh $FillThresh \ --o $seg --pvf $pvf); echo $cmd | tee -a $LLF Then, Later, during mkanalysis-sess step, the command seems to use functional 2 anatomical transformation. Could you please clarify which registration file it uses to process each step that I talked about above? Do I also need to make anatomical to functional transformation registration?No, once it has a transformation in one direction, it has it in both directions
I deeply appreciate your help. Sincerely, Wenzhen
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