Hi list, please see the error reported below. It occurs when I invoke tksurfer.Of note, I have completed without problem fs-fast analysis without error.The .license file is well placed and written.Thanks Stefano tksurfer fsaverage rh inflated -aparc -overlay subj/rest/fc.ROI.surf.rh/ROI/sig.nii.gz -fminmax 1.3 3 thresholds min=1.3 max=3 slope=0.588235 mid=2.15 subject is fsaverage hemi is rh surface is inflated surfer: current subjects dir: /Applications/freesurfer/subjects/subject_prova surfer: not in "scripts" dir ==> using cwd for session root surfer: session root data dir ($session) set to: surfer: /Applications/freesurfer/subjects/fMRI/con_run1 checking for nofix files in 'inflated' Reading image info (/Applications/freesurfer/subjects/subject_prova/fsaverage) Reading /Applications/freesurfer/subjects/subject_prova/fsaverage/mri/orig.mgz surfer: Reading header info from /Applications/freesurfer/subjects/subject_prova/fsaverage/mri/orig.mgz -------------------------------------------------------------------------- ERROR: FreeSurfer license file /Applications/freesurfer/.license not found. If you are outside the NMR-Martinos Center, go to http://surfer.nmr.mgh.harvard.edu to get a valid license file (it's free). If you are inside the NMR-Martinos Center, make sure to source the standard environment.